Harnessing genetic engineering to drive economic bioproduct production in algae

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Янв. 29, 2024

Our reliance on agriculture for sustenance, healthcare, and resources has been essential since the dawn of civilization. However, traditional agricultural practices are no longer adequate to meet demands a burgeoning population amidst climate-driven challenges. Microalgae emerge as beacon hope, offering sustainable renewable source food, animal feed, energy. Their rapid growth rates, adaptability non-arable land non-potable water, diverse bioproduct range, encompassing biofuels nutraceuticals, position them cornerstone future resource management. Furthermore, microalgae’s ability capture carbon aligns with environmental conservation goals. While microalgae offers significant benefits, obstacles in cost-effective biomass production persist, which curtails broader application. This review examines compared other host platforms, highlighting current innovative approaches aimed at overcoming existing barriers. These include range techniques, from gene editing, synthetic promoters, mutagenesis selective breeding metabolic engineering through transcription factors.

Язык: Английский

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Plant secondary metabolites against biotic stresses for sustainable crop protection

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108520 - 108520

Опубликована: Янв. 1, 2025

Язык: Английский

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Transcription factor-based biosensor: A molecular-guided approach for advanced biofuel synthesis

Biotechnology Advances, Год журнала: 2024, Номер 72, С. 108339 - 108339

Опубликована: Март 18, 2024

Язык: Английский

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TFTF: An R-Based Integrative Tool for Decoding Human Transcription Factor–Target Interactions

Biomolecules, Год журнала: 2024, Номер 14(7), С. 749 - 749

Опубликована: Июнь 24, 2024

Transcription factors (TFs) are crucial in modulating gene expression and sculpting cellular organismal phenotypes. The identification of TF-target interactions is pivotal for comprehending molecular pathways disease etiologies but has been hindered by the demanding nature traditional experimental approaches. This paper introduces a novel web application package utilizing R program, which predicts relationships vice versa. Our integrates predictive power various bioinformatic tools, leveraging their combined strengths to provide robust predictions. It merges databases enhanced precision, incorporates correlation accuracy, employs pan-tissue analysis context-specific insights. also enables integration user data with established resources analyze networks. Despite its current limitation human data, it provides platform explore regulatory mechanisms comprehensively. integrated, systematic approach offers researchers an invaluable tool dissecting complexities regulation, potential future expansions include broader range species.

Язык: Английский

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Strategies to increase the robustness of microbial cell factories

Advanced Biotechnology, Год журнала: 2024, Номер 2(1)

Опубликована: Март 1, 2024

Engineering microbial cell factories have achieved much progress in producing fuels, natural products and bulk chemicals. However, industrial fermentation, cells often face various predictable stochastic disturbances resulting from intermediate metabolites or end product toxicity, metabolic burden harsh environment. These perturbances can potentially decrease productivity titer. Therefore, strain robustness is essential to ensure reliable sustainable production efficiency. In this review, the current strategies improve host were summarized, including knowledge-based engineering approaches, such as transcription factors, membrane/transporters stress proteins, traditional adaptive laboratory evolution based on selection. Computation-assisted (e.g. GEMs, deep learning machine learning) design of robust hosts was also introduced. Furthermore, challenges future perspectives are proposed promote development green, efficient biomanufacturers.

Язык: Английский

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GATA2 promotes M1 macrophage polarization and inhibits the malignant progression of lung adenocarcinoma through transcription activation-mediated upregulation of KANK3

Molecular & Cellular Toxicology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

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Tạo dòng, biểu hiện và tinh sạch OsbHLH061, một nhân tố điều hòa phiên mã cảm ứng khô hạn ở lúa, trên vi khuẩn E. coli

Can Tho University Journal of Science, Год журнала: 2025, Номер 61(1)

Опубликована: Фев. 14, 2025

OsbHLH061 là một nhân tố điều hòa phiên mã thuộc họ basic helix-loop-helix (bHLH) và được dự đoán hoạt động trong quá trình chống chịu với hạn ở lúa. Nhằm làm rõ vai trò cơ chế của OsbHLH061, việc xác định các tự DNA liên kết đặc hiệu protein này rất cần thiết, vì từ đó có thể gen mục tiêu OsbHLH061. Do đó, nghiên cứu thực hiện nhằm tạo dòng, biểu E. coli tinh sạch cho phản ứng protein-DNA in vitro. Vector lựa chọn pET-32a sự mặt thẻ tăng tính tan (Thioredoxin) khả năng (Histidine). Kết quả đã dòng thành công pET-HLH061 coli, thiết kế để dung hợp Thioredoxin Histidine đầu N. Protein tốt pha bằng phương pháp sắc kí ái lực Nikel chất lượng đủ đề ra.

