Frontiers in Plant Science,
Год журнала:
2021,
Номер
12
Опубликована: Май 28, 2021
Agriculture
is
an
important
source
of
human
food.
However,
current
agricultural
practices
need
modernizing
and
strengthening
to
fulfill
the
increasing
food
requirements
growing
worldwide
population.
Genome
editing
(GE)
technology
has
been
used
produce
plants
with
improved
yields
nutritional
value
as
well
higher
resilience
herbicides,
insects,
diseases.
Several
GE
tools
have
developed
recently,
including
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
nucleases,
a
customizable
successful
method.
The
main
steps
process
involve
introducing
transgenes
or
CRISPR
into
via
specific
gene
delivery
systems.
certain
limitations,
time-consuming
complicated
protocols,
potential
tissue
damage,
DNA
incorporation
in
host
genome,
low
transformation
efficiency.
To
overcome
these
issues,
nanotechnology
emerged
groundbreaking
modern
technique.
Nanoparticle-mediated
superior
conventional
biomolecular
approaches
because
it
enhances
efficiency
for
both
temporal
(transient)
permanent
(stable)
genetic
modifications
various
plant
species.
discoveries
advanced
technologies,
challenges
developing
short-term
breeding
strategy
remain.
Thus,
this
review,
nanobased
systems
engineering
are
discussed
detail.
Moreover,
we
suggested
effective
method
hasten
crop
improvement
programs
by
combining
such
speed
CRISPR/Cas,
nanotechnology.
overall
aim
review
provide
detailed
overview
nanotechnology-based
techniques
suggest
applications
possible
enhancement.
Annual Review of Plant Biology,
Год журнала:
2019,
Номер
70(1), С. 667 - 697
Опубликована: Март 5, 2019
Enhanced
agricultural
production
through
innovative
breeding
technology
is
urgently
needed
to
increase
access
nutritious
foods
worldwide.
Recent
advances
in
CRISPR/Cas
genome
editing
enable
efficient
targeted
modification
most
crops,
thus
promising
accelerate
crop
improvement.
Here,
we
review
CRISPR/Cas9
and
its
variants
examine
their
applications
plant
related
manipulations.
We
highlight
base-editing
tools
that
nucleotide
substitutions
describe
the
various
delivery
systems,
particularly
DNA-free
methods,
have
linked
with
breeding.
summarize
of
for
trait
improvement,
development
techniques
fine-tuning
gene
regulation,
strategies
virus
resistance,
use
high-throughput
mutant
libraries.
outline
future
perspectives
synthetic
biology
domestication,
specificity,
homology-directed
repair,
drives.
Finally,
discuss
challenges
opportunities
precision
bright
agriculture.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2019,
Номер
7
Опубликована: Май 29, 2019
This
mini-review
aims
at
gaining
knowledge
on
basic
aspects
of
plant
nanotechnology.
While
in
recent
years
the
enormous
progress
nanotechnology
biomedical
sciences
has
revolutionized
therapeutic
and
diagnostic
approaches,
comprehension
nanoparticle-plant
interactions,
including
uptake,
mobilization
accumulation,
is
still
its
infancy.
Deeper
studies
are
needed
to
establish
impact
nanomaterials
(NMs)
growth
agro-ecosystems,
develop
smart
applications
crop
improvement.
Herein
we
provide
a
short
overview
NMs
employed
science
concisely
describe
key
NM-plant
interactions
terms
mechanisms
biological
effects.
The
major
current
plants
reviewed
also
discussing
potential
use
polymeric
soft
which
may
open
new
safer
opportunities
for
delivery
biomolecules
strategies
genetic
engineering,
with
final
aim
enhance
defense
and/or
stimulate
development
and,
ultimately,
production.
Finally,
envisage
that
multidisciplinary
collaborative
approaches
will
be
central
fill
gap
push
towards
agriculture
more
general,
research.
National Science Review,
Год журнала:
2019,
Номер
6(3), С. 421 - 437
Опубликована: Янв. 15, 2019
The
clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)-associated
protein
9
(Cas9)
genome
editing
system
is
a
powerful
tool
for
targeted
gene
modifications
in
wide
range
of
species,
including
plants.
Over
the
last
few
years,
this
has
revolutionized
way
scientists
perform
genetic
studies
and
crop
breeding,
due
to
its
simplicity,
flexibility,
consistency
high
efficiency.
Considerable
progress
been
made
optimizing
CRISPR/Cas9
systems
plants,
particularly
mutagenesis.
However,
there
are
still
number
important
challenges
ahead,
methods
efficient
delivery
CRISPR
other
tools
most
more
effective
strategies
sequence
knock-ins
replacements.
We
provide
our
viewpoint
on
goals,
potential
concerns
future
development
application
plant
tools.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2020,
Номер
8
Опубликована: Фев. 18, 2020
The
main
challenges
in
drug
delivery
systems
are
to
protect,
transport
and
release
biologically
active
compounds
at
the
right
time
a
safe
reproducible
manner,
usually
specific
target
site.
In
past,
nano-carriers
have
contributed
development
of
precision
medicine
lesser
extent
focused
on
its
inroads
agriculture.
concept
engineered
may
be
promising
route
address
confounding
agriculture
that
could
perhaps
lead
an
increase
crop
production
while
reducing
environmental
impact
associated
with
protection
food
production.
objective
this
review
is
contrast
advantages
disadvantages
different
types
nanoparticles
currently
used
biomedical
field
along
their
fabrication
methods
discuss
potential
use
these
technologies
larger
scale
Here
we
explain
what
problem
nano-delivery
intent
solve
as
technological
platform
describe
benefits
technology
has
brought
medicine.
Also
here
highlight
drawbacks
face
during
translation
agricultural
applications,
based
lessons
learned
so
far
from
for
purposes.
We
not
only
characteristics
ideal
system,
but
also
constraints
regarding
including
technical,
environmental,
legal
aspects.
A
key
motivation
evaluate
agriculture,
especially
area
plant
breeding,
growth
promotion,
disease
control,
post-harvest
quality
control.
Further,
importance
rational
design
identify
current
research
gaps
enable
scale-up
relevant
applications
treatment
diseases,
controlled
fertilizers,
breeding.
