Journal of Pharmaceutical Analysis,
Год журнала:
2024,
Номер
15(3), С. 101152 - 101152
Опубликована: Ноя. 20, 2024
Bacterial
infection
is
a
major
threat
to
global
public
health,
and
can
cause
serious
diseases
such
as
bacterial
skin
foodborne
diseases.
It
essential
develop
new
method
rapidly
diagnose
clinical
multiple
infections
monitor
food
microbial
contamination
in
production
sites
real-time.
In
this
work,
we
developed
4-mercaptophenylboronic
acid
gold
nanoparticles
(4-MPBA-AuNPs)-functionalized
hydrogel
microneedle
(MPBA-H-MN)
for
bacteria
detection
interstitial
fluid.
MPBA-H-MN
could
conveniently
capture
enrich
variety
of
within
5
min.
Surface
enhanced
Raman
spectroscopy
(SERS)
was
then
performed
combined
with
machine
learning
technology
distinguish
identify
bacteria.
Overall,
the
efficiency
exceeded
50%.
concentration
range
1
×
107
1010
colony-forming
units/mL
(CFU/mL),
corresponding
SERS
intensity
showed
certain
linear
relationship
concentration.
Using
random
forest
(RF)-based
learning,
were
effectively
distinguished
an
accuracy
97.87%.
addition,
harmless
disposal
used
MNs
by
photothermal
ablation
convenient,
environmentally
friendly,
inexpensive.
This
technique
provided
potential
rapid
real-time
diagnosis
monitoring
sites.
Polymers,
Год журнала:
2025,
Номер
17(1), С. 104 - 104
Опубликована: Янв. 2, 2025
Recent
studies
have
identified
microneedle
(MN)
arrays
as
promising
alternatives
for
transdermal
drug
delivery.
This
study
investigated
the
properties
of
novel
staggered
MN
design
featuring
two
distinct
heights
MNs.
The
were
precisely
fabricated
via
PμSL
light-cured
3D
printing
technology.
systematically
evaluated
their
morphology,
fracture
force,
skin
penetration
ability,
mechanism,
and
delivery
capability.
results
demonstrated
that
punctured
incrementally,
leveraging
benefits
deformation
during
puncture
process.
approach
effectively
reduced
force
needed,
achieving
a
maximum
reduction
approximately
80.27%
due
to
variations
in
height.
Additionally,
facilitated
penetration,
confirmed
by
rat
hematoxylin-eosin
(H&E)
staining
experiments.
Compared
with
3D-printed
planar
structures
highly
uniform
arrays,
exhibited
enhanced
hydrophilicity,
evidenced
contact
angle
from
93°
70°.
Simulated
release
images
both
coated
hollow
MNs
illustrated
capabilities
these
across
various
layers,
expanded
effective
area
within
vertical
dimension
layers.
offers
experimental
theoretical
foundations
developing
three–dimensional
structural
distributions,
thereby
facilitating
advancements
array
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 2, 2025
Abstract
The
microneedle,
a
minimally
invasive
transdermal
system,
provides
convenient
and
painless
method
for
drug
delivery.
Among
the
various
types
of
microneedles,
hydrogel‐forming
microneedles
(HFMs)
demonstrate
distinct
advantages
in
terms
high‐dose
loading
biocompatibility.
However,
HFMs
usually
require
drying
to
obtain
sufficient
puncture
strength,
which
may
destroy
activity
increase
storage
costs.
Herein,
high‐strength
HFM
patch
with
pH‐responsiveness
post‐drug
long‐term
release
is
developed
based
on
acrylonitrile‐acrylic
acid
copolymer.
dipole‐dipole
hydrogen
bonding
interactions
formed
through
gradient
solvent
replacement
are
evenly
distributed
within
cross‐linked
network,
significantly
enhancing
mechanical
properties
hydrogel
required
epidermal
penetration.
prepared
exhibits
tensile
strength
26
MPa
Young's
modulus
407
MPa.
from
this
display
single
needle
force
1.18
N.
post‐loading
mode
conferred
by
pH
responsiveness
allows
be
encapsulated
both
tips
substrate,
acting
as
reservoir.
Once
applied
skin,
microneedle
activated
body
fluids
achieve
release.
Overall,
improves
hydrated
state,
making
it
promising
delivery
platform.
Biosensors,
Год журнала:
2024,
Номер
14(4), С. 202 - 202
Опубликована: Апрель 19, 2024
Tyrosinase
(TYR)
emerges
as
a
key
enzyme
that
exerts
regulatory
influence
on
the
synthesis
of
melanin,
thereby
assuming
role
critical
biomarker
for
detection
melanoma.
Detecting
authentic
concentration
TYR
in
skin
remains
primary
challenge.
Distinguished
from
ex
vivo
methods,
this
study
introduces
novel
sensor
platform
integrates
microneedle
(MN)
biosensor
with
surface-enhanced
Raman
spectroscopy
(SERS)
technology
situ
human
skin.
The
utilized
dopamine
(DA)-functionalized
gold
nanoparticles
(Au
NPs)
capturing
substrate
and
4-mercaptophenylboronic
acid
(4-MPBA)-modified
silver
(Ag
acting
SERS
probe.
Here,
Au
NPs
were
functionalized
mercaptosuccinic
(MSA)
DA
capture.
In
presence
TYR,
immobilized
MN
is
preferentially
oxidized
to
quinone
(DQ),
process
results
decreased
density
probes
platform.
was
detected
through
variations
signal
intensity
emitted
by
phenylboronic
acid.
system
able
evaluate
concentrations
within
linear
range
0.05
U/mL
200
showed
robust
anti-interference
capabilities.
proposed
platform,
integrating
MN-based
sensing,
technology,
responsiveness,
holds
significant
importance
diagnosing
cutaneous
