Synthesis and Characterization of Molecularly Imprinted Polymers for the Selective Extraction of Triterpenoid Saponin From Centella asiatica Crude Extracts DOI

Yongxian Fan,

R. E. Chen,

Lixin Tang

и другие.

Journal of Separation Science, Год журнала: 2025, Номер 48(4)

Опубликована: Апрель 1, 2025

ABSTRACT Asiaticoside (AS) and madecassoside (MS) are two key triterpenoid saponin compounds found in Centella asiatica . Despite their structural similarities, they exhibit distinct biological activities. AS is known for its anti‐inflammatory properties effectiveness treating rheumatoid arthritis, while MS recognized ability to promote wound healing significantly improve burn scars. Additionally, widely utilized the cosmetics industry due antioxidant moisturizing properties. Currently, separating purifying these simultaneously using existing methods challenging. This study focuses on synthesizing molecularly imprinted polymers (MIPs) through precipitation polymerization with as template. By optimizing various preparation parameters, such molar ratios of functional monomers, porogens, cross‐linking agents, high selectivity was achieved. The maximum adsorption capacity reached 19.16 mg/g, an imprinting factor 2.86. MIPs were characterized techniques like scanning electron microscopy (SEM), TA, Brunauer–Emmett–Teller (BET) adsorption, Fourier‐transform infrared (FT‐IR). Their assessed static dynamic desorption, reusability tests. When applied solid‐phase extraction (MISPE) crude extracts from C. , recovery 39.90%, purity increased by 3.01 times, whereas improved 6.51 times. These findings indicate that cost‐effective, highly selective, reproducible, user‐friendly, making it a promising method enriching isolating complex mixtures.

Язык: Английский

Lipopolysaccharide Imprinted Polymers for Specific Recognition of Bacterial Outer Membrane Vesicles DOI
Xiaojun Li,

Xiulei Qi,

Xingguo Liu

и другие.

Analytical Chemistry, Год журнала: 2024, Номер 96(49), С. 19803 - 19811

Опубликована: Ноя. 22, 2024

Outer membrane vesicles (OMVs) secreted by bacteria are emerging diagnostic markers for bacterial infection or disease detection due to their carriage of various signaling molecules. However, actual biological samples patients extremely complex, and applying OMVs clinical diagnosis remains a major challenge. One the challenges is that there still great difficulties in enrichment including tedious steps lower concentration. And some commonly used exosome methods, such as ultracentrifugation, have shortcomings. Herein, we introduce lipopolysaccharide (LPS) molecularly imprinted polymer (MIP) efficient capturing analyzing OMVs, enabling novel approach based on biorecognition materials. LPS, unique structure Gram-negative bacteria, also widely expressed surface which will form cyclic hydrogen bonds with functional monomers MIP affinity interactions. The prepared efficiently can isolate from 100 μL culture broth via specific LPS less than 40 min recovery rate over 95%. Moreover, exhibits good reusability, almost identical performance after 5 repeated cycles, contributing reducing experimental costs both time economy. captured be detected using Western blotting target protein antibodies combination proteomic analysis, providing biomarker platform early diagnosis.

Язык: Английский

Процитировано

4
Evaluation of selective adsorption and permeation behavior for berberine in surface molecular imprinted membrane DOI

Yuan Ma,

Lujie Wang, Qian Zhao

и другие.

Journal of Water Process Engineering, Год журнала: 2025, Номер 71, С. 107265 - 107265

Опубликована: Фев. 24, 2025

Язык: Английский

Процитировано

0
Multi-functional Eu-MOF molecularly imprinted membrane for filtration-enhanced simultaneous adsorption and detection of ribavirin DOI

Junlei Zheng,

Lin Nie,

Yukui Tong

и другие.

Separation and Purification Technology, Год журнала: 2025, Номер unknown, С. 132440 - 132440

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Synthesis and Characterization of Molecularly Imprinted Polymers for the Selective Extraction of Triterpenoid Saponin From Centella asiatica Crude Extracts DOI

Yongxian Fan,

R. E. Chen,

Lixin Tang

и другие.

Journal of Separation Science, Год журнала: 2025, Номер 48(4)

Опубликована: Апрель 1, 2025

ABSTRACT Asiaticoside (AS) and madecassoside (MS) are two key triterpenoid saponin compounds found in Centella asiatica . Despite their structural similarities, they exhibit distinct biological activities. AS is known for its anti‐inflammatory properties effectiveness treating rheumatoid arthritis, while MS recognized ability to promote wound healing significantly improve burn scars. Additionally, widely utilized the cosmetics industry due antioxidant moisturizing properties. Currently, separating purifying these simultaneously using existing methods challenging. This study focuses on synthesizing molecularly imprinted polymers (MIPs) through precipitation polymerization with as template. By optimizing various preparation parameters, such molar ratios of functional monomers, porogens, cross‐linking agents, high selectivity was achieved. The maximum adsorption capacity reached 19.16 mg/g, an imprinting factor 2.86. MIPs were characterized techniques like scanning electron microscopy (SEM), TA, Brunauer–Emmett–Teller (BET) adsorption, Fourier‐transform infrared (FT‐IR). Their assessed static dynamic desorption, reusability tests. When applied solid‐phase extraction (MISPE) crude extracts from C. , recovery 39.90%, purity increased by 3.01 times, whereas improved 6.51 times. These findings indicate that cost‐effective, highly selective, reproducible, user‐friendly, making it a promising method enriching isolating complex mixtures.

Язык: Английский

Процитировано

0