Environmental Science & Technology Letters,
Год журнала:
2022,
Номер
9(7), С. 650 - 657
Опубликована: Июнь 17, 2022
The
extensive
production
and
use
of
polyethylene
terephthalate
(PET)
have
generated
an
enormous
amount
plastic
waste,
which
potentially
threatens
the
environment
humans.
Enzyme
biocatalysis
is
a
promising
green
chemistry
alternative,
relative
to
conventional
fossil-derived
process,
achieve
waste
treatment
recycling.
In
this
work,
we
created
biocatalyst,
BIND-PETase,
by
genetically
engineering
curli
Escherichia
coli
cell
with
functional
PETase
enzyme
for
biocatalytic
degradation
PET
plastics.
BIND-PETase
could
degrade
generate
products
at
concentration
level
greater
than
3000
μM
under
various
reaction
conditions.
effects
key
parameters,
including
pH,
temperature,
substrate
mass
load,
surfactant
addition
were
characterized.
was
reusable
remained
stable
no
significant
activity
loss
when
stored
both
4
°C
room
temperature
30
days
(Student's
t
test,
p
>
0.05).
Notably,
enable
microplastics
in
wastewater
effluent
matrix.
Moreover,
depolymerize
highly
crystalline
postconsumer
materials
ambient
conditions
efficiency
9.1%
7
days.
This
study
provides
new
horizon
developing
environmentally
friendly
approaches
solve
recycling
challenge.
Macromolecular Rapid Communications,
Год журнала:
2022,
Номер
43(13)
Опубликована: Май 30, 2022
Today,
plastics
are
ubiquitous
in
everyday
life,
problem
solvers
of
modern
technologies,
and
crucial
for
sustainable
development.
Yet
the
surge
global
demand
growing
world
population
has
triggered
a
tidal
wave
plastic
debris
environment.
Moving
from
linear
to
zero-waste
carbon-neutral
circular
economy
is
vital
future
planet.
Taming
waste
flood
requires
closing
carbon
loop
through
reuse,
mechanical
molecular
recycling,
capture,
use
greenhouse
gas
dioxide.
In
quest
eco-friendly
products,
do
not
need
be
reinvented
but
tuned
reuse
recycling.
Their
full
potential
must
exploited
regarding
energy,
resource,
eco-efficiency,
prevention,
economy,
climate
change
mitigation,
lowering
environmental
pollution.
Biodegradation
holds
promise
composting
bio-feedstock
recovery,
it
neither
Holy
Grail
nor
panacea
littering.
As
an
alternative
downcycling,
recycling
enables
both
closed-loop
recovery
virgin
open-loop
valorization,
producing
hydrogen,
fuels,
refinery
feeds,
lubricants,
chemicals,
carbonaceous
materials.
Closing
does
create
Perpetuum
Mobile
renewable
energy
achieve
sustainability.
Angewandte Chemie International Edition,
Год журнала:
2022,
Номер
61(24)
Опубликована: Март 30, 2022
Polyethylene
terephthalate
(PET)
is
utilized
as
one
of
the
most
popular
consumer
plastics
worldwide,
but
difficulties
associated
with
recycling
PET
have
generated
a
severe
environmental
crisis
ending
its
lifecycle
in
landfills.
We
report
that
zirconium-based
metal-organic
framework
(Zr-MOF)
UiO-66
deconstructs
waste
into
building
blocks
terephthalic
acid
(TA)
and
mono-methyl
(MMT)
within
24
hours
at
260
°C
(total
yield
98
%
under
1
atm
H2
81
Ar).
Extensive
structural
characterization
studies
reveal
during
degradation
process,
undergoes
an
intriguing
transformation
MIL-140A,
which
another
Zr-MOF
shows
good
catalytic
activity
toward
similar
reaction
conditions.
These
results
illustrate
diversity
applications
for
Zr-MOFs
establish
MOFs
new
class
polymer
catalysts
potential
to
address
long-standing
challenges
plastic
waste.
JACS Au,
Год журнала:
2022,
Номер
2(5), С. 1223 - 1231
Опубликована: Май 12, 2022
Interfacial
enzyme
reactions
are
common
in
Nature
and
industrial
settings,
including
the
enzymatic
deconstruction
of
poly(ethylene
terephthalate)
(PET)
waste.
Kinetic
descriptions
PET
hydrolases
necessary
for
both
comparative
analyses,
discussions
structure-function
relations
rational
optimization
technical
processes.
We
investigated
whether
Sabatier
principle
could
be
used
this
purpose.
Specifically,
we
compared
kinetics
two
well-known
hydrolases,
leaf-branch
compost
cutinase
(LCC)
a
from
bacterium
Thermobifida
fusca
(TfC),
when
adding
different
concentrations
surfactant
cetyltrimethylammonium
bromide
(CTAB).
found
that
CTAB
consistently
lowered
strength
enzyme-PET
interactions,
while
its
effect
on
turnover
was
strongly
biphasic.
Thus,
at
gradually
increasing
concentrations,
initially
promoted
subsequently
suppressed.
This
correlation
with
maximal
an
intermediate
binding
accordance
principle.
One
consequence
these
results
enzymes
had
too
strong
intrinsic
interaction
optimal
turnover,
especially
TfC,
which
showed
20-fold
improvement
kcat
maximum.
LCC
other
hand
substrate
affinity
closer
to
optimum,
rate
5-fold
improved
weakened
binding.
Our
may
indeed
rationalize
degradation
support
process
optimization.
Finally,
suggest
future
discovery
efforts
should
consider
because
adsorption
seems
limit
their
catalytic
performance.
Environmental Science & Technology Letters,
Год журнала:
2022,
Номер
9(7), С. 650 - 657
Опубликована: Июнь 17, 2022
The
extensive
production
and
use
of
polyethylene
terephthalate
(PET)
have
generated
an
enormous
amount
plastic
waste,
which
potentially
threatens
the
environment
humans.
Enzyme
biocatalysis
is
a
promising
green
chemistry
alternative,
relative
to
conventional
fossil-derived
process,
achieve
waste
treatment
recycling.
In
this
work,
we
created
biocatalyst,
BIND-PETase,
by
genetically
engineering
curli
Escherichia
coli
cell
with
functional
PETase
enzyme
for
biocatalytic
degradation
PET
plastics.
BIND-PETase
could
degrade
generate
products
at
concentration
level
greater
than
3000
μM
under
various
reaction
conditions.
effects
key
parameters,
including
pH,
temperature,
substrate
mass
load,
surfactant
addition
were
characterized.
was
reusable
remained
stable
no
significant
activity
loss
when
stored
both
4
°C
room
temperature
30
days
(Student's
t
test,
p
>
0.05).
Notably,
enable
microplastics
in
wastewater
effluent
matrix.
Moreover,
depolymerize
highly
crystalline
postconsumer
materials
ambient
conditions
efficiency
9.1%
7
days.
This
study
provides
new
horizon
developing
environmentally
friendly
approaches
solve
recycling
challenge.
