Clinical Reviews in Allergy & Immunology,
Год журнала:
2024,
Номер
68(1)
Опубликована: Дек. 27, 2024
Asthma
is
a
chronic
airway
inflammatory
disease
that
affects
millions
globally.
Although
glucocorticoids
are
mainstay
of
asthma
treatment,
subset
patients
show
resistance
to
these
therapies,
resulting
in
poor
control
and
increased
morbidity.
The
complex
mechanisms
underlying
steroid-resistant
(SRA)
involve
Th1
Th17
lymphocyte
activity,
neutrophil
recruitment,
NLRP3
inflammasome
activation.
Recent
studies
provided
evidence
innate
lymphoid
cells
type
3
(ILC3s)
might
be
potential
therapeutic
targets
for
non-eosinophilic
(NEA)
SRA.
Like
cells,
ILC3s
play
crucial
roles
immune
responses,
inflammation,
tissue
homeostasis,
contributing
severity
corticosteroid
NEA.
Biologics
targeting
ILC3-related
pathways
have
shown
promise
managing
Th2-low
asthma,
suggesting
new
avenues
SRA
treatment.
This
review
aims
explore
the
risk
factors
SRA,
discuss
challenges
consolidate
current
findings
on
elucidate
their
role
respiratory
conditions.
We
present
latest
involvement
human
diseases
development.
Furthermore,
we
emerging
biologics
NEA
highlights
challenges,
strategies,
addresses
significant
gap
research,
with
implications
improving
management
asthma.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Ноя. 14, 2024
Abstract
In
the
last
decade,
messenger
ribonucleic
acid
(mRNA)-based
drugs
have
gained
great
interest
in
both
immunotherapy
and
non-immunogenic
applications.
This
surge
can
be
largely
attributed
to
demonstration
of
distinct
advantages
offered
by
various
mRNA
molecules,
alongside
rapid
advancements
nucleic
delivery
systems.
It
is
noteworthy
that
immunogenicity
presents
a
double-edged
sword.
context
immunotherapy,
extra
supplementation
adjuvant
generally
required
for
induction
robust
immune
responses.
Conversely,
non-immunotherapeutic
scenarios,
activation
unwanted
considering
host
tolerability
high
expression
demand
mRNA-encoded
functional
proteins.
Herein,
mainly
focused
on
linear
non-replicating
mRNA,
we
overview
preclinical
clinical
progress
prospects
medicines
encompassing
vaccines
other
therapeutics.
We
also
highlight
importance
focusing
host-specific
variations,
including
age,
gender,
pathological
condition,
concurrent
medication
individual
patient,
maximized
efficacy
safety
upon
administration.
Furthermore,
deliberate
potential
challenges
may
encounter
realm
disease
treatment,
current
endeavors
improvement,
as
well
application
future
advancements.
Overall,
this
review
aims
present
comprehensive
understanding
mRNA-based
therapies
while
illuminating
prospective
development
drugs.
Communications Biology,
Год журнала:
2025,
Номер
8(1)
Опубликована: Янв. 11, 2025
Pseudouridine
(Ψ)
is
an
abundant
RNA
chemical
modification
that
plays
critical
biological
functions.
Current
Ψ
detection
methods
are
limited
in
identifying
Ψs
at
base-resolution
U-rich
sequence
contexts,
where
occurs
frequently.
Here
we
report
"Mut-Ψ-seq"
utilizes
the
classic
N-cyclohexyl
N′-(2-morpholinoethyl)carbodiimide
(CMC)
agent
and
evolved
reverse
transcriptase
("RT-1306")
for
mapping
base-resolution.
CMC
selectively
labels
forming
CMC-Ψ
adduct
show
RT-1306
presents
promoted
read-through
mutation
against
CMC-Ψ.
We
a
high-confidence
list
of
sites
polyA-enriched
RNAs
from
HEK-293T
cells
identified
by
orthogonal
treatments
(CMC
bisulfite).
