bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 29, 2023
Abstract
Synthetic
developmental
biology
aims
to
engineer
gene
regulatory
mechanisms
(GRMs)
for
understanding
and
producing
desired
multicellular
patterns
shapes.
However,
designing
GRMs
spatial
is
a
current
challenge
due
the
nonlinear
interactions
feedback
loops
in
genetic
circuits.
Here
we
present
methodology
automatically
design
that
can
produce
any
given
pattern.
The
proposed
approach
uses
two
orthogonal
morphogen
gradients
acting
as
positional
information
signals
tissue
area
or
culture,
which
constitutes
continuous
field
of
engineered
cells
implementing
same
designed
GRM.
To
efficiently
both
circuit
network
interaction
mechanisms—including
number
genes
necessary
formation
target
pattern—we
developed
an
automated
algorithm
based
on
high-performance
evolutionary
computation.
tolerance
be
configured
are
either
simple
approximate
complex
precise
patterns.
We
demonstrate
by
diverse
set
synthetic
expression
interpreting
just
gradients.
framework
offers
versatile
systematically
discover
pattern-producing
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(21)
Опубликована: Янв. 22, 2024
Brain
organoids
are
3D
in
vitro
culture
systems
derived
from
human
pluripotent
stem
cells
that
self-organize
to
model
features
of
the
(developing)
brain.
This
review
examines
techniques
behind
organoid
generation,
their
current
and
potential
applications,
future
directions
for
field.
possess
complex
architecture
containing
various
neural
cell
types,
synapses,
myelination.
They
have
been
utilized
toxicology
testing,
disease
modeling,
infection
studies,
personalized
medicine,
gene-environment
interaction
studies.
An
emerging
concept
termed
Organoid
Intelligence
(OI)
combines
with
artificial
intelligence
generate
learning
memory,
goals
modeling
cognition
enabling
biological
computing
applications.
allow
neuroscience
studies
not
previously
achievable
traditional
techniques,
transform
drug
development,
understanding
brain
development
disorders.
The
aspirational
vision
OI
parallels
origins
intelligence,
efforts
underway
map
a
roadmap
toward
its
realization.
In
summary,
constitute
disruptive
technology
is
rapidly
advancing
gaining
traction
across
multiple
disciplines.
Environment International,
Год журнала:
2023,
Номер
172, С. 107801 - 107801
Опубликована: Фев. 1, 2023
Atmospheric
pollutants,
including
particulate
matters,
nanoparticles,
bioaerosols,
and
some
chemicals,
have
posed
serious
threats
to
the
environment
human's
health.
The
lungs
are
responsible
organs
for
providing
interface
between
circulatory
system
external
environment,
where
pollutant
particles
can
deposit
or
penetrate
into
bloodstream
circulation.
Conventional
studies
decipher
mechanism
underlying
air
pollution
human
health
quite
limited,
due
lack
of
reliable
models
that
reproduce
in
vivo
features
lung
tissues
after
pollutants
exposure.
In
past
decade,
advanced
near-to-native
chips,
combining
cell
biology
with
bioengineered
technology,
present
a
new
strategy
atmospheric
assessment
narrow
gap
2D
culture
animal
models.
this
review,
key
artificial
chips
cutting-edge
technologies
chip
manufacture
introduced.
recent
progresses
exposure
summarized
highlighted.
We
further
discuss
current
challenges
future
opportunities
development
their
potential
utilities
associated
toxicity
testing
drug
screening.
Science,
Год журнала:
2022,
Номер
378(6622), С. 864 - 868
Опубликована: Ноя. 24, 2022
The
application
of
synthetic
biology
approaches
to
study
development
opens
the
possibility
build
and
manipulate
developmental
processes
understand
them
better.
Researchers
have
reconstituted
fundamental
processes,
such
as
cell
patterning
sorting,
by
engineering
gene
circuits
in
vitro.
Moreover,
new
tools
been
created
that
allow
for
control
more
complex
organoids
embryos.
