Rapid enrichment and SERS differentiation of various bacteria in skin interstitial fluid by 4-MPBA-AuNPs-functionalized hydrogel microneedles DOI Creative Commons
Ying Yang,

Xingyu Wang,

Yexin Hu

и другие.

Journal of Pharmaceutical Analysis, Год журнала: 2024, Номер 15(3), С. 101152 - 101152

Опубликована: Ноя. 20, 2024

Bacterial infection is a major threat to global public health, and can cause serious diseases such as bacterial skin foodborne diseases. It essential develop new method rapidly diagnose clinical multiple infections monitor food microbial contamination in production sites real-time. In this work, we developed 4-mercaptophenylboronic acid gold nanoparticles (4-MPBA-AuNPs)-functionalized hydrogel microneedle (MPBA-H-MN) for bacteria detection interstitial fluid. MPBA-H-MN could conveniently capture enrich variety of within 5 min. Surface enhanced Raman spectroscopy (SERS) was then performed combined with machine learning technology distinguish identify bacteria. Overall, the efficiency exceeded 50%. concentration range 1 × 107 1010 colony-forming units/mL (CFU/mL), corresponding SERS intensity showed certain linear relationship concentration. Using random forest (RF)-based learning, were effectively distinguished an accuracy 97.87%. addition, harmless disposal used MNs by photothermal ablation convenient, environmentally friendly, inexpensive. This technique provided potential rapid real-time diagnosis monitoring sites.

Язык: Английский

Rapid enrichment and SERS differentiation of various bacteria in skin interstitial fluid by 4-MPBA-AuNPs-functionalized hydrogel microneedles DOI Creative Commons
Ying Yang,

Xingyu Wang,

Yexin Hu

и другие.

Journal of Pharmaceutical Analysis, Год журнала: 2024, Номер 15(3), С. 101152 - 101152

Опубликована: Ноя. 20, 2024

Bacterial infection is a major threat to global public health, and can cause serious diseases such as bacterial skin foodborne diseases. It essential develop new method rapidly diagnose clinical multiple infections monitor food microbial contamination in production sites real-time. In this work, we developed 4-mercaptophenylboronic acid gold nanoparticles (4-MPBA-AuNPs)-functionalized hydrogel microneedle (MPBA-H-MN) for bacteria detection interstitial fluid. MPBA-H-MN could conveniently capture enrich variety of within 5 min. Surface enhanced Raman spectroscopy (SERS) was then performed combined with machine learning technology distinguish identify bacteria. Overall, the efficiency exceeded 50%. concentration range 1 × 107 1010 colony-forming units/mL (CFU/mL), corresponding SERS intensity showed certain linear relationship concentration. Using random forest (RF)-based learning, were effectively distinguished an accuracy 97.87%. addition, harmless disposal used MNs by photothermal ablation convenient, environmentally friendly, inexpensive. This technique provided potential rapid real-time diagnosis monitoring sites.

Язык: Английский

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