Preferential use of organic acids over sugars by soil microbes in simulated root exudation

Soil Biology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109738 - 109738

Опубликована: Фев. 1, 2025

Язык: Английский

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Soil fungi remain active and invest in storage compounds during drought independent of future climate conditions

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Ноя. 29, 2024

Язык: Английский

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Beyond growth: The significance of non-growth anabolism for microbial carbon-use efficiency in the light of soil carbon stabilisation

Soil Biology and Biochemistry, Год журнала: 2024, Номер 193, С. 109400 - 109400

Опубликована: Март 19, 2024

Microbial carbon-use efficiency (CUE) in soils captures carbon (C) partitioning between anabolic biosynthesis of microbial metabolites and catabolic C emissions (i.e. respiratory waste). The use for provides a potential the accumulation metabolic residues soil. Recognised as crucial control cycling, CUE is implemented majority soil models. Due to models' high sensitivity CUE, reliable projections demand accurate quantifications. Current measurements neglect non-growth metabolites, such extracellular polymeric substances (EPS) or exoenzymes, although they remain could be quantitatively important. Here, we highlight that disregarding anabolism can lead severe underestimations CUE. Based on two case studies, demonstrate neglecting exoenzyme EPS production underestimates by more than 100% up 30%, respectively. By incorporating these case-specific values model simulations, observed projects 34% larger SOC stocks over period 64 years when are considered estimating highlighting importance quantification. Our considerations outlined here challenge current ways how measured suggest improvements concerning quantification metabolites. Research efforts should focus (i) advancing estimations capturing multitude uses, (ii) improving techniques quantify products soil, (iii) providing an understanding dynamic uses under different environmental conditions time. In light discussion stabilisation mechanisms, call open 'black box' physiology incorporate all quantitative important measurements.

Язык: Английский

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Development of a Gas Chromatography–Mass Spectrometry Method for the Determination of Ergosterol in Forest Soils

European Journal of Soil Science, Год журнала: 2025, Номер 76(2)

Опубликована: Март 1, 2025

ABSTRACT Ergosterol is a sterol compound present in fungal cell membranes and used as biomarker to measure biomass. Therefore, an accurate analytical method essential quantify the presence of ergosterol any soil type, regardless its physicochemical characteristics or environmental conditions. The main objective our study was optimise validate for assessment total content soil. Different parameters were evaluated during optimisation extract from agricultural forest soils. accuracy by recovery spiked at two levels, resulting good recoveries both types Gas chromatography–mass spectrometry analyse extracts without requiring derivatization step. This simplifies procedure avoids use toxic reagents. applied determine soils taken depths (0–5 cm 5–10 cm). Significant differences concentrations top deeper layers observed. quantity upper layer ranged 3.0 29.9 mg/kg dry weight, whereas soil, it 0.5 4.8 weight. principal component analysis performed between concentration displayed correlation organic matter.

Язык: Английский

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A comparison among EL-FAME, PLFA, and quantitative PCR methods to detect changes in the abundance of soil bacteria and fungi

Soil Biology and Biochemistry, Год журнала: 2024, Номер 198, С. 109557 - 109557

Опубликована: Авг. 19, 2024

Язык: Английский

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Revisiting soil fungal biomarkers and conversion factors: Interspecific variability in phospholipid fatty acids, ergosterol and rDNA copy numbers

Soil Ecology Letters, Год журнала: 2024, Номер 6(4)

