International Journal of Biological Macromolecules, Год журнала: 2024, Номер unknown, С. 138996 - 138996
Опубликована: Дек. 1, 2024
Язык: Английский
Analytica Chimica Acta, Год журнала: 2024, Номер 1316, С. 342843 - 342843
Опубликована: Июнь 7, 2024
Язык: Английский
Процитировано
4
TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118172 - 118172
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 303, С. 140675 - 140675
Опубликована: Фев. 4, 2025
Язык: Английский
Процитировано
0
ACS Sensors, Год журнала: 2025, Номер unknown
Опубликована: Фев. 14, 2025
Strand displacement amplification (SDA) is an isothermal DNA technique. Herein, we developed a novel SDA system, designated All-U-Want (AUW-SDA), which was used as signal strategy for the construction of nucleic acid detection biosensors. AUW-SDA capable target turnover and can be utilized sequences without available 3′-ends. Of particular significance ability to generate substantial number programmable in accordance with specifications sensor output methods, irrespective sequence acid. We N gene SARS-CoV-2 model develop sensing platform dual outputs. The colorimetric signals were generated by G-quadruplex/hemin DNAzyme, G-rich produced C-rich primer. On other hand, altering within replaceable region primer, activator obtained from AUW-SDA, could trigger activity CRISPR/Cas12a, cleaving probes modified fluorophore quencher at each end subsequently yielding fluorescent signals. After reaction conditions optimized, limit (LOD) values assays estimated 0.672 fM 13.3 fM, respectively. biosensors biological sample detection. reliability proposed method validated against RT-qPCR results. In addition, portable scanner-assisted high-throughput RGB analysis (PSHRA) developed. This applied our biosensor multilocus SARS-CoV-2. results satisfactory, indicating potential this approach field testing or point-of-care (POC) diagnostics.
Язык: Английский
Процитировано
0
Bioelectrochemistry, Год журнала: 2025, Номер unknown, С. 108975 - 108975
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Опубликована: Апрель 8, 2025
Review CRISPR-Cas System: Novel Experimental Therapeutic and Diagnostic Approaches for Chronic Liver Diseases Teja Naveen Sata Senthil Kumar Venugopal * Faculty of Life Sciences Biotechnology, South Asian University, New Delhi 110068, India Correspondence: [email protected]; Tel.: +91-1135656620; Fax: +91-1124122511 Received: 7 February 2025; Accepted: 26 Published: 8 April 2025 Abstract: liver disease (CLD), a significant ailment, contributes to nearly two million deaths annually. CLD can be caused by alcohol consumption, fat, viral infections, genetic disorders. Accurate diagnosis application therapeutics are crucial strategies enhancing the management CLD. The system, originally prokaryotic innate immunity mechanism, has evolved into current-generation tool therapeutic diagnostic applications. cis-cleavage feature system involves crRNA-guided specific target cleavage. This mechanism is utilized development therapeutics. Few systems possess additional trans-cleavage, which non-specific cleavage, also known as collateral unique exploited generate diagnostics. In hepatitis, CRIPSR-Cas have been concurrently applied reported genome-targeted detection systems. Research on alcoholic non-alcoholic fatty diseases mainly focuses targeting progression factors. Also, CRISPR-Cas-based gene editing used manage hepatocellular carcinoma, oncogene-targeted therapies biomarker Various non-viral delivery proposed developing Despite limited progress, potential broader in review describes comprehensive use experimental approaches
Язык: Английский
Процитировано
0
Journal of Analysis and Testing, Год журнала: 2024, Номер unknown
Опубликована: Дек. 20, 2024
Язык: Английский
Процитировано
2
Biosensors, Год журнала: 2024, Номер 14(9), С. 419 - 419
Опубликована: Авг. 27, 2024
Point-of-care testing (POCT) is a contemporary diagnostic approach characterized by its user-friendly nature, cost efficiency, environmental compatibility, and lack of reliance on professional experts. Therefore, it widely used in clinical diagnosis other analytical fields to meet the demand for rapid convenient testing. The application POCT technology not only improves but also brings convenience benefits healthcare industry. personal glucose meter (PGM) highly successful commercial tool that has been analysis, non-glucose target detection. In this review, recent advances from 2020 2024 analysis PGMs as devices are summarized. signal transduction strategies based PGMs, including enzymatic transduction, nanocarrier (enzyme or glucose), consumption briefly introduced. Meanwhile, applications outlined, encompassing biomedical, environmental, food along with diverse applications. Finally, prospects obstacles employing tools discussed.
Язык: Английский
Процитировано
1
Analysis & Sensing, Год журнала: 2024, Номер 4(4)
Опубликована: Апрель 17, 2024
Abstract In this study, we propose an enhanced nanopore sensing strategy that utilizes a peptide nucleic acid (PNA)‐based triplex molecular beacon sensor to achieve the simultaneous detection of multiple biomarkers with high degree sensitivity. The is switch composed triplex‐forming DNA strand and oligo‐arginine‐tagged PNA strand, serving as target recognition moiety signal output element, respectively. Upon binding element sensor, released hybrid complex target‐DNA formed simultaneously. Due positive charges carried by PNA‐Arg strands, they could be driven through under electric field, effectively eliminating interferences from co‐existing complexes. This approach enables label‐free, one‐step targets without requiring treatments procedures. Leveraging modular properties method can applied universally, here, successfully demonstrate its application using three SARS‐CoV‐2 related biomarkers.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
The CRISPR-Cas13 systems have opened a new avenue for RNA detection due to their exceptional programmable collateral activity against ssRNA. However, most high-performance Cas13-based sensors still suffer from some drawbacks because of the tradeoffs between readout device complexity and analytical sensitivity, which can limit viability at point-of-care (POC). To overcome these shortcomings, we herein report novel target-responsive POC platform translating into glucose test. Specifically, this combines advantages three techniques: hybrid Cas13a/Cas12a system both recognition with single-base resolution cascade enzymatic amplification, self-sustainable catalytic nucleic acid circuit (abbreviated as SCC) embedded uracil-base poly-T bulges Cas13a- Cas12a-mediated cleavage, respectively, portable meter (PGM) simple signal readout. incorporation an engineered ssDNA–invertase conjugate in SCC led RNA-to-glucose transduction through hydrolysis sucrose glucose. By targeting 20-nt conserved region BMAL1 mRNA (mBMAL1), key circadian clock gene, demonstrate that serve excellent CRISPR reporter, enabling direct unamplified mBMAL1 low 0.864 fM concurrently good discrimination ability mismatch. More importantly, integrated was successfully applied diagnosis different cell lysates, results strongly correlate RT-PCR. Given its wide adaptability, anticipate such CRISPR-SCC be easily modified quantify other biomarkers associated rhythm.
Язык: Английский
Процитировано
0