Uncovering the Mechanisms: The Role of Biotrophic Fungi in Activating or Suppressing Plant Defense Responses DOI Creative Commons
Michel Leiva-Mora, Yanelis Capdesuñer, Ariel Villalobos-Olivera

и другие.

Journal of Fungi, Год журнала: 2024, Номер 10(9), С. 635 - 635

Опубликована: Сен. 5, 2024

This paper discusses the mechanisms by which fungi manipulate plant physiology and suppress defense responses producing effectors that can target various host proteins. Effector-triggered immunity effector-triggered susceptibility are pivotal elements in complex molecular dialogue underlying plant-pathogen interactions. Pathogen-produced effector molecules possess ability to mimic pathogen-associated patterns or hinder binding of pattern recognition receptors. Effectors directly nucleotide-binding domain, leucine-rich repeat receptors, downstream signaling components defense. Interactions between these receptor-like kinases plants critical this process. Biotrophic adeptly exploit networks key hormones, including salicylic acid, jasmonic abscisic ethylene, establish a compatible interaction with their hosts. Overall, highlights importance understanding interplay fungal develop effective strategies for disease management.

Язык: Английский

Reversible ubiquitination conferred by domain shuffling controls paired NLR immune receptor complex homeostasis in plant immunity DOI Creative Commons

Zhiyi Chen,

Jianhua Huang, Jian-Yu Li

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 26, 2025

Plant intracellular NLR immune receptors can function individually or in pairs to detect pathogen effectors and activate responses. homeostasis has be tightly regulated ensure proper defense without triggering autoimmunity. However, contrast singleton NLRs, the mechanisms controlling paired NLRs complex are less understood. The Arabidopsis RRS1/RPS4 receptor confers disease resistance through effector recognition mediated by integrated WRKY domain of RRS1. Here, proximity labeling, we reveal a ubiquitination-deubiquitination cycle that controls complex. E3 ligase RARE directly binds ubiquitinates RRS1's promote its proteasomal degradation, thereby destabilizing RPS4 indirectly compromising stability Conversely, deubiquitinating enzymes UBP12/UBP13 deubiquitinate domain, counteracting RARE's effects. Interestingly, abundance transcription factors WRKY70 WRKY41 is also UBP12/UBP13. Phylogenetic analysis suggests this regulation likely transferred from WRKY70/WRKY41 RRS1 upon integration. Our findings improve our understanding homeostatic uncover paradigm whereby integration co-opt preexisting post-translational modification regulate novel protein functions. plant needs avoid authors revealed shuffling transfer pre-existing ubiquitination regulatory mechanism control harboring domains.

Язык: Английский

Процитировано

0
Filamentation activates bacterial Avs5 antiviral protein DOI Creative Commons
Yiqun Wang, Yuqing Tian, Xu Yang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 11, 2025

Bacterial antiviral STANDs (Avs) are evolutionarily related to the nucleotide-binding oligomerization domain (NOD)-like receptors widely distributed in immune systems across animals and plants. EfAvs5, a type 5 Avs from Escherichia fergusonii, contains an N-terminal SIR2 effector domain, NOD, C-terminal sensor conferring protection against diverse phage invasions. Despite established roles of STAND prokaryotic eukaryotic immunity, mechanism underlying their collaboration remains unclear. Here we present cryo-EM structures EfAvs5 filaments, elucidating mechanisms dimerization, filamentation, filament bundling, ATP binding, NAD+ hydrolysis, all which crucial for anti-phage defense. The NOD domains engage intra- inter-dimer interaction form individual filament, while outward contribute bundle formation. Filamentation potentially stabilizes dimeric configuration, thereby activating NADase activity EfAvs5. Furthermore, identify nucleotide kinase gp1.7 T7 as activator demonstrating its ability induce filamentation activity. Together, uncover assembly Avs5 unique switch enzyme activities perform defenses. protect Here, authors reveal that forms clustered filaments hydrolysis can be activated by protein, mechanism.

Язык: Английский

Процитировано

0
Utilizing Plant Synthetic Biology to Accelerate Plant-Microbe Interactions Research DOI Creative Commons
Xiaohan Yang, Joanna Tannous, Tomás A. Rush

и другие.

BioDesign Research, Год журнала: 2025, Номер unknown, С. 100007 - 100007

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
MIR396d-p3 Negatively Regulates Apple Resistance to Colletotrichum gloeosporioides via MdUGT89A2 and MdRGA3 DOI Creative Commons
B. X. Zhang, Jinqi Tang, Zhirui Ji

и другие.

