Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition DOI Creative Commons
Lei Gu,

Xumiao Li,

Wencheng Zhu

и другие.

Journal of Pharmaceutical Analysis, Год журнала: 2023, Номер 13(8), С. 942 - 954

Опубликована: Май 12, 2023

Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development. However, single-cell proteomic research in this field is relatively underdeveloped because higher threshold starting material for mammalian samples and lack hypersensitive proteome technology. In study, a comprehensive solution ultrasensitive technology (CS-UPT) was developed mouse oocyte/embryo samples. The deep coverage high-throughput routes significantly reduced were selected by investigators based on their demands. Using route, we provided first large-scale snapshot very early stage maternal-to-zygotic transition, including almost 5,500 protein groups from 20 oocytes zygotes each sample. Moreover, significant regulatory networks centered transcription factors kinases between MII oocyte 1-cell rich insights into minor zygotic genome activation.

Язык: Английский

8C-like cells capture the human zygotic genome activation program in vitro DOI Creative Commons
Jasmin Taubenschmid, Maria Rostovskaya, Fátima Santos

и другие.

Cell stem cell, Год журнала: 2022, Номер 29(3), С. 449 - 459.e6

Опубликована: Фев. 24, 2022

The activation of the embryonic genome marks first major wave transcription in developing organism. Zygotic (ZGA) mouse 2-cell embryos and 8-cell humans is crucial for development. Here, we report discovery human 8-cell-like cells (8CLCs) among naive stem cells, which transcriptionally resemble embryo. They express ZGA markers, including ZSCAN4 LEUTX, transposable elements, such as HERVL MLT2A1. 8CLCs show reduced SOX2 levels can be identified using TPRX1 H3.Y marker proteins vitro. Overexpression factor DUX4 spliceosome inhibition increase ZGA-like transcription. Excitingly, 8CLC markers are also expressed ZGA-stage may thus relevant vivo. provide a unique opportunity to characterize might critical insights into early events embryogenesis humans.

Язык: Английский

Процитировано

119
Remodeling of maternal mRNA through poly(A) tail orchestrates human oocyte-to-embryo transition DOI Creative Commons
Yusheng Liu, Han Zhao,

Fanghong Shao

и другие.

Nature Structural & Molecular Biology, Год журнала: 2023, Номер 30(2), С. 200 - 215

Опубликована: Янв. 16, 2023

Abstract Poly(A)-tail-mediated post-transcriptional regulation of maternal mRNAs is vital in the oocyte-to-embryo transition (OET). Nothing known about poly(A) tail dynamics during human OET. Here, we show that length and internal non-A residues are highly dynamic OET, using poly(A)-inclusive RNA isoform sequencing (PAIso-seq). Unexpectedly, undergo global remodeling: after deadenylation or partial degradation into 3ʹ-UTRs, they re-polyadenylated to produce polyadenylated intermediates, coinciding with massive incorporation residues, particularly long consecutive U newly synthesized tails. Moreover, TUT4 TUT7 contribute these BTG4-mediated produces substrates for mRNA re-polyadenylation, TENT4A TENT4B incorporate G residues. The remodeling further confirmed PAIso-seq2. Importantly, essential first cleavage embryos. Together, findings broaden our understanding

Язык: Английский

Процитировано

45
Epigenetic regulation of early human embryo development DOI Creative Commons
Amy L. Wilkinson, Irene Zorzan, Peter J. Rugg‐Gunn

и другие.

Cell stem cell, Год журнала: 2023, Номер 30(12), С. 1569 - 1584

Опубликована: Окт. 18, 2023

Studies of mammalian development have advanced our understanding the genetic, epigenetic, and cellular processes that orchestrate embryogenesis uncovered new insights into unique aspects human embryogenesis. Recent studies now produced first epigenetic maps early embryogenesis, stimulating ideas about reprogramming, cell fate control, potential mechanisms underpinning developmental plasticity in embryos. In this review, we discuss these regulation importance for safeguarding development. We also highlight unanswered questions key challenges remain to be addressed.

Язык: Английский

Процитировано

45
Comparative proteomic landscapes elucidate human preimplantation development and failure DOI
Wencheng Zhu, Juan Meng, Yán Li

и другие.

Cell, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

8
Dynamic reprogramming of H3K9me3 at hominoid-specific retrotransposons during human preimplantation development DOI Creative Commons

Hanwen Yu,

Manqi Chen,

Yuanlang Hu

и другие.

Cell stem cell, Год журнала: 2022, Номер 29(7), С. 1031 - 1050.e12

Опубликована: Июль 1, 2022

Язык: Английский

Процитировано

65
MYC function and regulation in physiological perspective DOI Creative Commons
Rajeev Jha, Fedor Kouzine, David Levens

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2023, Номер 11

Опубликована: Окт. 24, 2023

MYC, a key member of the Myc-proto-oncogene family, is universal transcription amplifier that regulates almost every physiological process in cell including cycle, proliferation, metabolism, differentiation, and apoptosis. MYC interacts with several cofactors, chromatin modifiers, regulators to direct gene expression. levels are tightly regulated, deregulation has been associated numerous diseases cancer. Understanding comprehensive biology under conditions an utmost necessity demark biological functions from its pathological functions. Here we review recent advances mechanisms, functions, regulation MYC. We also emphasize role as global amplifier.

