Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 27, 2024
Introduction
This
study
is
the
final
part
of
a
two-part
series
that
delves
into
molecular
mechanisms
driving
adaptive
laboratory
evolution
(ALE)
Salmonella
enterica
in
acid
stress.
The
phenotypic
and
transcriptomic
alterations
acid-evolved
lineages
(EL)
serovar
Enteritidis
after
70
days
stress
exposure
were
analyzed.
Materials
methods
stability
changes
observed
acetic
was
explored
removal
using
newly
developed
evolutionary
lineage
EL5.
Additionally,
impact
short-term
on
previously
adapted
EL4
also
examined.
Results
results
indicate
elevated
antibiotic
minimum
inhibitory
concentration
(MIC)
to
for
lost
when
removed.
phenomenon
against
human
antibiotics
such
as
meropenem,
ciprofloxacin,
gentamicin,
streptomycin.
MIC
meropenem
day
0.094
mM,
which
dropped
0.032
mM
removed
from
70.
However,
reintroduction,
swiftly
elevated,
within
4
days,
it
returned
mM.
After
20
more
adaptation
acid,
increased
0.125
other
tested
exhibited
similar
trend.
be
35
remained
constant
even
Readaptation
caused
increase
37
Bacterial
whole
genome
sequencing
EL5
revealed
base
substitutions
several
genes
involved
pathogenesis,
phoQ
wzc
genes.
Transcriptomic
analysis
upregulation
virulence,
drug
resistance,
toxin-antitoxin,
iron
metabolism
Unstable
S
almonella
small
colony
variants
(SSCV)
.
compared
wild-type
unevolved
Enteritidis.
Discussion
presents
comprehensive
understanding
phenotypic,
genomic,
due
prolonged
through
ALE.
ACS Infectious Diseases,
Год журнала:
2024,
Номер
10(8), С. 2795 - 2813
Опубликована: Июль 30, 2024
The
potential
antimicrobial
activity
and
low
propensity
to
induce
the
development
of
bacterial
resistance
have
rendered
peptides
(AMPs)
as
novel
ideal
candidate
therapeutic
agents
for
treatment
infections
caused
by
drug-resistant
pathogenic
bacteria.
targeting
membranes
AMPs
has
been
typically
considered
their
sole
mode
action;
however,
increasing
evidence
supports
existence
multiple
complementary
functions
that
result
in
death.
An
in-depth
characterization
mechanism
action
could
facilitate
further
research
with
higher
potency.
current
study
employs
biophysics
proteomics
approaches
unveil
mechanisms
underlying
antibacterial
A11,
a
AMP,
against
Abstract
Dissimilatory
nitrate
reduction
to
ammonium
(DNRA)
has
garnered
attention
due
its
ability
recover
ammonia
and
reduce
greenhouse
gas
emissions
simultaneously.
In
this
study,
the
potential
of
using
static
magnetic
field
(SMF)
improve
DNRA
process
was
explored
from
sight
molecular
biology.
Functional
genes,
microbial
community
structure,
metabolism
pathways
were
discussed.
SMF
40
mT
shortened
start-up
time
75
days
41
days,
while
80
delayed
it
103
days.
On
day
80,
rate
under
SMF,
reached
174
±
11
μmol
kg
−1
h
,
significantly
surpassing
0
(88
6
−
1
)
(52
4
).
also
accelerated
succession
enrichment
functional
bacteria
like
Geobacter
(from
15.71%
32.11%).
qPCR
results
suggested
that
promoted
rapid
gene
nrfA
nirS
on
40.
Dynamic
responses
Thauera
sp.
RT1901,
Stutzerimonas
stutzeri
Shewanella
oneidensis
MR-1
loihica
PV-4
at
transcriptional
levels
confirmed
could
nitrogen
removal
electron
transfer
denitrification
bacteria.
Consequently,
work
validated
possibility
for
recovery
investigated
underlying
mechanisms,
which
promote
application
in
full-scale.
Foods,
Год журнала:
2024,
Номер
13(16), С. 2495 - 2495
Опубликована: Авг. 8, 2024
Lactic
acid
bacteria
(LAB)
are
the
most
common
probiotics,
and
they
present
excellent
inhibitory
effects
on
pathogenic
bacteria.
This
study
aimed
to
explore
anti-biofilm
potential
of
purified
active
substance
Lactiplantibacillus
plantarum,
named
Z102-E.
The
Z102-E
Listeria
monocytogenes
were
investigated
in
detail,
a
transcriptomic
analysis
was
conducted
reveal
mechanism.
results
indicated
that
sub-MIC
(3.2,
1.6,
0.8
mg/mL)
decreased
bacterial
growth
effectively
reduced
self-aggregation,
surface
hydrophobicity,
sugar
utilization,
motility,
biofilm
formation,
AI-2
signal
molecule,
contents
extracellular
polysaccharides,
protein
L.
monocytogenes.
Moreover,
inverted
fluorescence
microscopy
observation
confirmed
effect
117
genes
up-regulated
214
down-regulated.
regulated
expressions
related
quorum
sensing,
etc.
These
findings
suggested
has
great
application
as
natural
bacteriostatic
agent.
Foods,
Год журнала:
2024,
Номер
13(17), С. 2808 - 2808
Опубликована: Сен. 4, 2024
Cronobacter
sakazakii
(C.
sakazakii)
is
a
foodborne
pathogen
capable
of
causing
severe
infections
in
newborns.
The
PhoP/PhoQ
two-component
system
exerts
significant
influence
on
bacterial
virulence.
This
study
aimed
to
investigate
the
impact
intestinal
inflammation
neonatal
mice
induced
by
C.
sakazakii.
Neonatal
were
infected
orally
BAA-894
(WT),
phoPQ-gene-deletion
strain
(ΔphoPQ),
and
complementation
(ΔphoPQC),
was
monitored.
Deletion
phoPQ
gene
reduced
viable
count
ileum
alleviated
tissue
damage.
Moreover,
caspase-3
activity
WT-
ΔphoPQC-infected
significantly
elevated
compared
that
ΔphoPQ
control
groups.
ELISA
results
showed
levels
TNF-α
IL-6
with
WT
ΔphoPQC.
In
addition,
deletion
resulted
down-regulation
inflammatory
genes
(IL-1β,
TNF-α,
IL-6,
NF-κB
p65,
TLR4)
within
decreased
modulating
TLR4/NF-κB
pathway.
It
suggested
targeting
could
be
potential
strategy
for
mitigating
sakazakii-induced
infections.