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Green Tea Polyphenol Epigallocatechin-3-gallate Mediates an Antioxidant Response via Nrf2 Pathway in Heat-Stressed Poultry: A Review

Poultry Science, Год журнала: 2025, Номер 104(5), С. 105071 - 105071

Опубликована: Март 20, 2025

Heat stress is a critical challenge in the poultry industry. It arises when birds are exposed to elevated ambient temperatures beyond their thermoneutral zone, often exacerbated by high humidity and inadequate ventilation. This condition disrupts birds' ability maintain thermal homeostasis, leading physiological behavioral changes such as increased panting, reduced feed intake, water consumption. These responses aim dissipate heat but result energy imbalances, oxidative stress, impaired immune function. Green tea polyphenols (GTPs) mitigate modulating pathways, primarily scavenging reactive oxygen species (ROS) enhancing antioxidant defense mechanisms. pathways play pivotal role neutralizing ROS generated during inflammation, exposure electrophilic compounds. action helps restore cellular balance enhances overall mechanisms converting harmful free radicals into less molecules, oxygen. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) plays significant character activation of enzymatic antioxidants network. translocates nucleus upon activation, binds response elements (AREs) promoter regions target genes, upregulates expression key enzymes. Therefore, regulation Nrf2 considered molecular marker mitigating effects its detoxification enzymes, protecting against damage inflammation induced temperatures. exploratory review summarizes anti-oxidative GTPs poultry. highlights cytoprotective basis epigallocatechin-3-gallate (EGCG), particularly Nrf2-mediated which enhance systems protect damage.

Язык: Английский

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RNA-Seq of Chicken Embryo Liver Reveals Transcriptional Pathways Influenced by Egg Formaldehyde Treatment

Genes, Год журнала: 2025, Номер 16(5), С. 471 - 471

Опубликована: Апрель 22, 2025

Background/Objectives: Hatchery fumigation is recognized as a crucial step to control microbial bloom in the environment, and formaldehyde one of most widely used disinfectants ensure successful hatchability healthy production. While many benefits are thought be derived from disinfectant properties, it possible that additional host gene genetic pathway modulation could contribute these outcomes. The current study aimed capture ovo transcriptional response liver tissue treatment. Methods: Chick embryos were subjected treatment for 25 min at 24–25 °C 75% relative humidity, keeping group untreated. On 18th day incubation 37.8 58–63% eggs broken, was obtained RNA isolation, cDNA library preparation, sequencing. Results: Bioinformatics analysis revealed 908 significant differentially expressed genes (DEGs), among which 814 known 94 novel genes. A total 672 DEGs upregulated, whereas 236 downregulated FA Of genes, 80 upregulated. Key DEGs, associated QTLs, transcription factors involved immuno-inflammatory responses, oxidative stress, epigenetic modification, cellular adaptation-related activities. Further research should focus on biological validation key clarify their roles, pathways, relationships Conclusions: Overall, findings (1) provide critical molecular detail first towards selection improve effectiveness, (2) DEG signatures reference against compare other interventions achieve production benefits.

Язык: Английский

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Genome-Wide Investigation of MADS-Box Genes in Flower Development and Environmental Acclimation of Lumnitzera littorea (Jack) Voigt

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1680 - 1680

Опубликована: Фев. 16, 2025

Lumnitzera littorea (Jack) Voigt is an endangered mangrove species in China. Low fecundity and environmental pressure are supposed to be key factors limiting the population expansion of L. littorea. Transcription with MADS-box domain crucial regulators plant flower development, reproduction, stress response. In this study, we performed a comprehensive investigation into features functions genes Sixty-three LlMADS similar structure motif composition were identified genome, these unevenly distributed on 11 chromosomes. Segmental duplication was suggested make main contribution gene family. Some LIMADS exhibited differential expression different types or response cold stress. Overexpression B-class LlMADS37 had substantial effects morphology flowering time transgenic Arabidopsis plants, demonstrating its role regulating morphogenesis inflorescence. These findings largely enrich our understanding functional importance inflorescence acclimation provide valuable resources for future genetic research improve conservation species.

Язык: Английский

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