Genomic Functional Analysis and Cellulase Characterization for the Enzyme-Producing Strain Bacillus subtilis Y4X3 Isolated from Saline–Alkaline Soil in Xinjiang, China

Microorganisms, Год журнала: 2025, Номер 13(3), С. 552 - 552

Опубликована: Фев. 28, 2025

Biotechnological research and application of microbial enzyme production have consistently been focal points for scientific inquiry industrial advancement. In this study, Bacillus subtilis Y4X3 was isolated from saline–alkaline soil in Xinjiang, China. Extracellular analysis revealed that B. can secrete various enzymes, including cellulase, xylanase, protease, amylase. Sequencing assembly the complete genome strain a size 4,215,636 bp with 43.51% C + G content, 4438 coding genes. Genome annotation performed databases to predict gene functions Y4X3, variety genes related carbohydrate metabolism were identified. A cellulase-encoding subsequently cloned heterologously expressed Escherichia coli. The optimum pH temperature purified cellulase Cel5A 5.0 60 °C, respectively. Stability remained stable at 5.0–9.0 20–60 °C; after 1 h 9.0, relative activity still exceeded 60%. Additionally, positively affected by metal ions exhibited good tolerance multiple chemical reagents. results indicate has potential produce enzymes could serve as promising candidate more efficient cost-effective applications; characterized thermostable alkali-resistant also applications biotechnology industry.

Язык: Английский

Deaminase-Driven Random Mutation Enables Efficient DNA Mutagenesis for Protein Evolution

Chemical Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Protein evolution has emerged as a crucial tool for generating proteins with novel characteristics. A key step in protein is the mutagenesis of protein-coding DNA. Error-prone PCR (epPCR) is...

Язык: Английский