An Insight in Developing Carrier-Free Immobilized Enzymes

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Март 2, 2022

Enzymes play vital roles in all organisms. The enzymatic process is progressively at its peak, mainly for producing biochemical products with a higher value. immobilization of enzymes can sometimes tremendously improve the outcome biocatalytic processes, making product(s) relatively pure and economical. Carrier-free immobilized increase yield product stability enzyme biocatalysis. Immobilized are easier to purify. Due these varied advantages, researchers tempted explore carrier-free methods used enzymes. In this review article, we have discussed various aspects immobilization, approaches followed design an advantages disadvantages common processes immobilization.

Язык: Английский

---

A Statistical Analysis of the Sequence and Structure of Thermophilic and Non-Thermophilic Proteins

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(17), С. 10116 - 10116

Опубликована: Сен. 4, 2022

Thermophilic proteins have various practical applications in theoretical research and industry. In recent years, the demand for thermophilic on an industrial scale has been increasing; therefore, engineering of become a hot direction field protein engineering. However, exact mechanism thermostability is not yet known, knowing basis necessary. order to understand proteins, we made statistical analysis sequences, secondary structures, hydrogen bonds, salt bridges, DHA (Donor–Hydrogen–Accepter) angles, bond lengths ten pairs their non-thermophilic orthologous. Our findings suggest that polar amino acids contribute by forming bonds bridges which provide resistance against denaturation. Short length wider angle greater stability proteins. Moreover, increased frequency aromatic contributes thermal more interactions. Additionally, coil, helix, loop structure also thermostability.

Язык: Английский

---

A Dynamic Defect Generation Strategy for Efficient Enzyme Immobilization in Robust Metal‐Organic Frameworks for Catalytic Hydrolysis and Chiral Resolution

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(20)

Опубликована: Март 14, 2023

Enzyme immobilization has been demonstrated to be a favorable protocol for promoting the industrialization of bioactive molecules, but still with formidable challenge. Addressing this challenge, we create dynamic defect generation strategy enzyme by using dissociation equilibrium metal-organic frameworks (MOFs) mediated enzymes. Enzymes can act as "macro ligands" generate competitive coordination against original ligands, along release metal clusters MOFs defects, hence gradual transport enzymes from surface inside. Various efficiently immobilized in afford composites good enzymatic activities, protective performances and exceptional reusabilities. Moreover, multienzyme bioreactors capable efficient cascade reactions also generated. This study provides new opportunities construct highly biocatalysts incorporating different types

Язык: Английский

---

Self-adaptive Metal–Organic Framework Assembles Di-iron Active Sites to Mimic Monooxygenases

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(15), С. 8647 - 8655

Опубликована: Апрель 6, 2023

Despite significant efforts, it remains a challenge to design artificial enzymes that can mimic both structures and functions of natural enzymes. Here, we report the post-synthetic construction binuclear iron catalysts in MOF-253 di-iron monooxygenases. The adjacent bipyridyl (bpy) linkers freely rotate form [(bpy)FeIII(μ2-OH)]2 active site self-adaptive fashion. composition structure sites were characterized by combination inductively coupled plasma-mass spectrometry, thermogravimetric analysis, X-ray absorption Fourier-transform infrared spectroscopy. MOF-based monooxygenase effectively catalyzed oxidative transformations organic compounds, including C-H oxidation alkene epoxidation reactions, using O2 as only oxidant, which indicates successful recapitulation monooxygenases readily accessible MOFs. system exhibited at least 27 times higher catalytic activity than corresponding mononuclear control. DFT calculations showed had 14.2 kcal/mol lower energy barrier rate-determining activation process, suggesting importance cooperativity centers step. stability recyclability also demonstrated.

Язык: Английский

---

Enhanced Bioactivity of Enzyme/MOF Biocomposite via Host Framework Engineering

Journal of the American Chemical Society, Год журнала: 2023, Номер 145(37), С. 20365 - 20374

Опубликована: Сен. 6, 2023

This study reports the successful development of a sustainable synthesis protocol for phase-pure metal azolate framework (MAF-6) and its application in enzyme immobilization. An esterase@MAF-6 biocomposite was synthesized, catalytic performance compared with that esterase@ZIF-8 esterase@ZIF-90 transesterification reactions. Esterase@MAF-6, large pore aperture, showed superior enzymatic to catalyzing reactions using both n-propanol benzyl alcohol as reactants. The hydrophobic nature MAF-6 platform shown activate immobilized esterase into open-lid conformation, which exhibited 1.5- 4-times activity free reaction alcohol, respectively. present work offers insights potential promising matrix immobilization highlights need explore MOF matrices expanded apertures broaden their practical applications biocatalysis.

Язык: Английский

---

Covalent organic framework-immobilized enzymes: A robust engineered catalytic platform for diverse applications

Nano Energy, Год журнала: 2025, Номер unknown, С. 110682 - 110682

Опубликована: Янв. 1, 2025

Язык: Английский

---

An Enhanced Ratiometric Fluorescence Immunosensor for Rapid Detection of AFB₁ Based on the Dual-Enzyme Synergistic Co-Catalytic Effect of Natural Enzymes and Nanozymes

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

Aflatoxin B1 (AFB1) is considered a critical analyte in food safety testing due to its widespread contamination and high toxicity. In this study, ratiometric fluorescent immunosensor with synergistic catalysis by natural enzymes nanozymes (N-N-RF-immunosensor) was constructed for the rapid detection of AFB1. Copper oxide zeolitic imidazolate framework composite nanomaterials (CuO/ZIF-8 NCM) peroxidase oxidase activities were prepared. A fluorescence signal generated through cocatalytic effect CuO/ZIF-8 NCM alkaline phosphatase, response competition interaction between anti-idiotypic nanobody-alkaline phosphatase fusion protein (Nb2-5-AP) The assay could detect aflatoxins within 140 min, exhibiting half maximal inhibitory concentration (IC50) 0.032 ng/mL, limit (LOD) 2.4 pg/mL, linear range 0.0024-1.043 ng/mL. Compared traditional enzyme-linked immunosorbent (ELISA), sensitivity enhanced 25-fold, while parameter shortened 1/3. Furthermore, successfully applied actual samples, recoveries ranging from 81 127%. N-N-RF-immunosensor shows great potential sensitive aflatoxins.

Язык: Английский

---

Recent advances in (chemo)enzymatic cascades for upgrading bio-based resources

Chemical Communications, Год журнала: 2021, Номер 57(82), С. 10661 - 10674

Опубликована: Янв. 1, 2021

Developing (chemo)enzymatic cascades is very attractive for green synthesis, because they streamline multistep synthetic processes.

Язык: Английский

---

Improvement of the enzymatic detoxification activity towards mycotoxins through structure-based engineering

Biotechnology Advances, Год журнала: 2022, Номер 56, С. 107927 - 107927

Опубликована: Фев. 16, 2022

Язык: Английский

---

Recent Progress and Future Prospects of Laccase Immobilization on MOF Supports for Industrial Applications

Applied Biochemistry and Biotechnology, Год журнала: 2023, Номер 196(3), С. 1669 - 1684

Опубликована: Июнь 28, 2023

Язык: Английский

---