International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 94 - 94
Опубликована: Дек. 26, 2024
Green
chemistry
focuses
on
reducing
the
environmental
impacts
of
chemicals
through
sustainable
practices.
Traditional
methods
for
extracting
bioactive
compounds
from
Eucalyptus
marginata
leaves,
such
as
hydro-distillation
and
organic
solvent
extraction,
have
limitations,
including
long
extraction
times,
high
energy
consumption,
potential
toxic
residues.
This
study
explored
use
supercritical
fluid
(SFE),
pressurized
liquid
(PLE),
gas-expanded
(GXL)
processes
to
improve
efficiency
selectivity.
These
techniques
were
combined
in
a
single
mixture
design,
where
CO2
was
used
experiments
carried
out
under
SFE,
while
water
ethanol
PLE
GXL
by
varying
concentration
solvents
cover
all
possibilities.
The
neuroprotective
activity
extracts
evaluated
measuring
their
antioxidant,
anti-inflammatory,
acetylcholinesterase
inhibition
properties.
optimization
resulted
novel
with
an
optimal
ternary
27%
CO2,
55%
ethanol,
18%
water,
degree
desirability
(R2
=
88.59%).
Chromatographic
analysis
GC-MS
HPLC-ESI-MS/MS
identified
over
49
metabolites.
designed
process
offers
promising
approach
producing
phenolic-rich
plant
industrial
applications.
Molecules,
Год журнала:
2025,
Номер
30(1), С. 189 - 189
Опубликована: Янв. 6, 2025
Propolis
is
a
valuable
natural
resource
for
extracting
various
beneficial
compounds.
This
study
explores
sustainable
extraction
approach
Brazilian
green
propolis.
First,
supercritical
fluid
(SFE)
process
parameters
were
optimized
(co-solvent:
21.11%
v/v
CPME,
and
temperature:
60
°C)
to
maximize
yield,
total
phenolic
content
(TPC),
antioxidant
capacity,
LOX
(lipoxygenase)
inhibitory
activity.
GC–MS
analysis
identified
40
metabolites
in
SFE
extracts,
including
fatty
acids,
terpenoids,
phenolics,
sterols.
After
selecting
the
optimum
parameters,
sequential
high-pressure
(HPE)
was
developed,
comprising
SFE,
pressurized
liquid
(PLE)
with
EtOH/H2O,
subcritical
water
(SWE).
compared
similar
using
low-pressure
extractions
(LPE)
Soxhlet
extractor.
The
HPE
achieved
significantly
higher
overall
yield
(80.86%)
than
LPE
(71.43%).
showed
selectivity,
resulting
lower
carbohydrate
non-polar
fraction,
PLE
extracted
nearly
twice
protein
amount
of
LPE–2.
Despite
extracts
exhibited
better
acetylcholinesterase
(AChE),
butyrylcholinesterase
(BChE),
inhibition,
demonstrating
that
neuroprotective
anti-inflammatory
activity
may
be
associated
symbiosis
set
Finally,
comprehensive
greenness
assessment
revealed
proved
more
aligned
chemistry
principles
method.
Plants,
Год журнала:
2025,
Номер
14(2), С. 216 - 216
Опубликована: Янв. 14, 2025
In
this
work,
extracts
from
the
pulp,
peel,
and
seed
of
Manilkara
zapota
were
obtained
via
lyophilization
oven
drying.
Bromatological
analyses
performed
to
investigate
variabilities
in
nutritional
content
fruits
after
nine
post-harvest
days.
The
phytochemical
was
assessed
by
gas
chromatography
flame
ionization
detector
(GC-FID),
their
biological
performance
studied
using
vitro
antibacterial
antioxidant
assays
(DPPH
ABTS)
vivo
toxicity
models.
Molecular
docking
implemented
evaluate
interaction
between
polar
compounds
chicozapote
with
receptors
involved
pathogenesis
bacterial
strains.
Results
revealed
that
water
or
soluble
solids
did
not
vary
post-harvest.
It
demonstrated
oven-drying
approaches
influenced
insoluble,
total
dietary
fiber
digestible
carbohydrates
among
samples.
According
GC-FID
analysis,
it
observed
methods
also
altered
myristic
pentadecanoic
acids
extracts.
noted
activities
weak
due
MIC
(>1000
μg/mL)
IC50
(>2000
values.
