Communications Biology,
Год журнала:
2022,
Номер
5(1)
Опубликована: Апрель 29, 2022
Abstract
Heart
organoids
have
the
potential
to
generate
primary
heart-like
anatomical
structures
and
hold
great
promise
as
in
vitro
models
for
cardiac
disease.
However,
their
properties
not
yet
been
fully
studied,
which
hinders
wide
spread
application.
Here
we
report
development
of
differentiation
systems
ventricular
atrial
heart
organoids,
enabling
study
diseases
with
chamber
defects.
We
show
that
our
chamber-specific
comprising
major
cell
types,
use
single
RNA
sequencing
together
sample
multiplexing
characterize
cells
generate.
To
end,
developed
a
machine
learning
label
transfer
approach
leveraging
type,
chamber,
laterality
annotations
available
human
fetal
cells.
then
used
this
model
analyze
organoid
from
an
isogeneic
line
carrying
Ebstein’s
anomaly
associated
genetic
variant
NKX2-5
,
successfully
recapitulated
disease’s
atrialized
In
summary,
established
workflow
integrating
computational
analysis
normal
disease
states.
Life,
Год журнала:
2022,
Номер
12(8), С. 1117 - 1117
Опубликована: Июль 25, 2022
Cardiovascular
diseases
are
a
leading
cause
of
death
worldwide.
Current
treatments
directed
at
heart
repair
have
several
disadvantages,
such
as
lack
donors
for
transplantation
or
non-bioactive
inert
materials
replacing
damaged
tissue.
Because
the
natural
regeneration
cardiomyocytes,
new
treatment
strategies
involve
stimulating
tissue
regeneration.
The
basic
three
elements
cardiac
engineering
(cells,
growth
factors,
and
scaffolds)
described
in
this
review,
with
highlight
on
role
artificial
scaffolds.
Scaffolds
tridimensional
porous
structures
that
imitate
extracellular
matrix,
ability
to
promote
cell
adhesion,
migration,
differentiation,
proliferation.
In
heart,
there
is
an
important
requirement
provide
scaffold
cellular
attachment,
but
scaffolds
also
need
permit
mechanical
contractility
electrical
conductivity.
For
researchers
working
engineering,
choose
adequate
biofabrication
technique,
well
ideal
biocompatible
biodegradable
biomaterial
construction.
Finally,
many
suitable
options
obtain
cell-electrical
interactions
repair,
reaching
goal
engineering.
Circulation Research,
Год журнала:
2022,
Номер
130(12), С. 1780 - 1802
Опубликована: Июнь 9, 2022
An
ensemble
of
in
vitro
cardiac
tissue
models
has
been
developed
over
the
past
several
decades
to
aid
our
understanding
complex
cardiovascular
disorders
using
a
reductionist
approach.
These
approaches
often
rely
on
recapitulating
single
or
multiple
clinically
relevant
end
points
dish
indicative
pathophysiology.
The
possibility
generate
disease-relevant
and
patient-specific
human
induced
pluripotent
stem
cells
further
leveraged
utility
as
screening
tools
at
large
scale.
To
elucidate
biological
mechanisms
models,
it
is
critical
integrate
physiological
cues
form
biochemical,
biophysical,
electromechanical
stimuli
achieve
desired
tissue-like
maturity
for
robust
phenotyping.
Here,
we
review
latest
advances
directed
cell
differentiation
derive
wide
gamut
types,
allow
customization
model
systems,
study
diseased
states
types.
We
also
highlight
recent
progress
development
such
organoids,
microtissues,
engineered
heart
tissues,
microphysiological
systems.
expand
discussion
defining
context
use
selection
currently
available
models.
Last,
discuss
limitations
challenges
with
current
state-of-the-art
future
directions.
Communications Biology,
Год журнала:
2022,
Номер
5(1)
Опубликована: Апрель 29, 2022
Abstract
Heart
organoids
have
the
potential
to
generate
primary
heart-like
anatomical
structures
and
hold
great
promise
as
in
vitro
models
for
cardiac
disease.
However,
their
properties
not
yet
been
fully
studied,
which
hinders
wide
spread
application.
Here
we
report
development
of
differentiation
systems
ventricular
atrial
heart
organoids,
enabling
study
diseases
with
chamber
defects.
We
show
that
our
chamber-specific
comprising
major
cell
types,
use
single
RNA
sequencing
together
sample
multiplexing
characterize
cells
generate.
To
end,
developed
a
machine
learning
label
transfer
approach
leveraging
type,
chamber,
laterality
annotations
available
human
fetal
cells.
then
used
this
model
analyze
organoid
from
an
isogeneic
line
carrying
Ebstein’s
anomaly
associated
genetic
variant
NKX2-5
,
successfully
recapitulated
disease’s
atrialized
In
summary,
established
workflow
integrating
computational
analysis
normal
disease
states.
