Biomaterials Research,
Год журнала:
2024,
Номер
28
Опубликована: Янв. 1, 2024
Human
cerebral
organoids
are
promising
tools
for
investigating
brain
development
and
the
pathogenesis
underlying
neurological
disorders.
To
use
drug
effectiveness
safety
screening,
dispensed
into
each
well
must
be
prepared
under
precisely
same
conditions
as
cells.
Despite
decades
of
extensive
research
on
approaches
to
improve
organoid
generation,
various
challenges
remain,
such
low
yields
heterogeneity
in
size
differentiation
both
within
between
batches.
Here,
we
newly
established
uniform
(UCOs)
derived
from
induced
pluripotent
stem
cells
by
optimizing
performing
real-time
monitoring
telencephalic
marker
expression.
These
exhibited
morphological
uniformity
consistent
expression
FOXG1
during
differentiation,
with
high
productivity.
Moreover,
UCOs
faithfully
recapitulated
early
corticogenesis,
concomitant
establishment
neuroepithelial
populations,
cortical
plate
neurons,
glial
Furthermore,
systematically
developed
neural
networks
excitatory
inhibitory
electrophysiological
signals
when
exposed
neurotransmission
blockers.
Neurodevelopmental
disease
models
manifested
neurite
outgrowth
defects,
which
could
ameliorated
targeted
treatment.
We
propose
an
advanced
platform
variations
reproducibility
modeling
diseases.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3057 - 3057
Опубликована: Март 27, 2025
Tumor
innervation
is
a
complex
interaction
between
nerves
and
cancer
cells
that
consists
of
axons
invading
tumors,
its
complexity
remains
largely
unknown
in
humans.
Although
some
retrospective
studies
have
provided
important
insights
into
the
relationship
further
knowledge
required
about
this
biological
process.
Animal
experiments
elucidated
several
molecular
cellular
mechanisms
tumor
innervation;
however,
no
experimental
models
currently
exist
to
study
interactions
human
nerve
cells.
Human
pluripotent
stem
can
differentiate
neurons
for
research
purposes;
use
these
with
unexplored.
Hence,
here
we
analyze
potential
derived
from
unravel
poorly
understood
innervation.
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 7, 2025
Abstract
Pancreatic
β
cell
loss
by
cellular
stress
contributes
to
diabetes
pathogenesis.
Nevertheless,
the
fundamental
mechanism
of
regulation
remains
elusive.
Here,
it
is
found
that
elevated
zinc
transportation
causes
excessive
in
pancreatic
cells
diabetes.
With
gene‐edited
human
embryonic
stem
cell‐derived
(SC‐β
cells)
and
primary
islets,
results
reveal
initiates
integrated
response
(ISR),
ultimately
leads
death.
By
contrary,
genetic
abolishment
shields
from
exacerbated
endoplasmic
reticulum
(ER
stress)
concurrent
ISR.
To
target
with
a
chemical
inhibitor,
an
isogenic
SC‐β
based
drug‐screening
platform
established.
Surprisingly,
independent
its
traditional
role
as
protein
synthesis
inhibitor
at
high‐dose
(10
µ
m
),
low‐dose
(25
n
)
anisomycin
significantly
inhibits
effectively
prevents
loss.
Remarkably,
vivo
administration
mice
demonstrates
protective
effects
on
type
2
induced
high‐fat
diet.
Overall,
identified
crucial
driver
potential
therapeutic
molecule
for
Analytical Chemistry,
Год журнала:
2024,
Номер
96(29), С. 12129 - 12138
Опубликована: Июль 10, 2024
As
organoids
and
organ-on-chip
(OoC)
systems
move
toward
preclinical
clinical
applications,
there
is
an
increased
need
for
method
validation.
Using
a
liquid
chromatography-mass
spectrometry
(LC-MS)-based
approach,
we
developed
measuring
small-molecule
drugs
metabolites
in
the
cell
medium
directly
sampled
from
liver
organoids/OoC
systems.
The
LC-MS
setup
was
coupled
to
automatic
filtration
filter
flush
system
with
online
solid-phase
extraction
(SPE),
allowing
robust
automated
sample
cleanup/analysis.
For
matrix,
rich
in,
e.g.,
protein,
salts,
amino
acids,
no
preinjection
preparation
steps
(protein
precipitation,
SPE,
etc.)
were
necessary.
approach
demonstrated
tolbutamide
its
metabolite,
4-hydroxytolbutamide
(4HT).
validated
analysis
of
media
human
stem
cell-derived
cultured
static
conditions
on
microfluidic
platform
according
Food
Drug
Administration
(FDA)
guidelines
regards
selectivity,
matrix
effects,
accuracy,
precision,
etc.
allows
hundreds
injections
without
replacing
chromatography
hardware.
In
summary,
drug/metabolite
organoids/OoCs
can
be
performed
robustly
minimal
preparation.
Biomaterials Research,
Год журнала:
2024,
Номер
28
Опубликована: Янв. 1, 2024
Human
cerebral
organoids
are
promising
tools
for
investigating
brain
development
and
the
pathogenesis
underlying
neurological
disorders.
To
use
drug
effectiveness
safety
screening,
dispensed
into
each
well
must
be
prepared
under
precisely
same
conditions
as
cells.
Despite
decades
of
extensive
research
on
approaches
to
improve
organoid
generation,
various
challenges
remain,
such
low
yields
heterogeneity
in
size
differentiation
both
within
between
batches.
Here,
we
newly
established
uniform
(UCOs)
derived
from
induced
pluripotent
stem
cells
by
optimizing
performing
real-time
monitoring
telencephalic
marker
expression.
These
exhibited
morphological
uniformity
consistent
expression
FOXG1
during
differentiation,
with
high
productivity.
Moreover,
UCOs
faithfully
recapitulated
early
corticogenesis,
concomitant
establishment
neuroepithelial
populations,
cortical
plate
neurons,
glial
Furthermore,
systematically
developed
neural
networks
excitatory
inhibitory
electrophysiological
signals
when
exposed
neurotransmission
blockers.
Neurodevelopmental
disease
models
manifested
neurite
outgrowth
defects,
which
could
ameliorated
targeted
treatment.
We
propose
an
advanced
platform
variations
reproducibility
modeling
diseases.
