Biophysical Studies of Amyloid-Binding Fluorophores to Tau AD Core Fibrils Formed without Cofactors DOI Open Access
Daniela Freitas, Joana Saavedra, Isabel Cardoso

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9946 - 9946

Опубликована: Сен. 15, 2024

Tau is an intrinsically disordered protein involved in several neurodegenerative diseases where a common hallmark the appearance of tau aggregates brain. One approach to elucidate mechanisms behind aggregation has been recapitulate vitro self-assembly process fast and reproducible manner. While seeding prompted by negatively charged cofactors, obtained fibrils are morphologically distinct from those found vivo. The AD core fragment (TADC, 306-378) emerged as new model potential solution for cofactor-free tau. Here, we use TADC further study this combining multiple amyloid-detecting fluorophores fibril bioimaging. We confirmed transmission electron microscopy that forms after quiescent incubation at 37 °C. then employed panel eight amyloid-binding query formed species acquiring their emission spectra. results showed nearly all dyes detect self-assembled species. However, successful monitoring kinetics was limited three (X-34, Bis-ANS, pFTAA) which yielded sigmoidal curves but different half-times, hinting being detected. Altogether, highlights using extrinsic fluorescent probes, alone or combination, tools clarify amyloidogenic proteins.

Язык: Английский

Smartphones as a platform for molecular analysis: concepts, methods, devices and future potential DOI Creative Commons
D. G. Baker,

Jasmine Bernal-Escalante,

Christine Traaseth

и другие.

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Smartphones have the potential to transform chemical and biological analysis by functioning as portable, versatile, democratized platforms for molecular diagnostics. Recent advances in optical detection devices are reviewed.

Язык: Английский

Процитировано

0
A solvatochromic carbon dot with turn-on red emissive fluorescence for multi-mode detection of amyloids DOI
Jintao Zhang, J. P. Du,

Minjie Xie

и другие.

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137537 - 137537

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Recent Advances in Nanomaterials for Enhanced Colorimetric Detection of Viruses and Bacteria DOI Creative Commons
Caroline R. Basso, Mário Tanomaru‐Filho,

Victória D’Amario Gavioli

и другие.

Chemosensors, Год журнала: 2025, Номер 13(3), С. 112 - 112

Опубликована: Март 19, 2025

The increasing prevalence of pathogen outbreaks underscores the urgent need for rapid, accurate, and cost-effective diagnostic tools. Colorimetric detection has gained significant attention among available techniques due to its simplicity, portability, potential point-of-care applications. nanomaterial-based colorimetric field continues evolve, with innovations focusing on improving sensitivity, specificity, robustness, cost-effectiveness, friendly analysis. Additionally, efforts address limitations, such as stability environmental impact, pave way more sustainable reliable solutions. This review highlights recent advances in nanomaterials last five years.

Язык: Английский

Процитировано

0
Thioflavin T: induced circular dichroism and density functional theory as promising approach in amyloid fibril assays DOI
Aurica Precupaş, Iulia Matei

Applied Spectroscopy Reviews, Год журнала: 2025, Номер unknown, С. 1 - 39

Опубликована: Май 11, 2025

Язык: Английский

Процитировано

0
Construction of pyrimidine derivatives-copper enzyme mimics as colorimetric sensing elements for efficient detection of phenolic compounds and hydrogen peroxide DOI

Tong-Qing Chai,

Jiaxin Li, Guoying Chen

и другие.

Journal of Hazardous Materials, Год журнала: 2024, Номер 480, С. 136294 - 136294

Опубликована: Окт. 24, 2024

Язык: Английский

Процитировано

2
Biophysical Studies of Amyloid-Binding Fluorophores to Tau AD Core Fibrils Formed without Cofactors DOI Open Access
Daniela Freitas, Joana Saavedra, Isabel Cardoso

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(18), С. 9946 - 9946

Опубликована: Сен. 15, 2024

Tau is an intrinsically disordered protein involved in several neurodegenerative diseases where a common hallmark the appearance of tau aggregates brain. One approach to elucidate mechanisms behind aggregation has been recapitulate vitro self-assembly process fast and reproducible manner. While seeding prompted by negatively charged cofactors, obtained fibrils are morphologically distinct from those found vivo. The AD core fragment (TADC, 306-378) emerged as new model potential solution for cofactor-free tau. Here, we use TADC further study this combining multiple amyloid-detecting fluorophores fibril bioimaging. We confirmed transmission electron microscopy that forms after quiescent incubation at 37 °C. then employed panel eight amyloid-binding query formed species acquiring their emission spectra. results showed nearly all dyes detect self-assembled species. However, successful monitoring kinetics was limited three (X-34, Bis-ANS, pFTAA) which yielded sigmoidal curves but different half-times, hinting being detected. Altogether, highlights using extrinsic fluorescent probes, alone or combination, tools clarify amyloidogenic proteins.

Язык: Английский

Процитировано

0