The DEAD-box helicase eIF4A1 acts as RNA chaperone during mitotic exit enabling chromatin decondensation DOI Creative Commons
Wolfram Antonin, Ramona Jühlen,

Sabine C. Wiesmann

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 20, 2024

Abstract During mitosis, chromosomes condense and decondense to segregate faithfully undamaged. The exact molecular mechanisms are not well understood. We identify the DEAD-box helicase eIF4A1 as a critical factor in this process. In cell-free condensation assay is crucial for process, relying on its RNA-binding ability but ATPase activity. Reducing levels cells consistently slows down chromatin decondensation during nuclear reformation. Conversely, increasing concentration mitotic accelerates their decondensation. absence of affects perichromatin layer, which surrounds mitosis consists RNA mainly nucleolar proteins. vitro, acts an chaperone, dissociating biomolecular condensates chaperone activity required regulate composition fluidity dynamic reorganization exit mitosis.

Язык: Английский

Formation, function, and pathology of RNP granules DOI Creative Commons
Nina Ripin,

Roy Parker

Cell, Год журнала: 2023, Номер 186(22), С. 4737 - 4756

Опубликована: Окт. 1, 2023

Язык: Английский

Процитировано

80
RNA granules in neuronal plasticity and disease DOI Open Access

Karl E. Bauer,

Bruna R. de Queiroz, Michael Kiebler

и другие.

Trends in Neurosciences, Год журнала: 2023, Номер 46(7), С. 525 - 538

Опубликована: Май 16, 2023

Язык: Английский

Процитировано

28
The Story of RNA Unfolded: The Molecular Function of DEAD- and DExH-Box ATPases and Their Complex Relationship with Membraneless Organelles DOI
Kerstin Dörner, Maria Hondele

Annual Review of Biochemistry, Год журнала: 2024, Номер 93(1), С. 79 - 108

Опубликована: Апрель 10, 2024

DEAD- and DExH-box ATPases (DDX/DHXs) are abundant highly conserved cellular enzymes ubiquitously involved in RNA processing. By remodeling RNA–RNA RNA–protein interactions, they often function as gatekeepers that control the progression of diverse maturation steps. Intriguingly, most DDX/DHXs localize to membraneless organelles (MLOs) such nucleoli, nuclear speckles, stress granules, or processing bodies. Recent findings suggest not only localization MLOs can promote interaction between their targets but also key regulators MLO formation turnover through condensation ATPase activity.In this review, we describe molecular ribosome biogenesis, messenger splicing, export, translation, storage decay well association with prominent MLOs. We discuss how enzymatic is linked DDX/DHX condensation, accumulation ribonucleoprotein particles dynamics. Future research will reveal these processes orchestrate life cycle space time.

Язык: Английский

Процитировано

9
A comprehensive review on DDX3X liquid phase condensation in health and neurodevelopmental disorders DOI
Ivan Rosa e Silva, Juliana Helena Costa Smetana, Juliana Ferreira de Oliveira

и другие.

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 259, С. 129330 - 129330

Опубликована: Янв. 11, 2024

Язык: Английский

Процитировано

6
Interferon-α stimulates DExH-box helicase 58 to prevent hepatocyte ferroptosis DOI Creative Commons

Kaiwei Jia,

Ren-qi Yao, Yiwen Fan

и другие.

Military Medical Research, Год журнала: 2024, Номер 11(1)

Опубликована: Апрель 15, 2024

Liver ischemia/reperfusion (I/R) injury is usually caused by hepatic inflow occlusion during liver surgery, and frequently observed war wounds trauma. Hepatocyte ferroptosis plays a critical role in I/R injury, however, it remains unclear whether this process controlled or regulated members of the DEAD/DExH-box helicase (DDX/DHX) family. The expression DDX/DHX family was screened using transcriptome analysis. Hepatocyte-specific Dhx58 knockout mice were constructed, partial operation performed. Single-cell RNA sequencing (scRNA-seq) post suggested enhanced Dhx58hep-/-. mRNAs proteins associated with DExH-box 58 (DHX58) immunoprecipitation-sequencing (RIP-seq) IP-mass spectrometry (IP-MS). Excessive production reactive oxygen species (ROS) decreased IFN-stimulated gene hepatocytes promoted ferroptosis, while treatment IFN-α increased DHX58 prevented injury. Mechanistically, RNA-binding activity constitutively associates mRNA glutathione peroxidase 4 (GPX4), central suppressor, recruits m6A reader YT521-B homology domain containing 2 (YTHDC2) to promote translation Gpx4 an m6A-dependent manner, thus enhancing GPX4 protein levels preventing ferroptosis. This study provides mechanistic evidence that stimulates m6A-modified mRNA, suggesting potential clinical application prevention

Язык: Английский

Процитировано

6
Phase separation in cancer at a glance DOI Creative Commons

Qingqing Xie,

Jiejuan Cheng,

Wuxuan Mei

и другие.

