Inflammatory Bowel Disease: A Comprehensive Analysis of Molecular Bases, Predictive Biomarkers, Diagnostic Methods, and Therapeutic Options
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(13), С. 7062 - 7062
Опубликована: Июнь 27, 2024
In
inflammatory
bowel
diseases
(IBDs),
such
as
Crohn's
disease
(CD)
and
ulcerative
colitis
(UC),
the
immune
system
relentlessly
attacks
intestinal
cells,
causing
recurrent
tissue
damage
over
lifetime
of
patients.
The
etiology
IBD
is
complex
multifactorial,
involving
environmental,
microbiota,
genetic,
immunological
factors
that
alter
molecular
basis
organism.
Among
these,
microbiota
cells
play
pivotal
roles;
generates
antigens
recognized
by
antibodies,
while
autoantibodies
target
attack
membrane,
exacerbating
inflammation
damage.
Given
altered
framework,
analysis
multiple
biomarkers
in
patients
proves
exceedingly
valuable
for
diagnosing
prognosing
IBD,
including
markers
like
C
reactive
protein
fecal
calprotectin.
Upon
detection
classification
patients,
specific
treatments
are
administered,
ranging
from
conventional
drugs
to
new
biological
therapies,
antibodies
neutralize
molecules
tumor
necrosis
factor
(TNF)
integrin.
This
review
delves
into
targets,
biomarkers,
treatment
options,
monitoring
techniques,
and,
ultimately,
current
challenges
management.
Язык: Английский
Emerging Tools for Generating Genomics Data
Опубликована: Янв. 1, 2024
Язык: Английский
Transplant of gut microbiota ameliorates metabolic and heart disorders in rats fed with a hypercaloric diet by modulating microbial metabolism and diversity
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
181, С. 117667 - 117667
Опубликована: Ноя. 15, 2024
Язык: Английский
Proteomic and Metabolomic Profiling of Archaeal Extracellular Vesicles from the Human Gut
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 22, 2024
Abstract
One
potential
mechanism
for
microbiome-host,
and
microbiome
constituents’
interaction
communication
involves
extracellular
vesicles
(EVs).
Here,
the
first
time,
we
report
capability
of
two
M.
smithii
strains
(ALI
GRAZ-2),
Candidatus
intestini,
Methanosphaera
stadtmanae,
as
underrepresented
components
gut
microbiome,
to
produce
EVs.
Interesting,
size,
morphology,
composition
AEVs
were
comparable
bacterial
EVs,
indicated
by
ultrastructure,
composition,
proteomic
metabolomic
analyses;
however,
EVs
substantially
less
prevalent
in
studied
Archaea.
When
looking
at
proteomics
more
precisely,
although
from
ALI
intestini
found
be
carrying
unique
proteins
(n=135
n=30,
respectively),
shared
within
this
genus
(n=229),
mostly
adhesins(/like)
proteins,
or
with
IG-like
domains.
remarkable
observation
was
uptake
obtained
stadtmanae
Methanobrevibacter
species
human
monocytes
subsequent
IL-8
secretion.
Язык: Английский
Reconstruction and identification of the native PLP synthase complex from Methanosarcina acetivorans lysate
Опубликована: Июль 10, 2024
Abstract
Many
protein-protein
interactions
behave
differently
in
biochemically
purified
forms
as
compared
to
their
vivo
states.
As
such,
determining
native
protein
structures
may
elucidate
structural
states
previously
unknown
for
even
well-characterized
proteins.
Here
we
apply
the
bottom-up
proteomics
method,
cryoID
,
toward
a
model
methanogenic
archaeon.
While
they
are
keystone
organisms
global
carbon
cycle
and
active
members
of
human
microbiome,
there
is
general
lack
characterization
methanogen
enzyme
structure
function.
Through
approach,
successfully
reconstructed
identified
Methanosarcina
acetivorans
pyridoxal
5’-phosphate
(PLP)
synthase
(PdxS)
complex
directly
from
cryogenic
electron
microscopy
(cryoEM)
images
fractionated
cellular
lysate.
We
found
that
PdxS
exists
homo-dodecamer
subunits,
proposed
supracomplex
containing
both
glutaminase
(PdxT)
was
not
observed
Our
shows
monomer
fashions
single
8α/8β
TIM-barrel
domain,
surrounded
by
seven
additional
helices
mediate
solvent
interface
contacts.
A
density
present
at
site
cryoEM
map
interpreted
ribose
5-phosphate.
In
addition
being
first
reconstruction
heterogeneous
sample,
our
results
reveal
departure
published
archaeal
crystal
structures,
lacking
37
amino
acid
insertion
these
prior
cases.
This
study
demonstrates
potential
applying
workflow
capture
atomic
resolution
systems,
which
traditional
biochemical
sample
preparation
nontrivial.
Язык: Английский
Proteomic and Metabolomic Profiling of Archaeal Extracellular Vesicles from the Human Gut
Опубликована: Июль 17, 2024
Abstract
Microorganisms
thriving
in
the
human
gastrointestinal
microbiome
have
developed
numerous
mechanisms
for
microbiome-host
and
microbe-microbe
interaction,
including
formation
of
extracellular
vesicles
(EVs).
However,
interactive
members
archaeome
remained
largely
unexplored.
Here,
we
report
capability
two
Methanobrevibacter
strains
M.
smithii,
Cand.
intestini
Methanosphaera
stadtmanae,
to
produce
EVs.
While
size
(~
130
nm)
morphology
these
archaeal
EVs
were
comparable
bacterial
EVs,
proteomic
metabolomic
analyses
revealed
unique
traits.
The
proteins
found
both
smithii
(n
=
229)
predominantly
included
adhesin
adhesin-like
proteins,
involved
archaea-surface
archaea-bacteria
interaction.
Additionally,
contained
glutamic
acid
choline
glycerophosphate,
which
could
be
gut-brain
signalling.
Vesicles
from
all
studied
species
efficiently
taken
up
by
monocytes
induced
IL-8
production
epithelial
HT-29
cells.
identification
their
components
provides
novel
insights
into
complex
interactions
between
host,
potentially
offering
new
paths
modulate
vesicle-mediated
impacts
on
host
health.
Язык: Английский
Structure and identification of the native PLP synthase complex from Methanosarcina acetivorans lysate
mBio,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 26, 2024
Many
protein-protein
interactions
behave
differently
in
biochemically
purified
forms
as
compared
to
their
Язык: Английский