Опубликована: Апрель 12, 2024

Abstract Refined conversion factors for soil fungal biomarkers are proposed. High interspecific variability is present in all biomarkers. A modeling approach supports the validity of biomarker estimates diverse soils. ITS1 copies vary strongly, but fungal-specific with least phylogenetic bias. combination will reveal physiology and activity. The abundances fungi bacteria used as simple predictors carbon dynamics, represent widely available microbial traits. Soil serve quantitative these groups, though not quantifying biomass per se. accurate to pools, an understanding its comparability among soils therefore needed. We refined classical biomarkers, evaluated application PCR (qPCR, rDNA copies) a fungi. Based on contents pure cultures 30 isolates tested here, combined comparable published datasets, we propose average 95.3 g C −1 ergosterol, 32.0 mg µmol PLFA 18:2ω6,9 0.264 pg DNA copy . As expected, was most pronounced copies, qPCR results showed based exemplary agricultural further supported hypothesis that high diversity buffers against variability, whereas also biases impact accuracy comparisons estimates. Our analyses suggest cover community best, only partly offset highly ergosterol quantify Ascomycota Basidiomycota To conclude, ecological interpretation coverage data prior their global models important, where different may be insightful.

Язык: Английский

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Xizang meadow degradation alters resource exchange ratio, network complexity, and biomass allocation tradeoff of arbuscular mycorrhizal symbiosis

New Phytologist, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 29, 2024

Summary The response of arbuscular mycorrhizal (AM) symbiosis to environmental fluctuations involves resource exchange between host plants and fungal partners, associations different AM taxa, biomass allocation spore hyphal structures; yet a systematic understanding these responses meadow degradation remains relatively unknown, particularly in Xizang alpine meadow. Here, we approached this knowledge gap by labeling dual isotopes air 13 CO 2 soil 15 NH 4 Cl, computing ecological networks communities, quantifying among spores, intra‐ extraradical hyphae. We found that the ratio photosynthate nitrogen fungi increased with increasing severity degradation, indicating greater dependence on for acquisition. Additionally, using 18S rRNA gene metabarcoding, co‐occurrence were more complex degraded meadows, supporting stress gradient hypothesis. Meadow also toward traits associated hyphae at expense spores. Our findings suggest an integrated consideration exchange, networks, may be important restoration ecosystems.

Язык: Английский

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Systematic review and bibliometric analysis of innovative approaches to soil fertility assessment and mapping: trends and techniques

Applied Geomatics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Язык: Английский

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Manuscript for Soil Biology and Biochemistry Special Issue on “Linking Soil Organic Matter with Soil Health and Decarbonization” Intracellular carbon storage of microorganisms and resulting C sequestration in biosolids-amended agricultural soil

Soil Biology and Biochemistry, Год журнала: 2025, Номер unknown, С. 109836 - 109836

Опубликована: Апрель 1, 2025

Язык: Английский

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Efficiency evaluation of phospholipid fatty acid method based on lipid standards: methanol failed to recover a majority of phospholipids yet eluted unexpected glycolipid

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Май 14, 2025

Phospholipid fatty acid (PLFA) method has been popular and powerful for characterizing soil microbial communities over decades. However, little is known about efficiencies of acidic vs. alkaline extractants catalysts in PLFA extraction methylation. More urgently, it remains unclear whether methanol could efficiently elute phospholipids while avoiding recovering non-target lipids. Here, by adding pure lipid standards representative major neutral, glyco-, into soils collected from subtropical China, we comprehensively evaluated extraction, elution, methylation three steps the method. A good proportion PLFAs be extracted phosphate buffer with both (42-51%) (43-68%) citrate worked better (43-46%) than (36-47%) soils. Phospholipids were expected to mainly eluted methanol, yet found a non-negligible chloroform (36-71%) (9-55%) soils, which much larger previously reported. Only 42-50% (acidic soils) 45-68% (alkaline recovered methanol. Meanwhile, 16% 5% glycolipid DGDG unexpectedly The catalyst (mean 86% across all investigated phospholipids) was more efficient facilitating one 67%). Overall, incomplete separation among types caused loss introduction interferences fraction, leading biased estimation biomass composition. Chloroform elution challenges principle "like dissolves like" solid-phase chromatography, although cannot rule out uncertainty background future experiments are needed provide evidence. To effectively remove phospholipids, possible solutions include replacing hexane, increasing volumes acetone and/or using anion exchange columns.

Язык: Английский

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