The thylakoid- and pyrenoid-localized phosphate transporter PHT4-9 is essential for photosynthesis in Chlamydomonas DOI Creative Commons

Kashif M. Shaikh,

Charlotte E. Walker, Dávid Tóth

и другие.

PLANT PHYSIOLOGY, Год журнала: 2025, Номер 198(1)

Опубликована: Апрель 24, 2025

ABSTRACT Phosphate (Pi) is essential for photosynthesis in the chloroplast of algae and plants. Pi homeostasis maintained by transporters from several families, whose identities are largely unknown as compared with land Here, we assess role putative PHOSPHATE TRANSPORTER 4-9 Chlamydomonas reinhardtii (CrPHT4-9) maintaining modulating photosynthesis. Based on phylogenetic analyses heterologous expression a yeast (Saccharomyces cerevisiae) strain lacking transporters, demonstrate that CrPHT4-9 transporter closely related to members PHT4 family Arabidopsis (Arabidopsis thaliana). localized within chloroplast, more specifically thylakoid membrane network tubules traversing CO2-fixing pyrenoid. Two mutants (Crpht4-9) exhibit defective photoautotrophic growth, altered cell morphology ultrastructure under CO2-limiting conditions. In Crpht4-9 mutants, further show an increased proton motive force across membrane, enhanced energy- state-transition-dependent non-photochemical quenching chlorophyll fluorescence, diminished photosynthetic electron transport ATP synthase activity. The reduced affinity inorganic carbon, indicating impaired carbon-concentrating mechanism. These phenotypes recovered genetic complementation well ample CO2 supply and, interestingly, deprivation. Therefore, conclude thylakoid- pyrenoid-localized maintains growth.

Язык: Английский

The thylakoid- and pyrenoid-localized phosphate transporter PHT4-9 is essential for photosynthesis in Chlamydomonas DOI Creative Commons

Kashif M. Shaikh,

Charlotte E. Walker, Dávid Tóth

и другие.

PLANT PHYSIOLOGY, Год журнала: 2025, Номер 198(1)

Опубликована: Апрель 24, 2025

ABSTRACT Phosphate (Pi) is essential for photosynthesis in the chloroplast of algae and plants. Pi homeostasis maintained by transporters from several families, whose identities are largely unknown as compared with land Here, we assess role putative PHOSPHATE TRANSPORTER 4-9 Chlamydomonas reinhardtii (CrPHT4-9) maintaining modulating photosynthesis. Based on phylogenetic analyses heterologous expression a yeast (Saccharomyces cerevisiae) strain lacking transporters, demonstrate that CrPHT4-9 transporter closely related to members PHT4 family Arabidopsis (Arabidopsis thaliana). localized within chloroplast, more specifically thylakoid membrane network tubules traversing CO2-fixing pyrenoid. Two mutants (Crpht4-9) exhibit defective photoautotrophic growth, altered cell morphology ultrastructure under CO2-limiting conditions. In Crpht4-9 mutants, further show an increased proton motive force across membrane, enhanced energy- state-transition-dependent non-photochemical quenching chlorophyll fluorescence, diminished photosynthetic electron transport ATP synthase activity. The reduced affinity inorganic carbon, indicating impaired carbon-concentrating mechanism. These phenotypes recovered genetic complementation well ample CO2 supply and, interestingly, deprivation. Therefore, conclude thylakoid- pyrenoid-localized maintains growth.

Язык: Английский

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