Still,
poor
against
Artemia
salina
nauplii.
silico
evaluation
unveiled
M.
possess
a
high
binding
affinity
towards
DNA
gyrase
B
cultured
This
study
expands
scientific
evidence
regarding
influence
distinct
extraction
on
nutraceutical
native
importance
considering
additional
enhance
bioactivities.
Foods,
Год журнала:
2025,
Номер
14(2), С. 297 - 297
Опубликована: Янв. 17, 2025
Sustainable
agro-waste
revaluation
is
critical
to
enhance
the
profitability
and
environmental
footprint
of
olive
oil
industry.
Herein,
valorization
leaf
pruning
waste
from
five
cultivars
('Caiazzana',
'Carolea',
'Itrana',
'Leccino',
'Frantoio')
employed
green
extraction
methods
recover
compounds
with
potential
health
benefits.
Sequential
ultrasound-assisted
maceration
(UAM)
in
n-hexane
ethanol
was
compared
a
compressed
fluid
strategy
consisting
supercritical
(SFE)
pressurized
liquid
(PLE)
for
their
efficiency
recovering
distinct
classes
bioactives.
Chemical
profiling
by
UHPLC-HR-MS/MS
(ultra-high-performance
chromatography
high-resolution
tandem
mass
spectrometry)
GC-MS
(gas
showed
that
UAM-EtOH
effectively
extracted
polyphenols
(especially
luteolin
derivatives)
triterpenes
(notably
maslinic
acid),
while
PLE
yielded
highest
amount
secoiridoids
(e.g.,
secologanoside).
extracts
better
antiradical
activities,
putatively
due
higher
content
flavonoids,
secoiridoids,
HCA
derivatives
than
ones,
as
these
latter
also
contained
20-40%
(cultivar-dependent)
triterpenes.
SFE
concentration
fatty
acids
moderate
antioxidant
activities
but
very
high
AChE
inhibition.
This
study
highlights
importance
selecting
appropriate
methodologies
based
on
target
bioactive
underscores
sustainable
bio-products.
Processes,
Год журнала:
2024,
Номер
12(9), С. 1865 - 1865
Опубликована: Авг. 31, 2024
Nowadays,
supercritical
CO2
extraction
is
highly
regarded
in
industry,
and
several
studies
dealing
with
scale-up
calculations
aim
to
facilitate
the
transition
from
small
scale
large
scale.
To
complete
this
transition,
it
would
be
interesting
able
predict
kinetics,
which
of
work.
A
new
methodology
based
on
association
Sovová’s
broken
intact
cell
model
response
surface
was
developed
SC-CO2
kinetics
different
biomass
(Argan
kernels,
evening
primrose,
Punica
granatum,
Camellia
sinensis,
dry
paprika)
at
operating
conditions
(200–700
bar,
40–60
°C,
0.14–10
kg/h)
inside
an
domain.
The
absolute
average
relative
deviations
between
experimental
predicted
data
ranged
1.86
29.03%,
showing
satisfactory
reliability
methodology.
Fermentation,
Год журнала:
2024,
Номер
10(11), С. 579 - 579
Опубликована: Ноя. 12, 2024
Ginkgo
biloba
extract
(EGB)
has
been
approved
by
the
Food
and
Drug
Administration
in
United
States
for
clinical
studies
on
memory
disorders.
Ginkgolide
B
(GB)
is
major
terpene
lactone
component
of
EGB
a
specific
potent
antagonist
platelet-activating
factor
(PAF).
In
previous
study,
we
reported
medium
composition
conversion
ginkgolides
to
GB
Coprinus
comatus.
present
applied
response
surface
methodology
(RSM)
optimize
conditions
20
L
fermenter
HPLC-MS/MS,
circular
dichroism
(CD)
infrared
(IR)
spectroscopy
analyses,
nuclear
magnetic
resonance
(NMR)
further
confirm
sample
structure.
The
optimal
consisted
12.7
L/min
ventilation,
156
h
time,
132
rpm
rotating
speed,
0.04
MPa
pressure,
27.8
°C
temperature.
Under
conditions,
rate
was
98.62%,
content
higher
than
98%.
CD,
IR,
NMR
analyses
showed
that
molecular
formula
C20H24O10
chemical
structure
good
agreement
with
standard
GB.
Our
current
study
lays
groundwork
industrial
production
