Nature Reviews Bioengineering, Год журнала: 2024, Номер unknown
Опубликована: Окт. 8, 2024
Язык: Английский
Nature Reviews Genetics, Год журнала: 2024, Номер unknown
Опубликована: Авг. 12, 2024
The DNA methylation field has matured from a phase of discovery and genomic characterization to one seeking deeper functional understanding how this modification contributes development, ageing disease. In particular, the past decade seen many exciting mechanistic discoveries that have substantially expanded our appreciation for generic, evolutionarily ancient can be incorporated into robust epigenetic codes. Here, we summarize current distinct landscapes emerge over mammalian lifespan discuss they interact with other regulatory layers support diverse functions. We then review rising interest in alternative patterns found during senescence somatic transition cancer. Alongside advancements single-cell long-read sequencing technologies, collective insights made across these fields offer new opportunities connect biochemical genetic features cell physiology, developmental potential phenotype. Review, Smith et al. describe development within key disease states, as well different methyltransferases interface histone modifications proteins create maintain them.
Язык: Английский
Процитировано
32
Cell, Год журнала: 2024, Номер 187(13), С. 3284 - 3302.e23
Опубликована: Июнь 1, 2024
Язык: Английский
Процитировано
18
Nature Cell Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 9, 2025
Язык: Английский
Процитировано
1
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 664 - 664
Опубликована: Янв. 14, 2025
While 5-fluorouracil (5FU) plays a central role in chemotherapy for colorectal cancer (CRC), resistance to 5FU remains major challenge CRC treatment, and its underlying mechanisms remain unclear. In this study, we investigated the relationship between acquisition, stemness, energy metabolism. Among two cell lines, HT29 cells exhibited glycolytic quiescent properties, while CT26 relied on oxidative phosphorylation (OXPHOS) energy. contrast, 5FU-resistant sublines (HT29R CT26R), developed through continuous exposure low concentrations of 5FU, demonstrated enhanced stemness. This was associated with dominance, proliferation, reduced reactive oxygen species (ROS) production. However, treatment medium-chain fatty acid lauric shifted OXPHOS, reducing increasing ROS levels, inducing death, therefore reversing resistance. These findings suggest that an enhancement stemness reprogramming metabolism play key roles acquiring CRC. reversed resistance, further clinical studies are required.
Язык: Английский
Процитировано
1
Cell stem cell, Год журнала: 2025, Номер unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Stem Cells and Cloning Advances and Applications, Год журнала: 2025, Номер Volume 18, С. 15 - 34
Опубликована: Фев. 1, 2025
The mammalian oocyte is pivotal in reproductive biology, acting as a central hub for cellular reprogramming and stemness. It uniquely contributes half of the zygotic nuclear genome entirety mitochondrial genome, ensuring individual development health. Oocyte-mediated reprogramming, exemplified by transfer, resets somatic cell identity to achieve pluripotency has transformative potential regenerative medicine. This process critical understanding differentiation, improving assisted technologies, advancing cloning stem research. During fertilization, maternal-zygotic transition shifts developmental control from maternal factors activation, establishing totipotency. Oocytes also harbor that guide remodeling, epigenetic modifications, metabolic enabling early embryogenesis. Structures like mitochondria, lipid droplets, cytoplasmic lattices contribute energy production, molecular regulation, organization. Recent insights into components, such ooplasmic nanovesicles endolysosomal vesicular assemblies (ELVAS), highlight their roles maintaining homeostasis, protein synthesis, efficiency. By unraveling mechanisms inherent oocytes, we advance our cloning, therapy, highlighting valuable significance biology
Язык: Английский
Процитировано
1
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Год журнала: 2025, Номер 1872(3), С. 119925 - 119925
Опубликована: Фев. 22, 2025
Язык: Английский
Процитировано
1
Cell, Год журнала: 2024, Номер 187(13), С. 3194 - 3219
Опубликована: Июнь 1, 2024
Developing functional organs from stem cells remains a challenging goal in regenerative medicine. Existing methodologies, such as tissue engineering, bioprinting, and organoids, only offer partial solutions. This perspective focuses on two promising approaches emerging for engineering human cells: cell-based embryo models interspecies organogenesis. Both exploit the premise of guiding to mimic natural development. We begin by summarizing what is known about early development blueprint recapitulating organogenesis both chimeras. The latest advances fields are discussed before highlighting technological knowledge gaps be addressed developing could achieved using approaches. conclude discussing challenges facing modeling outlining future prospects advancing toward generation tissues basic research translational applications.
Язык: Английский
Процитировано
7
Proceedings of the National Academy of Sciences, Год журнала: 2025, Номер 122(11)
Опубликована: Март 11, 2025
Stem cells possess inherent properties of self-renewal and differentiation, thus hold significant promise for regenerating damaged tissues or replacing lost cells. Unless their therapeutic effects are solely mediated by paracrine, transplanted stem need to be highly plastic adapt the host tissue environment differentiate into constituent tissue-specific repair. used in current cell-based therapies either have limited differentiation potential pluripotent but must strictly restricted avoid tumorigenicity risk vivo. Here, we describe derivation human adult high-plasticity cells, which call guide-integrated (giaSCs), from interaction blood-derived guide umbilical cord tissue–derived mesenchymal stromal (UC-MSCs). The a cell population derived peripheral blood adults. Unidirectional transfer through nanotube-like structures granular substances recipient UC-MSCs gave rise giaSCs. Topical application giaSCs full-layer excisional wounds wild-type mice led reconstitution skin tissue. Systemically administered migrated reside mouse small intestinal lipopolysaccharides then differentiated epithelial These transplantation experiments demonstrated that vivo high plasticity. Additional vitro data showed low immunogenicity nontumorigenic. indicate offer promising approach therapy.
Язык: Английский
Процитировано
0
Development, Год журнала: 2025, Номер 152(7)
Опубликована: Апрель 1, 2025
ABSTRACT Pluripotency, the capacity to generate all cells of body, is a defining property transient population epiblast found in pre-, peri- and post-implantation mammalian embryos. As development progresses, undergo dynamic transitions pluripotency states, concurrent with specification extra-embryonic embryonic lineages. Recently, stem cell-based models pre- human have been developed using that capture key properties at different developmental stages. Here, we review early primate development, comparing states vivo cultured pluripotent representative these states. We consider how status starting influences embryo and, turn, what can learn about epiblast. Finally, discuss limitations questions arising from pioneering studies this emerging field.
Язык: Английский
Процитировано
0