A functional cascading of lignin modification via repression of caffeic acid O-methyltransferase for bioproduction and anti-oxidation in rice

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Crop straws provide substantial biomass resources that are transformable for sustainable biofuels and valuable bioproducts. However, the natural lignocellulose recalcitrance results in an expensive process secondary waste liberation. As lignin is a major recalcitrant factor, genetic engineering of biosynthesis increasingly being implemented bioenergy crops, but much remains unclear about desired alteration resulting function. This study attempted to explore mechanisms modification responsible efficient conversion vitro effective plant anti-oxidation response vivo. We initially selected specific rice mutants by performing modern CRISPR/cas9 editing with caffeic acid O-methyltransferase involved synthetic pathways monolignols (G, S) ferulic (FA), then explored cadmium (Cd) accumulation using advanced chemical, biochemical thermal-chemical analyses. Notable was achieved from predominately synergistic down-regulation S-monomer synthesis three mutants. consequently upgraded porosity up 1.8 folds account significantly enhanced saccharification bioethanol production 20 %-26 % relative wild-type. The modified also favors dissection diverse nanoparticles dimensions reduced 1.5-1.9 folds, applicable into carbon quantum dots increased yields 15 31 %. proportions G-monomers FA were mutants, extractions further assayed higher activities two standard antioxidants (DPPH ABTS) compared wild-type, revealing distinctively antioxidative capacity Water culture showed young mutant seedlings accumulated more Cd than wild-type did (p < 0.01, n = 3), suggesting heavy metal phytoremediation A hypothetical model characteristic reduction, accountable improved recalcitrance, proposed. It provides powerful strategy achieving high-yield value-added bioproducts or enhancing phytoremediation.

Язык: Английский

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Efficient conversion of lignin fractions in lignocellulose using multifunctional polyoxometalate catalysts

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 306, С. 141613 - 141613

Опубликована: Фев. 28, 2025

Язык: Английский

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A multifunctional lignin-based composite ultra-adhesive for wood processing

Green Chemistry, Год журнала: 2023, Номер 25(23), С. 10061 - 10071

Опубликована: Янв. 1, 2023

A high-strength lignin-based composite adhesive has been prepared by the reaction of lignin and furfural in a green DES, excellent flame retardance, thermal insulating, photothermal properties.

Язык: Английский

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Use of lignin-based crude carbon dots as effective antioxidant for natural rubber

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 253, С. 126594 - 126594

Опубликована: Сен. 2, 2023

Язык: Английский

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Unleashing the capacity of Rhodococcus for converting lignin into lipids

Biotechnology Advances, Год журнала: 2023, Номер 70, С. 108274 - 108274

Опубликована: Окт. 31, 2023

Язык: Английский

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