Опубликована: Янв. 1, 2024
Язык: Английский
Signal Transduction and Targeted Therapy, Год журнала: 2025, Номер 10(1)
Опубликована: Фев. 8, 2025
Язык: Английский
Процитировано
8
ACS Nano, Год журнала: 2025, Номер unknown
Опубликована: Фев. 14, 2025
Mesenchymal stem cell (MSC) therapy has emerged as a promising alternative approach for treating acute liver failure (ALF) while confronting the shortage of low efficiency and poor engraftment within hostile milieu. In this study, we establish bioactive decellularized extracellular matrix (dECM) platform that incorporates dihydrolipoic acid (DHLA)-protected Pt nanoclusters doped with Cu (PtCu-DHLA) nanozymes cell-laden microgels. The PtCu-DHLA nanozymes, selected their versatility, function antioxidant, anti-inflammatory, pro-proliferative, pro-angiogenic agents, enhancing ALF alleviation providing an optimal microenvironment MSC transplantation. Additionally, methacrylic anhydride (MA)-modified porcine liver-derived (PLdECM) hydrogel (PLdECMMA) been developed construction microgels via microfluidic devices. Interferon γ (IFNγ) preconditioned MSCs encapsulated in PLdECMMA exhibit enhanced immunomodulating activity prolonged survival. are codelivered by leveraging PLdECM orthotopic transplanted dECM enables efficient successful rescue CCl4-induced counteracting oxidative stress, suppressing inflammatory storms, promoting cellular regeneration. Overall, study highlights synergistic reinforced strategy combines biomimetic therapy, offering significant potential treatment broader applications regenerative medicine.
Язык: Английский
Процитировано
2
International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 135565 - 135565
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
6
Nature Communications, Год журнала: 2025, Номер 16(1)
Опубликована: Фев. 16, 2025
Deciphering the features, structure, and functions of cell niche in tissues remains a major challenge. Here, we present scNiche, computational framework to identify characterize niches from spatial omics data at single-cell resolution. We benchmark scNiche with both simulated biological datasets, demonstrate that can effectively robustly while outperforming other existing methods. In proteomics human triple-negative breast cancer, reveals influence microenvironment on cellular phenotypes, further dissects patient-specific distinct compositions or phenotypic characteristics. By analyzing mouse liver transcriptomics across normal early-onset failure donors, uncovers disease-specific injury niches, delineates remodeling failure. Overall, enables decoding data. authors develop characterise
Язык: Английский
Процитировано
0
Expert Review of Gastroenterology & Hepatology, Год журнала: 2025, Номер unknown
Опубликована: Май 19, 2025
The liver is a central metabolic organ, but also hosting unique immune microenvironment to sustain homeostasis and proper defense measures against injury threats in healthy individuals. Liver macrophages, mostly represented by the tissue-resident Kupffer cells bone marrow- or monocyte-derived are intricately involved various aspects of disease, including tissue injury, inflammation, fibrogenesis repair mechanisms. We review recent findings on defining macrophage landscape their functions diseases with aim highlighting potential targets for therapeutic interventions. A comprehensive literature search PubMed Google Scholar was conducted identify relevant up date. macrophages orchestrate key homeostatic pathogenic processes liver. Thus, targeting represents an attractive strategy drug development, e.g. ameliorate steatohepatitis fibrosis. However, translation from fundamental research therapies remains challenging due versatile nature compartment. Recent major technical advances such as single-cell spatially-resolved omics approaches deepened our understanding biology at molecular level. Yet, further studies needed suitable, etiology- stage-dependent strategies treatment diseases.
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2024, Номер 96(49), С. 19370 - 19377
Опубликована: Ноя. 27, 2024
Idiosyncratic drug-induced liver injury (iDILI) poses significant challenges in both drug development and clinical practice due to its unpredictable nature poorly understood mechanism. The current
Язык: Английский
Процитировано
2
Life Metabolism, Год журнала: 2024, Номер 3(6)
Опубликована: Май 30, 2024
Metabolic dysfunction-associated steatotic liver disease (MASLD) is a metabolic that can progress to steatohepatitis (MASH), cirrhosis, and cancer. The zonal distribution of biomolecules in the implicated mediating progression. Recently, G-protein-coupled receptor 35 (GPR35) has been highlighted play role MASLD, but precise mechanism not fully understood, particularly, liver-zonal manner. Here, we aimed identify spatially distributed specific genes metabolites different zonation are regulated by GPR35 combining lipid metabolomics, spatial transcriptomics (ST), metabolomics (SM). We found influenced accumulation, inflammatory metabolism-related factors regions, notably affecting anti-inflammation factor ELF4 (E74 like E-twenty six (ETS) transcription 4), homeostasis key CIDEA (cell death-inducing DNA fragmentation alpha (DFFA)-like effector A), injury response-related SAA1/2/3 (serum amyloid A1/2/3), thereby impacting MASLD Furthermore, SM elucidated metabolite distributions across such as C10H11N4O7P (3',5'-cyclic inosine monophosphate (3',5'-IMP)) for central vein, this significantly decreased zones GPR35-deficient mice during Taken together, regulates hepatocyte damage repair, controls inflammation, prevents progression influencing phospholipid gene expression
Язык: Английский
Процитировано
1
Cellular and Molecular Gastroenterology and Hepatology, Год журнала: 2024, Номер 18(6), С. 101395 - 101395
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
1
Cell Reports, Год журнала: 2024, Номер 44(1), С. 115093 - 115093
Опубликована: Дек. 24, 2024
Язык: Английский
Процитировано
1
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0