Recent
germline
stem
cell
technology
advancements
paved
the
foundation
for
treating
infertility
and
making
amenable
progress
in
resurrection
of
germplasm.
Globally
countless
issues
are
being
accounted
with
germplasm
quality
associated
male
a
decline
sexual
desire.
The
improvement
research
on
macaques
very
close
to
humans
may
translate
regenerative
medicine
aspects
treatment.
Induced
pluripotent
cells
bio-design
now
it
possible
resurrect
germ
by
relying
conducive
tissue
environment,
maturation;
improvised
microenvironment
modulations
monitoring
hormonal
regimen
cyclic
dynamics
maturation
factors
lead
differentiation
deafferentation
approaches.
There
is
tremendous
scope
mice
xenograft
model
vitro
trans-differentiation
technologies
emerging
as
future
hope
preserved
cancer
patients
who
have
undergone
chemo-
radiotherapy
besides
renewal
wildlife
improved
breeding
endangered
animal
species
wild.
Reproduction in Domestic Animals,
Год журнала:
2024,
Номер
59(5)
Опубликована: Май 1, 2024
Meat
and
eggs
from
chicken
are
the
major
source
of
animal
protein
for
human
population.
The
cryopreservation
poultry
species
is
needed
to
guarantee
sustainable
production.
Here,
we
describe
existing
technologies
avian
reproductive
cells
using
embryonic
germ
cells,
spermatozoa
ovarian
tissues.
We
outline
strategies
reconstitute
breeds
their
cryopreserved
surrogate
hosts
discuss
perspectives
genetic
conservation
reconstitution
wild
host
animals.
Journal of Clinical Investigation,
Год журнала:
2024,
Номер
134(12)
Опубликована: Май 7, 2024
Satellite
cells,
the
stem
cells
of
skeletal
muscle
tissue,
hold
a
remarkable
regeneration
capacity
and
therapeutic
potential
in
regenerative
medicine.
However,
low
satellite
cell
yield
from
autologous
or
donor-derived
muscles
hinders
adoption
transplantation
for
treatment
diseases,
including
Duchenne
muscular
dystrophy
(DMD).
To
address
this
limitation,
here
we
investigated
whether
can
be
derived
allogeneic
xenogeneic
animal
hosts.
First,
injection
CRISPR/Cas9-corrected
mouse
DMD-induced
pluripotent
(iPSCs)
into
blastocysts
carrying
an
ablation
system
host
gave
rise
to
intraspecies
chimeras
exclusively
iPSC-derived
cells.
Furthermore,
genetically
corrected
DMD-iPSCs
rat
resulted
formation
interspecies
rat-mouse
harboring
Remarkably,
derivative
myoblasts
produced
restored
dystrophin
expression
DMD
mice
following
intramuscular
transplantation,
contributed
pool.
Collectively,
study
demonstrates
feasibility
producing
therapeutically
competent
across
divergent
species,
raising
possibility
generating
human
large
animals
medicine
purposes.
animal,
Год журнала:
2023,
Номер
17, С. 100803 - 100803
Опубликована: Май 1, 2023
The
progress
made
in
recent
years
the
derivation
and
culture
of
pluripotent
stem
cells
from
farm
animals
opens
up
possibility
creating
livestock
chimeras.
Chimeras
producing
gametes
exclusively
derived
elite
donor
could
pass
superior
genetics
on
to
next
generation
thereby
reduce
genetic
lag
that
typically
exists
between
breeding
sector
commercial
production
sector,
especially
for
industries
like
beef
sheep
where
is
commonly
disseminated
through
natural
service
mating.
carrying
germ
generated
genome-edited
or
genetically
engineered
further
disseminate
useful
genomic
alterations
such
as
climate
adaptation,
animal
welfare
improvements,
repair
deleterious
conditions,
and/or
elimination
undesired
traits
disease
susceptibility
generation.
Despite
successful
chimeras
with
injected
into
preimplantation-stage
blastocysts
model
species,
there
are
no
documented
cases
this
occurring
livestock.
Here,
we
review
literature
ungulates,
chimeric
ungulate
agricultural
applications,
drawing
insights
studies
done
discuss
future
possibilities
fast-moving
developing
field.
Aside
technical
aspects,
consistency
regulatory
approach
taken
by
different
jurisdictions
towards
their
progeny
will
be
an
important
determinant
industry
uptake
adoption
agriculture.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 13, 2023
SUMMARY
Interspecies
organogenesis
via
blastocyst
complementation
provides
a
unique
platform
to
study
development
in
an
evolutionarily
context
and
holds
potential
overcome
world-wide
organ
shortages
1
.
By
using
this
technique,
rat
pancreas,
thymus,
heart,
eye
tissues
have
been
generated
mice
2–4
To
date,
however,
xeno-generation
of
brain
has
not
achieved
through
complementation.
Here,
we
developed
optimized
one-step
strategy
based
on
C-CRISPR
5
,
which
facilitated
rapid
screening
candidate
genes
support
Among
the
seven
WNT
pathway-related
selected
for
targeting,
only
Dkk1
or
Hesx1
deficiency
supported
forebrain
by
injection
mouse
embryonic
stem
cells
(mESCs).
Further,
injecting
ESCs
(rESCs)
into
blastocysts
deficient
but
adult
chimeric
forebrains
comprised
large
proportion
that
were
structurally
functionally
similar
forebrains.
Our
analysis
revealed
rESC-derived
along
spatial-temporal
trajectory
with
rather
than
forebrains,
gene
expression
profiles
nerve
surprisingly
maintained
characteristics
cells.
We
noted
rate
gradually
decreased
as
progressed,
suggesting
xenogeneic
barriers
during
mid-to-late
prenatal
development.
opens
door
studying
conserved
divergent
mechanisms
underlying
cognitive
function.
The
C-CRIPSR
IBC
here
great
broaden
application
interspecies
organogenesis.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 12, 2023
Abstract
Satellite
cells,
the
stem
cells
of
skeletal
muscle
tissue,
hold
a
prodigious
regeneration
capacity.
However,
low
satellite
cell
yield
from
autologous
or
donor-derived
muscles
precludes
adoption
transplantation
for
treatment
diseases
including
Duchenne
muscular
dystrophy
(DMD).
To
address
this
limitation,
here
we
investigated
whether
sufficient
quantity
can
be
produced
in
allogeneic
xenogeneic
animal
hosts.
First,
report
on
exclusive
production
intraspecies
mouse
chimeras
by
injection
CRISPR/Cas9-corrected
DMD-induced
pluripotent
(iPSCs)
into
blastocysts
carrying
an
ablation
system
host
Pax7+
cells.
Additionally,
genetically-corrected
DMD-iPSCs
rat
interspecies
rat-mouse
harboring
that
efficiently
restored
dystrophin
expression
DMD
mice.
This
study
thus
provides
proof-of-principle
generation
therapeutically-competent
between
divergent
species,
raising
possibility
procuring
human
large
animals
regenerative
medicine
purposes.