Synthetic
testing
which
components
are
sufficient
reproduce
a
process
under
conditions
well
what
effect
perturbations
on
other
processes.
We
envision
future
requires
an
increase
diversity
available
further
efforts
combine
multiple
into
one
system.
Journal of Experimental Botany,
Год журнала:
2023,
Номер
74(13), С. 3787 - 3790
Опубликована: Июль 18, 2023
Plant
synthetic
biology
allows
for
the
reprogramming
of
biological
systems
to
promote
sustainable
agriculture.It
has
a
range
applications,
including
improving
nutritional
quality,
engineering
resilient
crops,
creating
new
materials,
and
producing
various
bioproducts.In
addition,
plant
provides
way
answer
fundamental
questions
in
biology.This
Special
Issue
presents
collection
review
articles
that
delve
into
current
future
state
biology.
Seminars in Cell and Developmental Biology,
Год журнала:
2023,
Номер
147, С. 83 - 90
Опубликована: Фев. 6, 2023
Understanding
the
mechanism
by
which
cells
coordinate
their
differentiation
and
migration
is
critical
to
our
understanding
of
many
fundamental
processes
such
as
wound
healing,
disease
progression,
developmental
biology.
Mathematical
models
have
been
an
essential
tool
for
testing
developing
understanding,
soft
spherical
particles,
reaction-diffusion
systems
that
couple
cell
movement
environmental
factors,
multi-scale
multi-physics
simulations
combine
bottom-up
rule-based
with
continuum
laws.
However,
mathematical
can
often
be
loosely
related
data
or
so
parameters
model
behaviour
weakly
constrained.
Recent
methods
in
machine
learning
introduce
new
means
derived
deployed.
In
this
review,
we
discuss
examples
aspects
biology,
migration,
how
these
combined
recent
methods.
Journal of Experimental Botany,
Год журнала:
2023,
Номер
74(13), С. 3864 - 3876
Опубликована: Май 8, 2023
Abstract
Plant
morphology
and
anatomy
strongly
influence
agricultural
yield.
Crop
domestication
has
strived
for
desirable
growth
developmental
traits,
such
as
larger
more
fruits
semi-dwarf
architecture.
Genetic
engineering
accelerated
rational,
purpose-driven
of
plant
development,
but
it
can
be
unpredictable.
Developmental
pathways
are
complex
riddled
with
environmental
hormonal
inputs,
well
feedback
feedforward
interactions,
which
occur
at
specific
times
places
in
a
growing
multicellular
organism.
Rational
modification
development
would
probably
benefit
from
precision
based
on
synthetic
biology
approaches.
This
review
outlines
recently
developed
technologies
systems
highlights
their
potential
development.
Streamlined
high-capacity
genetic
construction
methods
(Golden
Gate
DNA
Assembly
frameworks
toolkits)
allow
fast
variation-series
cloning
multigene
transgene
constructs.
This,
together
suite
gene
regulation
tools
(e.g.
cell
type-specific
promoters,
logic
gates,
multiplex
systems),
is
starting
to
enable
pathway
predictable
outcomes
model
crop
species.
Quantitative Biology,
Год журнала:
2025,
Номер
13(4)
Опубликована: Апрель 17, 2025
Abstract
Bacterial
colonies,
as
dynamic
ecosystems,
display
intricate
behaviors
and
organizational
structures
that
profoundly
influence
their
survival
functionality.
These
communities
engage
in
physiological
social
interactions,
resulting
remarkable
spatial
heterogeneity.
Recent
advancements
technology
modeling
have
significantly
enhanced
our
comprehension
of
these
phenomena,
shedding
light
on
the
underlying
mechanisms
governing
bacterial
colony
development.
In
this
review,
we
explore
multifaceted
aspects
emphasizing
intricacies,
innovative
research
tools,
predictive
approaches.
By
integrating
diverse
perspectives,
aim
to
deepen
understanding
microbial
pave
way
for
novel
applications
biotechnology,
ecology,
medicine.
