Regenerative medicine and dentistry., Год журнала: 2025, Номер unknown, С. 5 - 5
Опубликована: Март 21, 2025
Perspective hiPSC-Driven Organoid Construction and Application Prospects Bangheng Liu 1,2, Yulei Mu 2,3 Dong-An Wang 1,2,* 1 Department of Biomedical Engineering, Chinese University Hong Kong, Sha Tin, New Territories, Kong SAR 999077, China 2 Center for Neuromusculoskeletal Restorative Medicine, InnoHK, HKSTP, 3 City 83 Tat Chee Avenue, Kowloon, * Correspondence: [email protected] Received: 5 March 2025; Revised: 19 Accepted: 20 Published: 21 2025 Abstract: Induced pluripotent stem cell (iPSC)-derived organoid platforms can simulate various target tissues hold broad application prospects in personalized medicine, disease modeling, drug screening, organ transplantation, understanding development mechanisms. Currently, the human iPSC (hiPSC) organoids is gradually shifting towards Matrigel-free scaffold-free systems, promoting precise control over composition structure these systems establishing induction protocols specialized organoids. Researchers are also exploring construction multifunctional with complex structures material exchange channels through vascularization, segmented induction, assembly technologies, though further breakthroughs needed. In future, hiPSC expected to advance precision treatment, high-throughput module detection multi-organ integration, automation. Additionally, when combined large artificial intelligence models, there potential establish data medical platforms, providing support clinical decision-making. Moreover, AI anticipated foster collaboration rather than competition, coordinated growth field. For hiPSC-derived it crucial enhance ethical review framework balance radical scientific exploration conservative public attitudes. must optimize or develop new reduce genomic instability tumorigenic risks, while avoiding emergence non-target cells insufficient functional maturity.
Язык: Английский
Процитировано
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Frontiers in Pharmacology, Год журнала: 2025, Номер 16
Опубликована: Март 25, 2025
The persistently high mortality rates associated with cancer underscore the imperative need for innovative, efficacious, and safer therapeutic agents, as well a more nuanced understanding of tumor biology. Patient-derived organoids (PDOs) have emerged innovative preclinical models significant translational potential, capable accurately recapitulating structural, functional, heterogeneous characteristics primary tumors. When integrated cutting-edge genomic tools such CRISPR, PDOs provide powerful platform identifying driver genes novel targets. This comprehensive review delves into recent advancements in CRISPR-mediated functional screens leveraging across diverse types, highlighting their pivotal role high-throughput genomics microenvironment (TME) modeling. Furthermore, this highlights synergistic potential integrating CRISPR immunotherapy, focusing on uncovering immune evasion mechanisms improving efficacy immunotherapeutic approaches. Together, these technologies offer promise advancing precision oncology.
Язык: Английский
Процитировано
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Current Opinion in Neurobiology, Год журнала: 2025, Номер 92, С. 103018 - 103018
Опубликована: Апрель 11, 2025
The field of brain organoids has experienced a period rapid and transformative growth, enabling researchers to investigate complex human biological mechanisms that were previously deemed intractable. This review provides an overview the current landscape organoids, with particular focus on their relevance in context neurodevelopmental disorders. It also emphasizes crucial role these models play elucidating both cell-autonomous non-cell-autonomous mechanisms. We describe how two mechanisms, often considered be independent, are intricately interconnected. In conclusion, this aims highlight utilization considerably advanced our comprehension disorders, while delineating prospective avenues for investigating conditions.
Язык: Английский
Процитировано
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Опубликована: Апрель 15, 2025
Язык: Английский
Процитировано
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