Early cell cycle genes in cortical organoid progenitors predict interindividual variability in infant brain growth trajectories DOI Creative Commons

Madison R. Glass,

Nana Matoba, Alvaro A. Beltran

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 8, 2025

Summary Human induced pluripotent stem cell (iPSC) derived cortical organoids (hCOs) model neurogenesis on an individual’s genetic background. The degree to which hCO phenotypes recapitulate the brain growth of participants from they were is not well established. We generated up 3 iPSC clones each 18 in Infant Brain Imaging Study, who have undergone longitudinal imaging during infancy. identified consistent morphology and types across same participant. cross-sectional area production hem cells associated with vivo rates. Cell cycle genes expression early progenitors at crux fate decision trajectories correlated rate 6-12 months age, enriched microcephaly neurodevelopmental disorder genes. Our data suggest hCOs capture inter-individual variation influencing infant surface expansion.

Язык: Английский

Poly(2-alkyl-2-oxazoline) Hydrogels as Synthetic Matrices for Multicellular Spheroid and Intestinal Organoid Cultures DOI

Robin Vanhoeijen,

Irina A. Okkelman,

Nette Rogier

и другие.

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

The extracellular matrix (ECM) plays a crucial role in organoid cultures by supporting cell proliferation and differentiation. A key feature of the ECM is its mechanical influence on surrounding cells, directly affecting their behavior. Matrigel, most commonly used ECM, limited animal-derived origin, batch variability, uncontrollable properties, restricting use 3D cell-model-based mechanobiological studies. Poly(2-alkyl-2-oxazoline) (PAOx) synthetic hydrogels represent an appealing alternative because reproducibility versatile chemistry, enabling tuning hydrogel stiffness functionalization. Here, we studied PAOx with differing compressive moduli for potential to support growth. spheroid growth over several days without addition components. Furthermore, discovered intestinal epithelial polarity reversion demonstrate how tunable properties can be study effects morphology oxygenation live multicellular spheroids.

Язык: Английский

Процитировано

0
Early cell cycle genes in cortical organoid progenitors predict interindividual variability in infant brain growth trajectories DOI Creative Commons

Madison R. Glass,

Nana Matoba, Alvaro A. Beltran

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 8, 2025

Summary Human induced pluripotent stem cell (iPSC) derived cortical organoids (hCOs) model neurogenesis on an individual’s genetic background. The degree to which hCO phenotypes recapitulate the brain growth of participants from they were is not well established. We generated up 3 iPSC clones each 18 in Infant Brain Imaging Study, who have undergone longitudinal imaging during infancy. identified consistent morphology and types across same participant. cross-sectional area production hem cells associated with vivo rates. Cell cycle genes expression early progenitors at crux fate decision trajectories correlated rate 6-12 months age, enriched microcephaly neurodevelopmental disorder genes. Our data suggest hCOs capture inter-individual variation influencing infant surface expansion.

Язык: Английский

Процитировано

0