Journal of Food Composition and Analysis, Год журнала: 2025, Номер unknown, С. 107825 - 107825
Опубликована: Май 1, 2025
Язык: Английский
Journal of Food Composition and Analysis, Год журнала: 2025, Номер unknown, С. 107825 - 107825
Опубликована: Май 1, 2025
Язык: Английский
Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 22, 2025
Detecting trace amounts of aflatoxin B1 (AFB1), one the most toxic food contaminants, is crucial for efficiently preventing potential health risks. Circular aptamers are promising candidates bioanalytical applications due to their enhanced biological and structural stability as well compatibility with rolling circle amplification (RCA). Herein, we employed a high-efficiency magnetic chain graphene oxide-based SELEX generate circular that bind AFB1 high affinity selectivity. Notably, selected aptamer, CAFB1-A-2, exhibited significant sequence similarity classical linear aptamer but demonstrated distinct thermodynamic mechanism binding. The binding CAFB1-A-2 was driven by both enthalpy entropy, whereas notable entropy loss required greater compensation. Utilizing this developed sensitive detection system featuring an RCA-assisted allosteric DNAzyme biosensor limit (LOD) 265 pM. Furthermore, constructed aptasensor successfully detected in spiked rice corn, achieving LODs 11 9 μg/kg, respectively, within approximately 4 h. This study provides reliable alternative development AFB1, holding great field safety detection.
Язык: Английский
Процитировано
2Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 161977 - 161977
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
1Microporous and Mesoporous Materials, Год журнала: 2025, Номер unknown, С. 113609 - 113609
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0Journal of Food Composition and Analysis, Год журнала: 2025, Номер unknown, С. 107825 - 107825
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0