Maternal exposure to dim light at night induces behavioral alterations in the adolescent and adult offspring Wistar rat

Frontiers in Physiology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

Access to electric light has exposed living organisms varying intensities of throughout the 24 h day. Dim at night (DLAN) is an inappropriate signal for biological clock, which responsible circadian organization physiology. During gestational period, physiological adaptations occur ensure a successful pregnancy and optimal fetal development. Environmental maternal conditions, such as disruptions rhythms, could negatively affect offspring health. We have previously demonstrated that exposure female Wistar rats DLAN results in circadian, metabolic, behavioral alterations. A relevant behavior during adolescence social play, primarily regulated by nucleus accumbens (NAc) crucial proper performance important behaviors adulthood. Throughout development, microglia are remodeling diverse brain regions via synaptic pruning. adolescence, this process occurs within NAc, where immune-mediated directly impacts play behavior. This study investigated effects or light-dark cycle (LD) before (5 weeks) period (21-23 days) on metabolism Body mass was measured every 5 days from postnatal day 1 (PN1) PN25 10 PN40 PN90; food consumption monitored weekly PN90. Social evaluated PN40. The quantification morphology NAc were PN30. An open field test conducted PN60, anhedonia assessed Male mothers showed increased body gain PN25. male had lower consumption, while females exhibited consumption. In behavior, no differences found between LD offspring. contrast, significant decrease compared animals, associated with higher numbers more ramified morphology. Importantly, PN90, presented anxiety-like behaviors. These demonstrate induces intergenerational alterations persist until

Язык: Английский

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Leveraging endophytic fungi and multiomics integration for targeted drug discovery

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 277 - 293

Опубликована: Янв. 1, 2025

Язык: Английский

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Circadian clock features define novel subtypes among breast cancer cells and shape drug sensitivity

Molecular Systems Biology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

Abstract The circadian clock regulates key physiological processes, including cellular responses to DNA damage. Circadian-based therapeutic strategies optimize treatment timing enhance drug efficacy and minimize side effects, offering potential for precision cancer treatment. However, applying these in remains limited due a lack of understanding the clock’s function across types incomplete insights into how affects responses. To address this, we conducted deep phenotyping panel breast cell lines. Observing diverse dynamics, characterized metrics assess rhythm strength stability vitro. This led identification four distinct circadian-based phenotypes among 14 models: functional, weak, unstable, dysfunctional clocks. Furthermore, demonstrate that plays critical role shaping pharmacological various anti-cancer drugs identify features descriptive sensitivity. Collectively, our findings establish foundation implementing cancer, leveraging sensitivity patterns outcomes.

Язык: Английский

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Checkpoint kinases regulate the circadian clock after DNA damage by influencing chromatin dynamics

Nucleic Acids Research, Год журнала: 2025, Номер 53(5)

Опубликована: Фев. 27, 2025

The interplay between circadian clocks, the cell cycle, and DNA repair has been extensively documented, yet epigenetic control of clocks by damage responses remains relatively unexplored. Here, we showed that checkpoint kinases CHK1/2 regulate chromatin structure during in Neurospora crassa to maintain robust rhythms. Under stress, deletion chk1/2 disrupted rhythmic transcription clock gene frq suppressing binding activator White Collar complex (WCC) at promoter, as remained condensed. Mechanistically, interacted with WC-2 were recruited WCC bind promoter phosphorylate H3T11, promoting H3 acetylation, especially H3K56 counteract histone variant H2A.Z deposition, thereby establishing a suitable state rhythms despite damage. Additionally, genome-wide correlation was discovered H3T11 phosphorylation showing specific function is dependent on CHK1/2. Furthermore, transcriptome analysis revealed are responsible for metabolic genes These findings highlight essential role maintaining under stress.

Язык: Английский

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Circadian clock is critical for fungal pathogenesis by regulating zinc starvation response and secondary metabolism

Science Advances, Год журнала: 2025, Номер 11(13)

Опубликована: Март 28, 2025

Circadian clocks are known to modulate host immune responses pathogen infections, yet their role in influencing pathogenesis remains unclear. Here, we investigated the of circadian regulating fungal Fusarium oxysporum , which has multiple genes homologous Neurospora crassa frq due gene duplication events, with Fofrq1 being primary clock gene. The F. plants is controlled by its clock, infections causing severe disease symptoms at dawn. Notably, disruption dramatically reduces pathogenicity. regulate rhythmic expression several transcription factors, including FoZafA, enables adapt zinc starvation within plant, and FoCzf1, governs production toxin fusaric acid. Together, our findings highlight critical roles pathogenicity response secondary metabolite production.

Язык: Английский

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