Design and Mathematical Analysis of Synthetic Inhibitory Circuits that Program Self-Organizing Multicellular Structures DOI Creative Commons
Calvin Lam

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 18, 2023

ABSTRACT Bottom-up approaches are becoming increasingly popular for studying multicellular self-organization and development. In contrast to the classic top-down approach, where parts of organization/developmental process broken understand process, goal is build it. For example, synthetic circuits have been built how cell-cell communication differential adhesion can drive The majority current bottom-up efforts focus on using activatory engineer development, but with inhibitory minimal. Yet, ubiquitous vital native developmental processes. Thus, a crucial yet poorly studied facet To demonstrate potential building developing structures, I designed several that combine engineered adhesion. Using previously validated in silico framework, examine capability these show variety patterned, self-organized structures even morphological oscillations. These results support be powerful tools building, studying, understanding Graphical Abstract

Язык: Английский

The Promise and Potential of Brain Organoids DOI Creative Commons
Lena Smirnova, Thomas Härtung

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(21)

Опубликована: Янв. 22, 2024

Brain organoids are 3D in vitro culture systems derived from human pluripotent stem cells that self-organize to model features of the (developing) brain. This review examines techniques behind organoid generation, their current and potential applications, future directions for field. possess complex architecture containing various neural cell types, synapses, myelination. They have been utilized toxicology testing, disease modeling, infection studies, personalized medicine, gene-environment interaction studies. An emerging concept termed Organoid Intelligence (OI) combines with artificial intelligence generate learning memory, goals modeling cognition enabling biological computing applications. allow neuroscience studies not previously achievable traditional techniques, transform drug development, understanding brain development disorders. The aspirational vision OI parallels origins intelligence, efforts underway map a roadmap toward its realization. In summary, constitute disruptive technology is rapidly advancing gaining traction across multiple disciplines.

Язык: Английский

Процитировано

25

Advances of microfluidic lung chips for assessing atmospheric pollutants exposure DOI Creative Commons
Hui Wang,

Fangchao Yin,

Zhongyu Li

и другие.

Environment International, Год журнала: 2023, Номер 172, С. 107801 - 107801

Опубликована: Фев. 1, 2023

Atmospheric pollutants, including particulate matters, nanoparticles, bioaerosols, and some chemicals, have posed serious threats to the environment human's health. The lungs are responsible organs for providing interface between circulatory system external environment, where pollutant particles can deposit or penetrate into bloodstream circulation. Conventional studies decipher mechanism underlying air pollution human health quite limited, due lack of reliable models that reproduce in vivo features lung tissues after pollutants exposure. In past decade, advanced near-to-native chips, combining cell biology with bioengineered technology, present a new strategy atmospheric assessment narrow gap 2D culture animal models. this review, key artificial chips cutting-edge technologies chip manufacture introduced. recent progresses exposure summarized highlighted. We further discuss current challenges future opportunities development their potential utilities associated toxicity testing drug screening.

Язык: Английский

Процитировано

21

Organoids from pluripotent stem cells and human tissues: When two cultures meet each other DOI Creative Commons
Benedetta Artegiani, Delilah Hendriks

Developmental Cell, Год журнала: 2025, Номер 60(4), С. 493 - 511

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1

Scaling up complexity in synthetic developmental biology DOI
Guillermo Martínez-Ara, Kristina S. Stapornwongkul, Miki Ebisuya

и другие.

Science, Год журнала: 2022, Номер 378(6622), С. 864 - 868

Опубликована: Ноя. 24, 2022

The application of synthetic biology approaches to study development opens the possibility build and manipulate developmental processes understand them better. Researchers have reconstituted fundamental processes, such as cell patterning sorting, by engineering gene circuits in vitro. Moreover, new tools been created that allow for control more complex organoids embryos. Synthetic testing which components are sufficient reproduce a process under conditions well what effect perturbations on other processes. We envision future requires an increase diversity available further efforts combine multiple into one system.

