Neuroscience Bulletin, Год журнала: 2025, Номер unknown
Опубликована: Янв. 7, 2025
Язык: Английский
Journal of Biological Chemistry, Год журнала: 2024, Номер 300(5), С. 107269 - 107269
Опубликована: Апрель 6, 2024
Coenzyme Q10 (CoQ
Язык: Английский
Процитировано
4
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Апрель 25, 2025
Coenzyme Q10 (Q10) plays a critical role in cellular energy conversion within the mitochondrial respiratory chain and offers protective effects against oxidative metabolic stress. In this study, we investigated impact of on spatio-temporal patterns energetics keratinocyte-derived HaCaT cells, utilizing genetically-encoded FRET sensor AMPfret. Engineered from AMP-activated protein kinase (AMPK), leverages endogenous affinities that evolved to detect stress, specifically decreases ATP/ADP ATP/AMP ratios pose threat cell survival. We successfully established cells stably expressing AMPfret, validated their functionality by inducing stress with 2-deoxy-D-glucose, demonstrated Q10, together high glucose conditions culture, can enhance compared low controls. then employed AMPfret analyze response keratinocytes Luperox (tert-butyl peroxide), potent organic prooxidant, presence varying intracellular levels Q10. Preloading was protective, slowing speed reducing extend response. contrast, preincubation Simvastatin, an inhibitor mevalonate biosynthesis pathway, depleted levels, accelerated onset led early death as Under all conditions, revealed cell-to-cell heterogeneity at baseline Luperox. Overall, tracking changes state time single-cell level allows further insights into beneficial enhancing bioenergetics skin potential AMPK mediating responses altered levels.
Язык: Английский
Процитировано
0
Annals of Clinical and Translational Neurology, Год журнала: 2024, Номер 11(4), С. 1067 - 1074
Опубликована: Март 4, 2024
Abstract Biallelic mutations in the coenzyme Q7 (COQ7) encoding gene were recently identified as a genetic cause of distal hereditary motor neuropathy. Here, we explored clinical, electrophysiological, pathological, and characteristics Chinese patient with spastic paraplegia associated recessive variants COQ7 . This carried novel c.322C>A (p.Pro108Thr) homozygous variant. Sural biopsy revealed mild mixed axonal demyelinating degeneration. Immunoblotting showed significant decrease protein level patient's fibroblasts. study confirmed that variant HSP, further extended to phenotypic spectrum ‐related disorders.
Язык: Английский
Процитировано
3
Obstetrics Gynecology and Reproduction, Год журнала: 2024, Номер 18(5), С. 720 - 734
Опубликована: Авг. 13, 2024
Maternal mitochondria provide energy to the embryo through oxidative phosphorylation before blastocyst implantation, where intracellular is mainly supplied by glycolysis. Thus, it obvious that play a crucial role in providing for embryogenesis. Coenzyme Q10 (CoQ10) powerful endogenous membrane-localized antioxidant protects circulating lipoproteins from lipid peroxidation. The results of several recent clinical studies have shown exogenous CoQ10 supplements exert effects and may be potential therapy reduce stress. deficiency increases risk impaired embryonic development; however, this relationship remains unclear. Given level influenced enzymes involved its synthesis, difficult say whether disorders are caused or directly result defects target gene. It has been absence CoQ10, ATP synthesis decreases parallel with increased stress mitochondria, two biological events which affect development. review highlights importance as an improving egg quality, also emphasizes key necessary conduct further aimed at studying metabolic changes during embryogenesis, well mechanism effects.
Язык: Английский
Процитировано
3
Neuroscience Bulletin, Год журнала: 2025, Номер unknown
Опубликована: Янв. 7, 2025
Язык: Английский
Процитировано
0