Fluorescent/colorimetric dual-mode for detecting of MC-LR using bidirectional RCA coupled with CdTe QDs
Microchimica Acta,
Год журнала:
2025,
Номер
192(3)
Опубликована: Фев. 25, 2025
Язык: Английский
Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors
Biosensors,
Год журнала:
2025,
Номер
15(3), С. 155 - 155
Опубликована: Март 2, 2025
High-sensitivity
and
high-specificity
biodetection
is
critical
for
advancing
applications
in
life
sciences,
biosafety,
food
safety,
environmental
monitoring.
CRISPR/Cas
systems
have
emerged
as
transformative
tools
biosensing
due
to
their
unparalleled
specificity,
programmability,
unique
enzymatic
activities.
They
exhibit
two
key
cleavage
behaviors:
precise
ON-target
guided
by
specific
protospacers,
which
ensures
accurate
target
recognition,
bystander
activity
triggered
upon
binding,
enables
robust
signal
amplification.
These
properties
make
highly
versatile
designing
biosensors
ultra-sensitive
detection.
This
review
comprehensively
explores
recent
advancements
system-based
biosensors,
highlighting
impact
on
improving
performance.
We
discuss
the
integration
of
with
diverse
readout
mechanisms,
including
electrochemical,
fluorescent,
colorimetric,
surface-enhanced
Raman
scattering
(SERS),
so
on.
Additionally,
we
examine
development
integrated
systems,
such
microfluidic
devices
portable
leverage
technology
point-of-care
testing
(POCT)
high-throughput
analysis.
Furthermore,
identify
unresolved
challenges,
aiming
inspire
innovative
solutions
accelerate
translation
these
technologies
into
practical
diagnostics,
food,
environment
safety.
Язык: Английский
Precise pathogen quantification by CRISPR-Cas: a sweet but tough nut to crack
Critical Reviews in Microbiology,
Год журнала:
2024,
Номер
unknown, С. 1 - 19
Опубликована: Сен. 17, 2024
Pathogen
detection
is
increasingly
applied
in
medical
diagnosis,
food
processing
and
safety,
environmental
monitoring.
Rapid,
sensitive,
accurate
pathogen
quantification
the
most
critical
prerequisite
for
assessing
protocols
preventing
risks.
Among
various
methods
evolved,
those
based
on
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-associated
proteins
(Cas)
have
been
developed
as
important
strategies
due
to
their
distinct
advantages
of
rapid
target
recognition,
programmability,
ultra-specificity,
potential
scalability
point-of-care
testing
(POCT).
However,
arguments
concerns
quantitative
capability
CRISPR-based
are
ongoing.
Herein,
we
systematically
overview
according
principles,
properties,
application
scenarios.
Notably,
review
future
challenges
perspectives
address
precise
by
CRISPR-Cas.
We
hope
insights
presented
this
will
benefit
development
methods.
Язык: Английский
Microfluidics for foodborne bacteria analysis: Moving toward multiple technologies integration
Biomicrofluidics,
Год журнала:
2024,
Номер
18(6)
Опубликована: Ноя. 4, 2024
Food
security
related
to
bacterial
pathogens
has
seriously
threatened
human
life
and
caused
public
health
problems.
Most
of
the
reported
methods
are
targeted
at
known
major
commonly
found
in
food
samples,
but
some
extent,
they
have
disadvantage
lacking
simplicity,
speed,
high
throughput,
sensitivity.
Microfluidics
become
a
promising
tool
for
foodborne
bacteria
analysis
addresses
above
limitations.
In
this
perspective,
we
briefly
discussed
ongoing
research
development
field.
We
outline
types
microfluidics,
strategies
target
biorecognition,
signal
amplification
technologies
microfluidic
system
analysis.
also
proposed
future
directions
microfluidics
analysis,
which
aims
integrate
multiple
toward
intelligent
with
selectivity
sensitivity
unknown
detection,
simultaneous
detection
pollutants.
Язык: Английский