Horticulturae, Год журнала: 2025, Номер 11(4), С. 351 - 351

Опубликована: Март 25, 2025

Apple (Malus domestica) is an economically important fruit crop, but its production affected by Glomerella leaf spot, a devastating disease caused the fungal pathogen Colletotrichum gloeosporioides. MicroRNA (miRNA) kind of non-coding RNA that plays role in process plant–pathogen interactions. However, little known about miRNAs influence apple resistance against C. A novel miRNA, MIR396d-p3, was identified through small sequencing (sRNA-seq). Functional analyses revealed MIR396d-p3 negatively regulates to In addition, MdUGT89A2 and MdRGA3 were confirmed as targets using 5′ RACE heterologous expression assays. We further found overexpressing induce gloeosporioides, while silencing reduces These results indicate response infection gloeosporioides regulating expressions MdRGA3. This research provides new perspective on interaction between apples offers possible for breeding.

Язык: Английский

Процитировано

0
Bacterial XopR subverts RIN4 complex-mediated plant immunity via plasma membrane-associated percolation DOI
Xinlu Zhu,

Weibing Wang,

Simou Sun

и другие.

Developmental Cell, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Identification and expression of the peach TNL RMia genes for the resistance to the root-knot nematode Meloidogyne incognita DOI Creative Commons
Henri Duval, Laure Heurtevin, Naïma Dlalah

и другие.

Scientia Horticulturae, Год журнала: 2025, Номер 343, С. 114081 - 114081

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Dynamic regulation of rice immunity by a canonical protein complex DOI
Xingyu Zhang, Yu Wang, Xuemin Zhou

и другие.

Science Bulletin, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome DOI Creative Commons
Muniyandi Selvaraj, AmirAli Toghani, Hsuan Pai

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 18, 2023

Abstract Nucleotide-binding domain and leucine-rich repeat (NLR) proteins can engage in complex interactions to detect pathogens execute a robust immune response via downstream helper NLRs. However, the biochemical mechanisms of NLR activation by upstream sensor NLRs remain poorly understood. Here, we show that coiled-coil NRC2 accumulates vivo as homodimer converts into higher order oligomer upon its virus disease resistance protein Rx. The Cryo-EM structure resting state revealed intermolecular mediate formation. These dimerization interfaces have diverged between paralogous NRC insulate critical network nodes enable redundant pathways. Our results expand molecular pointing transition from homodimers higher-order oligomeric resistosomes.

Язык: Английский

Процитировано

10
Multiple Chitin- or Avirulent Strain-Triggered Immunity Induces Microbiome Reassembly in Rice DOI Creative Commons

Sauban Musa Jibril,

Chun Wang, Chao Yang

и другие.

Microorganisms, Год журнала: 2024, Номер 12(7), С. 1323 - 1323

Опубликована: Июнь 28, 2024

Magnaporthe oryzae is one of the most important fungal pathogens rice. Chitin and avirulent strains can induce two layers immunity response, pathogen-associated molecular pattern (PAMP)-triggered (PTI) effector-triggered (ETI), in rice with cognate R genes. However, little known about assembly microbiome induced by PTI ETI In this study, we investigate impact continuous treatment M. strain AvrPi9 chitin on bacterial endophytic community varieties harboring resistant gene Pi9 their antagonistic activity against blast fungus. Analysis 16S rRNA showed a significant increase diversity microbial co-occurrence network complexity number beneficial taxa—Bacillus, Pseudomonas, Microbacterium, Stenotrophomonas spp.—following treatments. The antifungal assay endophytes recovered from leaves few bacteria potential treated strains, suggesting that sequential decreased oryzae. Moreover, identified Bacillus safensis Ch_66 altitudinis Nc_68 overall activities vivo vitro. Our findings provide novel insight into response to different innate reactions.

Язык: Английский

Процитировано

2
Ubiquitination and degradation of plant helper NLR by the Ralstonia solanacearum effector RipV2 overcome tomato bacterial wilt resistance DOI Creative Commons
Peipei Qi, Dan Zhang, Ying Zhang

и другие.

Cell Reports, Год журнала: 2024, Номер 43(8), С. 114596 - 114596

Опубликована: Авг. 1, 2024

The Ralstonia solanacearum species complex causes bacterial wilt in a variety of crops. Tomato cultivar Hawaii 7996 is widely used resistance resource; however, the evaded by virulent strains, with underlying mechanisms still unknown. Here, we report that phylotype Ⅱ strain ES5-1 can overcome resistance. RipV2, type Ⅲ effector specific to vital overcoming tomato which encodes an E3 ubiquitin ligase, suppresses immune responses and Toll/interleukin-1 receptor/resistance nucleotide-binding/leucine-rich repeat (NLR) (TNL)-mediated cell death. helper NLR N requirement gene 1 (NRG1), enhanced disease susceptibility (EDS1), senescence-associated 101b (SAG101b) are identified as RipV2 target proteins. essential for virulence but not SlNRG1-silenced tomato, demonstrating SlNRG1 be target. Our results dissect disrupting immunity highlight importance converged components conferring

Язык: Английский

Процитировано

2