Physiologia Plantarum,
Год журнала:
2024,
Номер
176(5)
Опубликована: Сен. 1, 2024
Abstract
Pathogenesis‐related
proteins
(PR),
including
osmotins,
play
a
vital
role
in
plant
defense,
being
activated
response
to
diverse
biotic
and
abiotic
stresses.
Despite
their
significance,
the
mechanistic
insights
into
of
osmotins
defense
have
not
been
extensively
explored.
The
present
study
explores
cloning
characterization
osmotin
gene
(
WsOsm
)
from
Withania
somnifera
,
aiming
illuminate
its
mechanisms.
Quantitative
real‐time
PCR
analysis
revealed
significant
induction
various
phytohormones
e.g.
abscisic
acid,
salicylic
methyl
jasmonate,
brassinosteroids,
ethrel,
as
well
stresses
like
heat,
cold,
salt,
drought.
To
further
elucidate
's
functional
role,
we
overexpressed
Nicotiana
tabacum
resulting
heightened
resistance
against
Alternaria
solani
pathogen.
Additionally,
observed
enhancements
shoot
length,
root
biomass
transgenic
tobacco
plants
compared
wild
plants.
Notably,
WsOsm‐
overexpressing
seedlings
demonstrated
improved
salt
drought
stress
tolerance,
particularly
at
seedling
stage.
Confocal
histological
H
2
O
biochemical
studies
antioxidant
enzyme
activities
higher
levels
lines,
indicating
enhanced
defense.
Furthermore,
pull‐down
assay
mass
spectrometry
potential
interaction
between
defensin,
known
antifungal
PR
protein
(WsDF).
This
suggests
novel
mediating
responses
by
interacting
with
other
proteins.
Overall,
these
findings
pave
way
for
future
applications
developing
stress‐tolerant
crops
improving
strategies
pathogens.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(9), С. 3944 - 3944
Опубликована: Апрель 22, 2025
Cotton
is
a
crucial
cash
crop
widely
valued
for
its
fiber.
It
an
important
source
of
natural
fiber
and
has
diverse
applications.
Improving
quality
significant
economic
agricultural
importance.
Purple
acid
phosphatases
(PAPs)
are
multifunctional
enzymes
critical
plant
cell
wall
biosynthesis,
root
architecture
modulation,
low-phosphorus
stress
adaptation,
salt/ROS
tolerance.
In
this
study,
comprehensive
genome-wide
analysis
the
PAP
gene
family
was
performed
four
cotton
species
(G.
hirsutum,
G.
barbadense,
raimondii,
arboreum)
to
explore
potential
role
in
improving
quality.
A
total
193
genes
were
identified
these
species,
revealing
several
conserved
domains
that
contribute
their
functional
diversity.
Phylogenetic
showed
PAP2
exhibited
high
homology
with
NtPAP12,
synthesis-related
gene.
Using
varieties
contrasting
thickness
(EZ60,
micronaire
4.5
vs.
CCRI127,
3.5),
qRT-PCR
demonstrated
significantly
higher
expression
levels
GhPAP2.2,
GhPAP2.6,
GhPAP2.8,
GhPAP2.9
EZ60
fibers
during
20–25
DPA
compared
CCRI127.
These
results
highlight
influence
on
development
provide
valuable
insights
breeding.
Abscisic
acid
(ABA)
plays
a
central
role
in
regulating
plant
responses
to
abiotic
stress.
It
orchestrates
complex
regulatory
network
that
facilitates
the
transition
from
growth
defense.
Understanding
molecular
mechanisms
underlying
this
ABA-induced
defense
is
essential
for
elucidating
adaptive
strategies
under
environmental
stress
conditions.
In
study,
we
used
refined
dynamic
biomarker
(DNB)
approach
quantitatively
identify
critical
signal
(CTS)
and
characterize
regulated
stochasticity
during
Arabidopsis
thaliana.
By
integrating
high-resolution
time-series
RNA-seq
data
with
analysis,
identified
set
of
DNB
genes
serve
as
key
regulators
transition.
The
phase
was
precisely
at
ninth
time
point
(6
h
after
treatment),
which
marks
crucial
switch
growth-dominated
-oriented
state.
Gene
Ontology
(GO)
enrichment
analysis
revealed
significant
overrepresentation
defense-related
biological
processes,
while
STRING
strong
functional
interactions
between
differentially
expressed
(DEGs)
highlighted
hubs.
particular,
hub
such
PIF4,
TPS8,
NIA1,
HSP90-5
were
potential
master
ABA-mediated
activation,
highlighting
their
importance
adaptation.
network-driven
transcriptomic
study
provides
new
insights
into
basis
transitions
identification
CTS
perspective
on
conceptual
framework
improving
crop
resistance.
addition,
establishment
comprehensive
database
ABA-responsive
represents
valuable
resource
future
research
adaptation
resilience.
Physiologia Plantarum,
Год журнала:
2024,
Номер
176(5)
Опубликована: Сен. 1, 2024
Abstract
Pathogenesis‐related
proteins
(PR),
including
osmotins,
play
a
vital
role
in
plant
defense,
being
activated
response
to
diverse
biotic
and
abiotic
stresses.
Despite
their
significance,
the
mechanistic
insights
into
of
osmotins
defense
have
not
been
extensively
explored.
The
present
study
explores
cloning
characterization
osmotin
gene
(
WsOsm
)
from
Withania
somnifera
,
aiming
illuminate
its
mechanisms.
Quantitative
real‐time
PCR
analysis
revealed
significant
induction
various
phytohormones
e.g.
abscisic
acid,
salicylic
methyl
jasmonate,
brassinosteroids,
ethrel,
as
well
stresses
like
heat,
cold,
salt,
drought.
To
further
elucidate
's
functional
role,
we
overexpressed
Nicotiana
tabacum
resulting
heightened
resistance
against
Alternaria
solani
pathogen.
Additionally,
observed
enhancements
shoot
length,
root
biomass
transgenic
tobacco
plants
compared
wild
plants.
Notably,
WsOsm‐
overexpressing
seedlings
demonstrated
improved
salt
drought
stress
tolerance,
particularly
at
seedling
stage.
Confocal
histological
H
2
O
biochemical
studies
antioxidant
enzyme
activities
higher
levels
lines,
indicating
enhanced
defense.
Furthermore,
pull‐down
assay
mass
spectrometry
potential
interaction
between
defensin,
known
antifungal
PR
protein
(WsDF).
This
suggests
novel
mediating
responses
by
interacting
with
other
proteins.
Overall,
these
findings
pave
way
for
future
applications
developing
stress‐tolerant
crops
improving
strategies
pathogens.
