Cellular Signalling, Год журнала: 2023, Номер 108, С. 110727 - 110727
Опубликована: Май 29, 2023
Язык: Английский
Clinical and Translational Medicine, Год журнала: 2025, Номер 15(1)
Опубликована: Янв. 1, 2025
Abstract Alcohol‐related liver disease (ALD) is a common chronic caused by long‐term excessive alcohol consumption and responsible for more than half of all liver‐related deaths worldwide. The molecular mechanisms associated with ALD were not fully understood. In this study, we performed single‐cell RNA sequencing on tissues obtained from patients healthy donors. We identified an ALB + KRT7 epithelial population that expressed both hepatocyte biliary markers significantly expanded in livers. cells demonstrated to have stem cell properties malignant transformation potentials. Moreover, epithelium‐derived organoids promote the tumour growth activating Wnt/β‐catenin signalling cancer cells. Most importantly, blocking Wnt protein secretion or knockdown receptor suppressed promoting effect organoids. Our study provides important insights can be targeted advanced alcohol‐related cirrhosis prevent transformation. addition, our results also uncovered alterations nonparenchymal cells, especially macrophages T/NK populations active inflammation responses hepatitis immunosuppressive microenvironment livers, which likely facilitated progression ALD. Key points This landscape human samples across different stages. epithelium enriched patients, function varied had potential promotion activity. comprehensive changes parenchymal livers lay hidden danger further progression.
Язык: Английский
Процитировано
1
Chemico-Biological Interactions, Год журнала: 2023, Номер 382, С. 110651 - 110651
Опубликована: Июль 28, 2023
Язык: Английский
Процитировано
23
Nature Aging, Год журнала: 2023, Номер 4(1), С. 14 - 26
Опубликована: Дек. 15, 2023
Язык: Английский
Процитировано
20
Hepatology, Год журнала: 2023, Номер 80(1), С. 186 - 201
Опубликована: Ноя. 16, 2023
HCV infection can be successfully managed with antiviral therapies; however, progression to chronic liver disease states, including NAFLD, is common. There currently no reliable in vitro model for investigating host-viral interactions underlying the link between and NAFLD; although organoids (LOs) show promise, they lack nonparenchymal cells, which are key modeling progression.
Язык: Английский
Процитировано
18
Trends in Cell Biology, Год журнала: 2024, Номер 34(10), С. 854 - 864
Опубликована: Фев. 13, 2024
Metazoan organisms are heterocellular systems composed of hundreds different cell types, which arise from an isogenic genome through differentiation. Cellular 'plasticity' further enables cells to alter their fate in response exogenous cues and is involved a variety processes, such as wound healing, infection, cancer. Recent advances cellular model systems, high-dimensional single-cell technologies, lineage tracing have sparked renaissance plasticity research. Here, we discuss the definition plasticity, evaluate state-of-the-art techniques study cell-fate dynamics, explore application technologies obtain functional insights into healthy diseased tissues. The integration advanced biomimetic high-throughput perturbation studies enabling new era research non-genetic metazoan systems.
Язык: Английский
Процитировано
9
Cancer Prevention Research, Год журнала: 2024, Номер 17(4), С. 141 - 155
Опубликована: Янв. 25, 2024
Abstract Inflammation is an essential defense mechanism in which innate immune cells are coordinately activated on encounter of harmful stimuli, including pathogens, tissue injury, and toxic compounds metabolites to neutralize eliminate the instigator initiate healing regeneration. Properly terminated inflammation vital health, but uncontrolled runaway that becomes chronic begets a variety inflammatory metabolic diseases increases cancer risk. Making damaged tissues behave as “wounds do not heal” sustaining production growth factors whose physiologic function healing, accelerates emergence from premalignant lesions. In 1863, Rudolf Virchow, leading German pathologist, suggested possible association between tumor formation, it took another 140 years fully elucidate appreciate tumorigenic role inflammation. Key findings outlined molecular events cascade promote onset progression enabled better appreciation when where should be inhibited. These efforts triggered ongoing research work discover develop inflammation-reducing chemopreventive strategies for decreasing risk incidence.
Язык: Английский
Процитировано
8
Phytotherapy Research, Год журнала: 2024, Номер 38(3), С. 1367 - 1380
Опубликована: Янв. 12, 2024
Abstract Liver fibrosis affects approximately 800 million patients worldwide, with over 2 deaths each year. Nevertheless, there are no approved medications for treating liver fibrosis. In this study, we investigated the impacts of ginkgetin on and underlying mechanisms. The were assessed in mouse models induced by thioacetamide or bile duct ligation. Experiments human LX‐2 cells primary hepatic stellate (HSCs) performed to explore mechanisms, which also validated models. Ginkgetin significantly decreased extracellular matrix deposition HSC activation fibrotic (TAA) ligation (BDL). Beneficial effects existed inhibiting inflammation improving function. vitro experiments showed that markedly inhibited viability apoptosis dose‐dependently. Mechanistic studies revealed antifibrotic depend STAT1 activation, as abolished after silencing vivo fludarabine. Moreover, observed a meaningful cross‐talk between HSCs hepatocytes, IL‐6, released ginkgetin‐induced apoptotic HSCs, enhanced hepatocyte proliferation activating STAT3 signaling. exhibits inducing via enhances secondary IL‐6/STAT3 pathway.
Язык: Английский
Процитировано
7
Journal of Advanced Research, Год журнала: 2023, Номер 60, С. 233 - 248
Опубликована: Авг. 18, 2023
Periodontal regeneration, specifically the restoration of cementum-periodontal ligament (PDL)-alveolar bone complex, remains a formidable challenge in field regenerative dentistry. In light periodontal development, harnessing multi-tissue developmental capabilities cells (PDLCs) and reinitiating process hold great promise as an effective strategy to foster regeneration complex. This study aims delve into potential effects macrophage-mediated immune microenvironment on "developmental engineering" its underlying molecular mechanisms. this study, we conducted comprehensive examination periodontium rat mandibular first molar using histological staining. Through induction diverse microenvironments macrophages, evaluated their re-development events cytokine array. Additionally, investigated PDLC-mediated under these distinct through transcriptome sequencing relevant functional assays. Furthermore, mechanism was also performed. The activation development-related functions PDLCs proved challenging due declined activity. However, our findings suggest that modulating macrophage response can effectively regulate PDLCs-mediated events. M1 type found promote PDLC activities associated with epithelial-mesenchymal transition, fiber degradation, osteoclastogenesis, inflammation Wnt, IL-17, TNF signaling pathways. Conversely, M2 demonstrated superiority inducing epithelium induction, fibers formation, mineralization performance by upregulating TGFβ PI3K-Akt pathway. results could provide some favorable theoretical bases for applying development engineering resolving difficulties regeneration.
Язык: Английский
Процитировано
17
JHEP Reports, Год журнала: 2023, Номер 5(8), С. 100779 - 100779
Опубликована: Апрель 24, 2023
Язык: Английский
Процитировано
14
iScience, Год журнала: 2023, Номер 27(1), С. 108683 - 108683
Опубликована: Дек. 7, 2023
SummaryThe liver possesses a unique regenerative ability to restore its original mass, in this regard, partial hepatectomy (PHx) and transplantation (PLTx) can be executed smoothly safely, which has important implications for the treatment of disease. Liver regeneration (LR) very complicated procedure that involves multiple cytokines transcription factors interact with each other activate different signaling pathways. Activation these pathways drive LR process, divided into three stages, namely, initiation, progression, termination stages. Therefore, it is investigate involved elucidate mechanism LR. This study reviews latest research on key stages LR.Graphical abstract
Язык: Английский
Процитировано
14