Язык: Английский

Процитировано

22

Plant synthetic biology: exploring the frontiers of sustainable agriculture and fundamental plant biology DOI Open Access
Jae‐Seong Yang, Ivan Reyna‐Llorens

Journal of Experimental Botany, Год журнала: 2023, Номер 74(13), С. 3787 - 3790

Опубликована: Июль 18, 2023

Plant synthetic biology allows for the reprogramming of biological systems to promote sustainable agriculture.It has a range applications, including improving nutritional quality, engineering resilient crops, creating new materials, and producing various bioproducts.In addition, plant provides way answer fundamental questions in biology.This Special Issue presents collection review articles that delve into current future state biology.

Язык: Английский

Процитировано

13

Generative models of morphogenesis in developmental biology DOI Creative Commons
Namid Stillman, Roberto Mayor

Seminars in Cell and Developmental Biology, Год журнала: 2023, Номер 147, С. 83 - 90

Опубликована: Фев. 6, 2023

Understanding the mechanism by which cells coordinate their differentiation and migration is critical to our understanding of many fundamental processes such as wound healing, disease progression, developmental biology. Mathematical models have been an essential tool for testing developing understanding, soft spherical particles, reaction-diffusion systems that couple cell movement environmental factors, multi-scale multi-physics simulations combine bottom-up rule-based with continuum laws. However, mathematical can often be loosely related data or so parameters model behaviour weakly constrained. Recent methods in machine learning introduce new means derived deployed. In this review, we discuss examples aspects biology, migration, how these combined recent methods.

Язык: Английский

Процитировано

11

Synthetic developmental biology: molecular tools to re-design plant shoots and roots DOI Creative Commons
Elif Gediz Kocaoglan, Dhanya Radhakrishnan, Naomi Nakayama

и другие.

Journal of Experimental Botany, Год журнала: 2023, Номер 74(13), С. 3864 - 3876

Опубликована: Май 8, 2023

Abstract Plant morphology and anatomy strongly influence agricultural yield. Crop domestication has strived for desirable growth developmental traits, such as larger more fruits semi-dwarf architecture. Genetic engineering accelerated rational, purpose-driven of plant development, but it can be unpredictable. Developmental pathways are complex riddled with environmental hormonal inputs, well feedback feedforward interactions, which occur at specific times places in a growing multicellular organism. Rational modification development would probably benefit from precision based on synthetic biology approaches. This review outlines recently developed technologies systems highlights their potential development. Streamlined high-capacity genetic construction methods (Golden Gate DNA Assembly frameworks toolkits) allow fast variation-series cloning multigene transgene constructs. This, together suite gene regulation tools (e.g. cell type-specific promoters, logic gates, multiplex systems), is starting to enable pathway predictable outcomes model crop species.

Язык: Английский

Процитировано

11

Understanding the development of bacterial colony: Physiology, new technology, and modeling DOI Open Access
Jingwen Zhu, Pan Chu, Xiongfei Fu

и другие.

Quantitative Biology, Год журнала: 2025, Номер 13(4)

Опубликована: Апрель 17, 2025

Abstract Bacterial colonies, as dynamic ecosystems, display intricate behaviors and organizational structures that profoundly influence their survival functionality. These communities engage in physiological social interactions, resulting remarkable spatial heterogeneity. Recent advancements technology modeling have significantly enhanced our comprehension of these phenomena, shedding light on the underlying mechanisms governing bacterial colony development. In this review, we explore multifaceted aspects emphasizing intricacies, innovative research tools, predictive approaches. By integrating diverse perspectives, aim to deepen understanding microbial pave way for novel applications biotechnology, ecology, medicine.

Язык: Английский

Процитировано

0

Engineering plant photoreceptors towards enhancing plant productivity DOI

Ramyani Bhattacharjee,

Highland Kayang, Eros Kharshiing

и другие.

Plant Molecular Biology, Год журнала: 2025, Номер 115(3)

Опубликована: Май 6, 2025

Язык: Английский

Процитировано

0

Synthetic symmetry breaking and programmable multicellular structure formation DOI Creative Commons
Noreen Wauford,

Akshay M. Patel,

Jesse Tordoff

и другие.

Cell Systems, Год журнала: 2023, Номер 14(9), С. 806 - 818.e5

Опубликована: Сен. 1, 2023

Язык: Английский

Процитировано

8