Polysaccharide Hydrogel-Assisted Biosensing Platforms for Point-of-Care Use
Biosensors,
Год журнала:
2025,
Номер
15(1), С. 13 - 13
Опубликована: Янв. 2, 2025
Point-of-care
(POC)
use
is
one
of
the
essential
goals
biosensing
platforms.
Because
increasing
demand
for
testing
cannot
be
met
by
a
centralized
laboratory-based
strategy,
rapid
and
frequent
at
right
time
place
will
key
to
health
safety.
To
date,
however,
there
are
still
difficulties
in
developing
simple
affordable,
as
well
sensitive
effective,
platform
that
enables
POC
use.
In
terms
materials,
hydrogels,
unique
family
water-absorbing
biocompatible
polymers,
have
emerged
promising
components
development
biosensors.
Combinations
hydrogels
various
additional
applications,
such
hydrophilic
coatings,
nanoscale
filtration,
stimuli-responsive
signal
enhancement,
biodegradation.
this
review,
we
highlight
recent
efforts
develop
hydrogel-assisted
platforms
use,
especially
focusing
on
polysaccharide
like
agarose,
alginate,
chitosan,
so
on.
We
first
discuss
pros
cons
practical
applications
then
introduce
case
studies
test
different
formats,
paper-based
analytical
devices
(PADs),
microfluidic
devices,
independent
believe
analysis
present
review
provides
information
resource-limited
settings.
Язык: Английский
Bimetallic MOF doped with reduced graphene oxide enables rapid detection of chloramphenicol
Journal of Physics Conference Series,
Год журнала:
2025,
Номер
2961(1), С. 012020 - 012020
Опубликована: Фев. 1, 2025
Abstract
A
broad-spectrum
antibiotic
called
chloramphenicol
(CAP)
stops
the
creation
of
bacterial
proteins,
which
bacteria
from
growing
and
reproducing.
When
is
misused,
it
can
cause
major
side
effects,
secondary
infections,
resistance,
delays
in
effective
treatment,
higher
treatment
costs.
As
a
result,
creating
an
electrochemical
sensor
that
quick
sensitive
crucial.
The
electrocatalytic
activity
CAP
determination
was
enhanced
this
work
by
producing
reduced
graphene
oxide
doped
bimetallic
organic
framework
(Fe/Al-MOF/rGO)
nanocomposites
single
pot,
had
more
active
sites
better
electrical
conductivity.
constructed
has
linear
range
20
-
1600
μM
detection
line
(LOD)
1.5
nM
(S/N=3).
Furthermore,
sensor’s
recovery
rate
for
sample
good
enough.
Язык: Английский
Ratiometric fluorescence biosensing of granzyme B based on biomimetic microspheres
Sensors and Actuators B Chemical,
Год журнала:
2025,
Номер
unknown, С. 137492 - 137492
Опубликована: Фев. 1, 2025
Язык: Английский
Combining CRISPR-Cas12a with Microsphere Array-Enhanced Fluorescence for Portable Pathogen Nucleic Acid Detection
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 28, 2025
The
detection
of
food
contamination
in
a
swift
and
sensitive
manner
is
essential
for
safeguarding
public
health.
Clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-based
assays
nucleic
acid
are
renowned
their
high
specificity
convenient,
related
studies
have
focused
on
refining
the
Cas
protein
optimizing
CRISPR
(cr)RNAs
design
within
CRISPR-based
enhancing
sensitivity
detection.
Our
research
offers
innovative
insights
into
fluorescence
signal
output
intensity
from
physical
standpoint,
thereby
presenting
practical
cost-effective
strategy
to
lower
thresholds
assays.
By
layer
microsphere
arrays
was
spread
onto
bottom
microfluidic
chip
enhance
sample
via
self-assembly
microspheres.
Recombinase
polymerase
amplification
(RPA)
used
amplify
target
sequences,
followed
by
crRNA
binding
activate
enzyme,
cleaving
fluorescein
amidite
(FAM)-labeled
reporters
emitting
fluorescent
signal.
method
successfully
identified
SARS-CoV-2
positive
samples
(10
clinical
8
environmental
samples)
distinguished
them
negative
samples.
