L-theanine promotes angiogenesis in limb ischemic mice by modulating NRP1/VEGFR2 signaling

Biomolecules and Biomedicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 7, 2025

Peripheral artery disease (PAD), primarily caused by atherosclerosis, leads to the narrowing or blockage of arteries that supply blood limbs. This study explores pro-angiogenic effects L-theanine and its underlying mechanisms in a mouse model hindlimb ischemia (HLI). To evaluate L-theanine’s effects, human umbilical vein endothelial cells (HUVECs) were subjected tube formation, migration, sprouting, proliferation assays. In vivo, C57BL/6 mice with induced HLI treated L-theanine. Blood flow recovery was measured via Doppler ultrasound, vascular density analyzed using immunofluorescence staining. RNA sequencing identified neuropilin-1 (NRP1) as key regulator, expression levels NRP1 VEGFR2 examined through qPCR Western blotting. significantly enhanced angiogenesis HUVECs, demonstrated improved proliferation. mice, treatment resulted increased vessel recovery. Furthermore, found activate NRP1/VEGFR2 signaling pathway both HUVECs model. These findings indicate can promote pathways involved repair, suggesting potential therapeutic agent for treating defects associated PAD.

Язык: Английский

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l-Glutamic Acid Alleviates Mastitis in Dairy Cows by Targeting the Sirt5/Nrf2/Prdx1 Signaling Axis and Mitochondrial Function

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

Mastitis in dairy cows is an inflammatory disease that severely affects the health and lactation functions of cows. Mitochondrial damage closely related to response. How effectively alleviate mitochondrial key preventing treating mastitis In this study, we found elevated levels response accompanied by reduced expression Sirt5 (Sirtuin5) with compared healthy This suggests plays important role Subsequently, further analyzed mammary gland tissue from untargeted metabolomics (LC-MS/MS) screened for differential metabolite l-glutamic acid (l-Glu). To validate effect l-Glu on cows, conducted a study using MAC-T cells. The results showed was able ameliorate LPS-induced activating promoting fusion upregulation membrane potential (MMP) levels. contrast, unable protect function after knocking down Sirt5. Furthermore, upregulate nuclear factor E2-related (Nrf2) peroxiredoxin 1 (Prdx1) cells, promoted entry Nrf2 into nucleus, which reversed Next, explored whether alleviates through Nrf2/Prdx1 signaling axis inhibitor RA. use RA blocked protective function. conclusion, ameliorates targeting activate provides new target theoretical basis clinical control mastitis. could be added as dietary supplement diets maintain homeostasis, thereby protecting economic value

Язык: Английский

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L-Theanine attenuates oxidative damage induced by heat stress through the PI3K/AKT/Nrf2 signaling pathway in skeletal muscle cells

Poultry Science, Год журнала: 2025, Номер unknown, С. 105140 - 105140

Опубликована: Апрель 1, 2025

The rising prevalence of heat stress (HS), because global warming, presents a considerable challenge to both human and animal health welfare. L-Theanine (LTA), naturally occurring amino acid, may enhance poultry muscle yield quality, suggesting its potential application in alleviating the negative impacts HS. However, molecular mechanisms through which LTA exerts beneficial effects remain be fully understood. This study explored protective on cultured broiler skeletal cells under oxidative induced by HS, focusing involved. Our findings indicate that treatment with significantly improved cell survival, bolstered activity antioxidant enzymes, decreased reactive oxygen species (ROS) contents diminished malondialdehyde (MDA) levels HS-treated cells. Furthermore, enhanced phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) nuclear factor erythroid 2-related 2 (Nrf2) signaling pathways cells, facilitating mitochondrial biogenesis reducing apoptosis. were reduced PI3K inhibitor LY294002 or Nrf2 ML385. In conclusion, our showed protects from HS-induced damage modulating PI3K/AKT/Nrf2 pathway, positioning as promising natural for feed additives aimed at improving health.

Язык: Английский

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Cardioprotective effect of senotherapy in chronically obese middle-aged female rats may be mediated by a MERCSs/Nrf2 interaction.

The Journal of Nutritional Biochemistry, Год журнала: 2025, Номер unknown, С. 109923 - 109923

Опубликована: Апрель 1, 2025

Язык: Английский

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L-theanine prevents myocardial injury in sleep‑deprived mice by suppressing ferroptosis through SIRT1

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Май 6, 2025

Язык: Английский

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