The transformer gene controls sexual development in Drosophila suzukii

Insect Science, Год журнала: 2025, Номер unknown

Опубликована: Март 30, 2025

Abstract The genetic network of sex determination in the model organism Drosophila melanogaster was investigated great detail. Such knowledge not only advances our understanding evolution and regulation sexual dimorphism insects, but also serves as a basis for developing control strategies pest species. In this study, we isolated gene transformer ( Dstra ) from global fruit pest, spotted‐wing suzukii ), characterized its organization. By comparing deduced protein sequence with orthologs 22 species, found that tra genes Dipteran species are divergent. Our research demonstrated undergoes sex‐specific splicing, validated developmental expression profile. We engineered piggyBac‐ based transformation vector expressing complete coding under Tetracycline‐Off system. Through germ‐line transformation, generated 4 independent transgenic lines, producing pseudo‐females chromosomal males absence tetracycline. This observation indicated ectopic , confirmed by detection female transcripts males. exhibited altered wing patterns, feminized abdomen, abnormal reproductive organs, disrupted behavior. Ectopic affected splicing pattern downstream fruitless doublesex . conclusion, study provides comprehensive genetic, morphological, behavioral evidence controls development D. discuss potential applications targeting or using elements.

Язык: Английский

Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae)

Insect Science, Год журнала: 2025, Номер unknown

Опубликована: Май 12, 2025

Abstract Genetic sexing strains (GSS) are important tools for the sterile insect technique (SIT), an environmentally friendly and species‐specific pest control method. GSS feature sex‐specific phenotypes, enabling sex sorting in mass‐rearing facilities male‐only releases, which significantly improve cost‐effectiveness efficiency of SIT programs. In classical GSS, linkage marker gene(s), such as white pupae ( wp ), is achieved through irradiation‐induced translocation between marker‐carrying autosome Y chromosome. However, this approach may render males semisterile. The recently proposed neo‐classical concept suggests using genome editing to achieve by directly inserting wild‐type allele onto chromosome, potentially yielding with higher fertility. study, we examined Ceratitis capitata gene a genetic developed minimal, intronless version gene, termed mini‐ . We demonstrate that single copy sufficient restore brown puparium phenotype functional when integrated at various positions within C. genome, including X Due its smaller size (4689 bp, 2000 bp putative promoter region) relative full (20868 bp), facilitate precise insertion into representing step toward realizing GSS. Furthermore, methodology designing testing medfly be adapted other Tephritid species identified gene.

Язык: Английский

Insect Pest Control from Chemical to Biotechnological Approach: Constrains and Challenges

Insects, Год журнала: 2025, Номер 16(5), С. 528 - 528

Опубликована: Май 15, 2025

The large growth in the global population requires new solutions for control of harmful insects that compete our food. Changing regulatory requirements and public perception, together with continuous evolution resistance to conventional insecticides, also require, addition innovative molecules different modes action, non-chemical strategies can help maintain efficient integrated pest management programs. last 30 years have inaugurated a era characterised by discovery mechanisms action chemical families. Although European programs promote green deal crop protection sector, existing thorough regulations slow down its spread adoption products. In light these changes, this review will describe more detail dynamics registration insecticides difficulties agrochemical industries encounter. Subsequently, alternative based on natural substances origin, entomopathogenic microorganisms, semiochemical semiophysical compounds, classical augmentative biological be described. advantages illustrated constrains their diffusion commercialisation. Finally, main biotechnological discoveries described, from transgenic plants symbiotic control, genetic and, recently, insect genomic transformation or RNAi. These biotechnologies revolutionise sector despite some related restrictions present countries.

Язык: Английский