International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 10090 - 10090
Опубликована: Сен. 19, 2024
The
emergence
of
antibiotic-resistant
Streptococcus
pneumoniae
necessitates
the
discovery
novel
therapeutic
agents.
This
study
investigated
antimicrobial
potential
green-synthesized
gold
nanoparticles
(AuNPs)
fabricated
using
Arthrospira
platensis
extract.
Characterization
Fourier
transform
infrared
spectroscopy
revealed
presence
functional
groups
such
as
ketones,
aldehydes,
and
carboxylic
acids
in
capping
agents,
suggesting
their
role
AuNP
stabilization.
Transmission
electron
microscopy
demonstrated
formation
rod-shaped
AuNPs
with
a
mean
diameter
134.8
nm,
determined
by
dynamic
light
scattering,
zeta
−27.2
mV,
indicating
good
colloidal
stability.
synthesized
exhibited
potent
antibacterial
activity
against
S.
pneumoniae,
minimum
inhibitory
concentration
(MIC)
12
μg/mL,
surpassing
efficacy
control
antibiotic,
tigecycline.
To
elucidate
underlying
mechanisms
action,
an
untargeted
metabolomic
analysis
A.
extract
was
performed,
identifying
26
bioactive
compounds
belonging
to
diverse
chemical
classes.
In
silico
studies
focused
on
molecular
docking
simulations
that
compound
22
strong
binding
affinity
topoisomerase
IV,
critical
enzyme
for
bacterial
DNA
replication.
Molecular
dynamics
further
validated
stability
this
protein–ligand
complex.
These
findings
collectively
highlight
promising
platensis-derived
constituent
compounds,
warranting
investigation
development
anti-pneumococcal
therapeutics.
Background:
Liver
cancer
is
increasing
in
different
parts
of
the
world
and
fourth
leading
cause
death
globally.
Objectives:
The
present
study
aims
to
synthesize
analyze
characterization
gold
nanoparticles
(AuNPs)
synthesized
by
Cichorium
intybus
extract
evaluate
their
antioxidant
cellular
toxicity
activity
against
liver
cells
(HepG2).
Methods:
AuNPs
were
characterized
using
X-ray
diffraction
(XRD),
field
emission
scanning
electron
microscopy
(FESEM),
fourier-transform
infrared
spectroscopy
(FTIR).
was
assessed
DPPH
test,
cytotoxicity
analyzed
MTT
method.
Results:
results
indicate
that
are
crystalline
materials
with
a
particle
size
less
than
100
nm,
mean
23.94
nm.
FTIR
reveals
presence
biochemical
groups
act
as
reducing
factors.
demonstrate
increases
concentration,
87%
inhibition
free
radical
scavenging
observed
at
250
µg/mL.
Cell
cell
lines
(HepG2)
demonstrated
significant
time-
dose-dependent
manner.
percentage
viability
concentration
1000
µg/ml
after
24,
48,
72
hours
determined
be
45%,
51%,
22%,
respectively.
Conclusions:
revealed
simple
cost-effective
environmentally
friendly
method
could
employed
from
food
pharmaceutical
industries.
Chemistry - An Asian Journal,
Год журнала:
2024,
Номер
19(23)
Опубликована: Сен. 2, 2024
Abstract
The
Zika
virus
(ZIKV)
is
a
global
health
threat
due
to
its
rapid
spread
and
severe
implications,
including
congenital
abnormalities
neurological
complications.
Differentiating
ZIKV
from
other
arboviruses
such
as
dengue
(DENV)
crucial
for
effective
diagnosis
treatment.
This
study
presents
the
development
of
biosensor
detecting
non‐structural
protein
1
(NS1)
using
gold
nanoparticles
(AuNPs)
functionalized
with
monoclonal
antibodies
employing
dynamic
light
scattering
(DLS).
named
ZINS1‐mAb‐AuNP
exhibited
specific
binding
NS1
protein,
demonstrating
high
colloidal
stability
indicated
by
hydrodynamic
diameter
(D
H
)
140
nm,
detectable
via
DLS.
In
absence
ionic
strength
medium
caused
particle
aggregation.
detection
method
showed
good
sensitivity
specificity,
limit
(LOD)
0.96
μg
mL
−1
,
avoided
cross‐reactivity
DENV2
SARS‐CoV‐2
spike
proteins.
represents
promising
tool
early
accurate
ZIKV,
facilitating
diagnostic
treatment
capabilities
arboviral
infections.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Сен. 17, 2024
Diabetes
mellitus
(DM)
is
a
prevalent
disorder
with
an
urgent
need
for
continuous,
precise,
and
on-site
biomarker
monitoring
devices.
The
continuous
of
DM
biomarkers
from
different
biological
matrices
will
become
routine
in
the
future,
thanks
to
promising
biosensor
design.
Lately,
employing
nanomaterials
receptor
parts
has
had
great
impact
on
smart
monitoring.
Among
them,
gold
nanostructures
(AuNSs)
have
arisen
as
highly
potential
materials
fabricating
precise
biosensors
due
their
unique
properties.
present
study
provides
update
applications
AuNSs
detecting
glucose
well
other
biomarkers,
such
glycated
hemoglobin
(HbA1c),
albumin
(GA),
insulin,
insulin
antibodies,
uric
acid,
lactate,
glutamic
acid
decarboxylase
antibodies
(GADA),
focus
most
important
factors
performance
sensitivity,
selectivity,
response
time,
stability.
Specified
values
limit
detection
(LOD),
linear
concentrations,
reproducibility%,
recovery%,
assay
time
were
used
compare
studies.
In
conclusion,
AuNSs,
owing
wide
electrochemical
window
low
electrical
resistivity,
are
valuable
tools
design,
alongside
reagents
and/or
nanomaterials.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 10090 - 10090
Опубликована: Сен. 19, 2024
The
emergence
of
antibiotic-resistant
Streptococcus
pneumoniae
necessitates
the
discovery
novel
therapeutic
agents.
This
study
investigated
antimicrobial
potential
green-synthesized
gold
nanoparticles
(AuNPs)
fabricated
using
Arthrospira
platensis
extract.
Characterization
Fourier
transform
infrared
spectroscopy
revealed
presence
functional
groups
such
as
ketones,
aldehydes,
and
carboxylic
acids
in
capping
agents,
suggesting
their
role
AuNP
stabilization.
Transmission
electron
microscopy
demonstrated
formation
rod-shaped
AuNPs
with
a
mean
diameter
134.8
nm,
determined
by
dynamic
light
scattering,
zeta
−27.2
mV,
indicating
good
colloidal
stability.
synthesized
exhibited
potent
antibacterial
activity
against
S.
pneumoniae,
minimum
inhibitory
concentration
(MIC)
12
μg/mL,
surpassing
efficacy
control
antibiotic,
tigecycline.
To
elucidate
underlying
mechanisms
action,
an
untargeted
metabolomic
analysis
A.
extract
was
performed,
identifying
26
bioactive
compounds
belonging
to
diverse
chemical
classes.
In
silico
studies
focused
on
molecular
docking
simulations
that
compound
22
strong
binding
affinity
topoisomerase
IV,
critical
enzyme
for
bacterial
DNA
replication.
Molecular
dynamics
further
validated
stability
this
protein–ligand
complex.
These
findings
collectively
highlight
promising
platensis-derived
constituent
compounds,
warranting
investigation
development
anti-pneumococcal
therapeutics.