Journal of Translational Medicine, Год журнала: 2023, Номер 21(1)

Опубликована: Апрель 1, 2023

Abstract Eukaryotic cells are segmented into multiple compartments or organelles within the cell that regulate distinct chemical and biological processes. Membrane-less membrane-less microscopic cellular contain protein RNA molecules perform a wide range of functions. Liquid–liquid phase separation (LLPS) can reveal how develop via dynamic biomolecule assembly. LLPS either segregates undesirable from aggregates desired ones in cells. Aberrant results production abnormal biomolecular condensates (BMCs), which cause cancer. Here, we explore intricate mechanisms behind formation BMCs its biophysical properties. Additionally, discuss recent discoveries related to tumorigenesis, including aberrant signaling transduction, stress granule formation, evading growth arrest, genomic instability. We also therapeutic implications Understanding concept mechanism role tumorigenesis is crucial for antitumor strategies.

Язык: Английский

Процитировано

15
DEAD/DEAH-box RNA helicases shape the risk of neurodevelopmental disorders DOI

Chiara Fiorenzani,

Adele Mossa,

Silvia De Rubeis

и другие.

Trends in Genetics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
The DEAD-box helicase eIF4A1/2 acts as RNA chaperone during mitotic exit enabling chromatin decondensation DOI Creative Commons
Ramona Jühlen,

Sabine C. Wiesmann,

Anja Scheufen

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 11, 2025

Abstract During mitosis, chromosomes condense and decondense to segregate faithfully undamaged. The exact molecular mechanisms are not well understood. We identify the DEAD-box helicase eIF4A1/2 as a critical factor in this process. In cell-free condensation assay is crucial for process, relying on its RNA-binding ability but ATPase activity. Reducing levels cells consistently slows down chromatin decondensation during nuclear reformation. Conversely, increasing concentration mitotic accelerates their decondensation. absence of affects perichromatin layer, which surrounds mitosis consists RNA mainly nucleolar proteins. vitro, acts an chaperone, dissociating biomolecular condensates chaperone activity required regulate composition fluidity dynamic reorganization exit mitosis.

Язык: Английский

Процитировано

0
G-Quadruplexes in Nuclear Biomolecular Condensates DOI Open Access
Iuliia Pavlova, Mikhail Iudin, A. V. Surdina

и другие.

Genes, Год журнала: 2023, Номер 14(5), С. 1076 - 1076

Опубликована: Май 13, 2023

G-quadruplexes (G4s) have long been implicated in the regulation of chromatin packaging and gene expression. These processes require or are accelerated by separation related proteins into liquid condensates on DNA/RNA matrices. While cytoplasmic G4s acknowledged scaffolds potentially pathogenic condensates, possible contribution to phase transitions nucleus has only recently come light. In this review, we summarize growing evidence for G4-dependent assembly biomolecular at telomeres transcription initiation sites, as well nucleoli, speckles, paraspeckles. The limitations underlying assays remaining open questions outlined. We also discuss molecular basis apparent permissive role vitro condensate based interactome data. To highlight prospects risks G4-targeting therapies with respect transitions, touch upon reported effects G4-stabilizing small molecules nuclear condensates.

Язык: Английский

Процитировано

10
Me31B: a key repressor in germline regulation and beyond DOI Open Access
Ming Gao

Bioscience Reports, Год журнала: 2024, Номер 44(5)

Опубликована: Апрель 12, 2024

Maternally Expressed at 31B (Me31B), an evolutionarily conserved ATP-dependent RNA helicase, plays important role in the development of germline across diverse animal species. Its cellular functionality has been posited as a translational repressor, participating various metabolism pathways to intricately regulate spatiotemporal expression RNAs. Despite its evident significance, precise and mechanistic underpinnings Me31B remain insufficiently understood. This article endeavors comprehensively review historic recent research on Me31B, distill major findings, discern generalizable patterns Me31B's functions different contexts, provide insights into fundamental mechanism action. The primary focus this centers elucidating Drosophila within germline, while concurrently delving pertinent orthologs other species systems.

Язык: Английский

Процитировано

3