Meanwhile,
it
detected
4
Shigella
5
In
this
study,
developed
exhibited
limit
(LoD)
75
fM
(POCT
with
USB
camera:
50
fM)
100
fM).
It
also
demonstrated
promising
(100%)
small-sample
validation.
Combined
portable
automated
achieved
using
smartphone
receive
process
signals
obtained
platform
study
not
only
applicable
pathogens
cold-chain
products,
but
extends
pathogen
community
hospitals
resource-limited
areas,
providing
an
efficient
solution
rapid
screening
different
settings.
Moreover,
can
be
changing
RPA
primer
crRNA.
This
provides
paradigm
studying
enhanced
signaling
holds
significant
potential
advance
commercialization
use
sensors.
Язык: Английский
Low-Frequency Optical Signal Enhancement Device Combines CRISPR-Based Assay for Portable S. Pneumoniae Detection
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 9, 2025
Early
detection
and
treatment
of
Streptococcus
pneumoniae
(SPN)
is
crucial
for
patients.
However,
since
nucleic
acid
testing
relies
on
large-scale
equipment
specialized
operators,
challenges
remain
accurate,
fast,
low-cost
SPN
detection.
Here,
we
present
a
point-of-care
(POCT)
device
rapid
accurate
based
low-frequency
optical
signal
enhancement
cluster
regularly
interspaced
short
palindromic
repeats
(CRISPR).
The
spotlight
tube
enables
the
fluorescence
signal,
while
combination
an
artificial
intelligence-assisted
autoexposure
algorithm
homomorphic
filtering
image
processing
method
improves
signal-to-noise
ratio
image,
thus
realizing
highly
sensitive
Nucleic
identification
performed
using
CRISPR-based
crRNAs,
fluorescent
probes
were
constructed
against
IytA
gene
SPN.
And
they
showed
high
specificity
sensitivity
gene.
This
demonstrated
excellent
in
detecting
developed
strategy.
threshold
reached
0.1
fM,
single
time
was
only
40
min.
Specificity
validated
clinical
samples,
test
100%
agreement
with
quantitative
polymerase
chain
reaction
results
from
samples.
provides
device,
which,
novel
DNA
probe
SPN,
indicator
option
POCT
Язык: Английский
Highly sensitive and wash-free human serum albumin detection based on filtration-to-enhancement compound microsphere system
Chemical Engineering Journal,
Год журнала:
2025,
Номер
514, С. 163097 - 163097
Опубликована: Май 3, 2025
Язык: Английский
Probe-free and wash-free strategy for on-site testing of chloramphenicol in food based on differential encodable microspheres separation system
Chen Yang,
Guoqing He,
Shukun Zhao
и другие.
Talanta,
Год журнала:
2025,
Номер
295, С. 128250 - 128250
Опубликована: Май 4, 2025
Язык: Английский
Enzyme-Assisted Solid-Phase Microextraction Coupled with a DNA Nanowalker for Dual-Amplified Detection of Chloramphenicol in Animal-Derived Food Products
Yitong Chen,
Zhuoyue Shen,
Chunxu Tao
и другие.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 21, 2024
Chloramphenicol
(CAP),
an
aminoalcohol
antibiotic,
exerts
its
action
on
bacterial
ribosomes,
thereby
obstructing
protein
synthesis.
However,
the
use
of
CAP
in
husbandry
may
lead
to
excessive
accumulation
animal-derived
food
products.
This
presents
potential
risks
consumer
health.
study
developed
a
novel
dual-amplification
fluorescence
detection
method
by
integrating
enzyme-assisted
solid-phase
microextraction
(SPME)
with
Fe3O4@Au
NP-based
DNA
nanowalker
for
food.
The
combination
quartz
rod-based
SPME
biosensor
and
effectively
eliminated
matrix
interference,
enabling
conversion
enhancement
signals
through
two
cyclic
amplification
processes.
strategy
demonstrated
high
sensitivity
limit
28.1
aM
as
well
wide
linear
range
from
0.1
fM
1
nM
(with
R2
>
0.99).
also
demonstrates
robust
stability
accuracy
detecting
trace
amounts
both
authentic
prepared
positive
samples.
Язык: Английский