Язык: Английский

Процитировано

34
Detection of newly synthesized RNA reveals transcriptional reprogramming during ZGA and a role of Obox3 in totipotency acquisition DOI Creative Commons
M. Sakamoto,

Aoi Ito,

Sayaka Wakayama

и другие.

Cell Reports, Год журнала: 2024, Номер 43(4), С. 114118 - 114118

Опубликована: Апрель 1, 2024

Zygotic genome activation (ZGA) after fertilization enables the maternal-to-zygotic transition. However, global view of ZGA, particularly at initiation, is incompletely understood. Here, we develop a method to capture and sequence newly synthesized RNA in early mouse embryos, providing transcriptional reprogramming during ZGA. Our data demonstrate that major ZGA gene begins earlier than previously thought. Furthermore, identify set genes activated minor promoters which show enrichment Obox factor motif, find Obox3 or Obox5 overexpression embryonic stem cells activates genes. Notably, expression factors severely impaired somatic cell nuclear transfer (SCNT) restoration corrects profile greatly improves SCNT embryo development. Hence, our study reveals dynamic underscores crucial role facilitating totipotency acquisition.

Язык: Английский

Процитировано

18
Environmental exposures influence multigenerational epigenetic transmission DOI Creative Commons

Eleanor S. Klibaner-Schiff,

Elisabeth M. Simonin, Cezmi A. Akdiş

и другие.

Clinical Epigenetics, Год журнала: 2024, Номер 16(1)

Опубликована: Окт. 17, 2024

Epigenetic modifications control gene expression and are essential for turning genes on off to regulate maintain differentiated cell types. Epigenetics also modified by a multitude of environmental exposures, including diet pollutants, allowing an individual's environment influence resultant phenotypes clinical outcomes. These epigenetic due gene-environment interactions can be transmitted across generations, raising the possibility that influences occurred in one generation may beyond second generation, exerting long-lasting effect. In this review, we cover known mechanisms modification acquisition, reprogramming persistence, animal models human studies used understand multigenerational transmission, examples environmentally induced change its transmission generations. We highlight importance health not only current population but future generations will experience outcomes through inheritance.

Язык: Английский

Процитировано

11
Placental Dysfunction in Assisted Reproductive Pregnancies: Perinatal, Neonatal and Adult Life Outcomes DOI Open Access
Claudio Manna, Valentina Lacconi, Giuseppe Rizzo

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(2), С. 659 - 659

Опубликована: Янв. 8, 2022

Obstetric and newborn outcomes of assisted reproductive technology (ART) pregnancies are associated with significative prevalence maternal neonatal adverse health conditions, such as cardiovascular metabolic diseases. These data interpreted anomalies in placentation involving a dysregulation several molecular factors pathways. It is not clear which extent the observed placental alterations result ART originate from infertility itself. two aspects probably act synergically for final obstetric risk. Data show that mechanisms inappropriate trophoblast invasion consequent altered vascular remodeling sustain clinical leading to perinatal risks often found pregnancies, preeclampsia, fetal growth restriction placenta previa or accreta. The roles VEGF, GATA3, PIGF, sFLT-1, sEndoglin, EGFL7, melatonin short long embryo cultures, trophectoderm biopsy, cryopreservation, supraphysiologic endometrium preparation, discussed. Inflammatory local conditions epigenetic influence on embryos procedures important research topics since they may have consequences Prevention treatment these represent new frontiers clinicians biologists involved ART, synergic actions researchers at levels advocated.

Язык: Английский

Процитировано

37
The molecular regulatory mechanisms of meiotic arrest and resumption in Oocyte development and maturation DOI Creative Commons
Zhenle Pei, Ke Deng, Congjian Xu

и другие.

Reproductive Biology and Endocrinology, Год журнала: 2023, Номер 21(1)

Опубликована: Окт. 2, 2023

Abstract In human female primordial germ cells, the transition from mitosis to meiosis begins fetal stage. is arrested at diplotene stage of prophase in I (MI) after synapsis and recombination homologous chromosomes, which cannot be segregated. Within follicle, maintenance oocyte meiotic arrest primarily attributed high cytoplasmic concentrations cyclic adenosine monophosphate (cAMP). Depending on specific species, oocytes can remain for extended periods time, ranging months even years. During estrus phase animals or menstrual cycle humans, resumption occurs certain due a surge luteinizing hormone (LH) levels. Any factor interfering with this process may lead impaired maturation, turn affects reproductive function. Nevertheless, precise molecular mechanisms underlying phenomenon has not been systematically summarized yet. To provide comprehensive understanding recently uncovered regulatory network involved development progress cellular nuclear maturation including summarized. Additionally, advancements events occurring oocytes, such as maternal mRNA degradation, posttranslational regulation, organelle distribution associated quality are reviewed. Therefore, pathways regulating will detailed insight into system theoretical basis further research potential approaches novel disease treatments.

Язык: Английский

Процитировано

23