The
signatures
resolve
position
UU-containing
sequences,
revealing
diverse
occurrence
such
sequences.
This
work
provides
datasets
research
Ψ,
expands
toolkit
epitranscriptomic
studies
combining
engineering
selective
labeling
strategies.
Mut-Ψ-seq
deploys
chemistry
to
ambiguity
UU
contexts
reports
high-confident
human
transcriptome
methods.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 1, 2024
Understanding
the
interactions
between
different
RNA
modifications
is
essential
for
unraveling
their
biological
functions.
Here,
we
report
NanoPsiPy,
a
computational
pipeline
that
employs
nanopore
direct
sequencing
to
identify
pseudouridine
(Ψ)
sites
and
quantify
levels
at
single-nucleotide
resolution.
We
validated
NanoPsiPy
by
transcriptome-wide
profiling
of
PUS7-dependent
Ψ
in
poly-A
rRNA.
leverages
Ψ-induced
U-to-C
basecalling
errors
data,
allowing
detection
both
low
high
stoichiometric
sites.
identified
8,624
1,246
mRNAs
encoding
proteins
associated
with
ribosome
biogenesis,
translation,
energy
metabolism.
Importantly,
integrative
analysis
revealed
PUS7
knockdown
increases
global
mRNA
N
Acta Biochimica et Biophysica Sinica,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 1, 2024
The
intricate
world
of
RNA
modifications,
collectively
termed
the
epitranscriptome,
covers
over
170
identified
modifications
and
impacts
metabolism
and,
consequently,
almost
all
biological
processes.
In
this
review,
we
focus
on
regulatory
roles
functions
a
panel
dominant
(including
m
Amino
acids
are
the
most
critical
active
compounds
in
organisms,
can
exist
D-
and
L-enantiomeric
forms.
Recently,
research
revealed
that
D-amino
essential
to
human
health.
This
study
was
first
investigate
acid
level
food
drug
elucidated
mechanism
of
action
on
mouse
tissues
serum.
The
results
showed
were
detected
all
selected
products,
among
which
D-aspartic
acid,
D-histidine,
D-leucine
being
abundant.
After
20
d
feeding
experiment,
D-serine,
D-arginine,
D-ornithine,
D-lysine
significantly
accumulated
mice
tissue
serum
a
dose-dependent
manner.
non-targeted
metabolic
11
categories
metabolites
differences,
with
lipids
lipid-like
substances
showing
differences.
Glycerophospholipid
metabolism
choline
cancer
pathways.
According
findings,
D-AA
might
have
significant
effect
lipid
metabolism.
NAR Genomics and Bioinformatics,
Год журнала:
2025,
Номер
7(1)
Опубликована: Янв. 7, 2025
Small
nucleolar
RNAs
(snoRNAs)
are
one
of
the
most
abundant
and
evolutionary
ancient
group
functional
non-coding
RNAs.
They
were
originally
described
as
guides
post-transcriptional
rRNA
modifications,
but
emerging
evidence
suggests
that
snoRNAs
fulfil
an
impressive
variety
cellular
functions.
To
reveal
true
complexity
snoRNA-dependent
functions,
we
need
to
catalogue
first
complete
repertoire
in
a
given
context.
While
systematic
mapping
characterization
"snoRNAomes"
for
some
species
have
been
recently,
this
has
not
done
hitherto
zebrafish
(Danio
rerio).
Using
size-fractionated
RNA
sequencing
data
from
adult
tissues,
created
interactive
"snoRNAome"
database
species.
Our
custom-designed
analysis
pipeline
allowed
us
identify
with
high-confidence
67
previously
unannotated
genome,
resulting
set
date
Reanalyzing
multiple
published
datasets,
also
provide
dynamic
expression
during
early
stages
development
tissue-specific
patterns
others
adults.
facilitate
further
investigations
into
functions
zebrafish,
novel
database,
snoDanio,
which
can
be
used
explore
small
transcriptomic
data.
