Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 27, 2024
Introduction
This
study
is
the
final
part
of
a
two-part
series
that
delves
into
molecular
mechanisms
driving
adaptive
laboratory
evolution
(ALE)
Salmonella
enterica
in
acid
stress.
The
phenotypic
and
transcriptomic
alterations
acid-evolved
lineages
(EL)
serovar
Enteritidis
after
70
days
stress
exposure
were
analyzed.
Materials
methods
stability
changes
observed
acetic
was
explored
removal
using
newly
developed
evolutionary
lineage
EL5.
Additionally,
impact
short-term
on
previously
adapted
EL4
also
examined.
Results
results
indicate
elevated
antibiotic
minimum
inhibitory
concentration
(MIC)
to
for
lost
when
removed.
phenomenon
against
human
antibiotics
such
as
meropenem,
ciprofloxacin,
gentamicin,
streptomycin.
MIC
meropenem
day
0.094
mM,
which
dropped
0.032
mM
removed
from
70.
However,
reintroduction,
swiftly
elevated,
within
4
days,
it
returned
mM.
After
20
more
adaptation
acid,
increased
0.125
other
tested
exhibited
similar
trend.
be
35
remained
constant
even
Readaptation
caused
increase
37
Bacterial
whole
genome
sequencing
EL5
revealed
base
substitutions
several
genes
involved
pathogenesis,
phoQ
wzc
genes.
Transcriptomic
analysis
upregulation
virulence,
drug
resistance,
toxin-antitoxin,
iron
metabolism
Unstable
S
almonella
small
colony
variants
(SSCV)
.
compared
wild-type
unevolved
Enteritidis.
Discussion
presents
comprehensive
understanding
phenotypic,
genomic,
due
prolonged
through
ALE.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(23), С. 16679 - 16679
Опубликована: Ноя. 23, 2023
The
rhizosphere
microbiota,
which
includes
plant
growth-promoting
rhizobacteria
(PGPR),
is
essential
for
nutrient
acquisition,
protection
against
pathogens,
and
abiotic
stress
tolerance
in
plants.
However,
agricultural
practices
affect
the
composition
functions
of
reducing
their
beneficial
effects
on
growth
health.
Among
PGPR,
rhizobia
form
mutually
symbiosis
with
legumes.
In
this
study,
we
characterized
16
clover
nodule
isolates
from
non-farmed
soil
to
explore
(PGP)
potential,
hypothesizing
that
these
bacteria
may
possess
unique,
unaltered
PGP
traits,
compared
those
affected
by
common
practices.
Biolog
profiling
revealed
versatile
metabolic
capabilities,
enabling
them
utilize
a
wide
range
carbon
energy
sources.
All
were
effective
phosphate
solubilizers,
individual
strains
exhibited
1-aminocyclopropane-1-carboxylate
deaminase
metal
ion
chelation
activities.
Metabolically
active
showed
improved
performance
symbiotic
interactions
Comparative
genomics
genomes
five
contained
significantly
enriched
fraction
unique
genes
associated
quorum
sensing
aromatic
compound
degradation.
As
potential
PGPR
agriculture
grows,
emphasize
importance
molecular
characterization
traits
as
fundamental
step
towards
subsequent
application
field
an
alternative
chemical
fertilizers
supplements.
Advances in Drug and Alcohol Research,
Год журнала:
2023,
Номер
3
Опубликована: Дек. 8, 2023
Bioactive
lipids
such
as
endocannabinoids
serve
important
modulators
of
host
health
and
disease
through
their
effects
on
various
functions
including
central
metabolism,
gut
physiology,
immunity.
Furthermore,
changes
to
the
microbiome
caused
by
external
factors
diet
or
development
have
been
associated
with
altered
endocannabinoid
tone
outcomes.
These
observations
suggest
existence
reciprocal
relationships
between
lipid
signaling
networks
bacterial
populations
that
reside
within
gut.
Indeed,
congeners
N-
acylethanolamides
recently
shown
alter
growth,
functions,
behaviors,
therefore
introducing
putative
mechanisms
which
these
bioactive
directly
modulate
microbiome.
Moreover,
potential
interactions
add
another
layer
complexity
regulation
pathogenesis
may
be
mediated
derivatives.
This
mini
review
will
summarize
recent
literature
exemplifies
how
monoacylglycerols
can
impact
in
vitro
We
also
highlight
exciting
preclinical
studies
engineered
bacteria
synthesize
precursors
strategies
treat
diseases
are
part
driven
aberrant
signaling,
obesity
inflammatory
bowel
diseases.
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 27, 2024
Introduction
This
study
is
the
final
part
of
a
two-part
series
that
delves
into
molecular
mechanisms
driving
adaptive
laboratory
evolution
(ALE)
Salmonella
enterica
in
acid
stress.
The
phenotypic
and
transcriptomic
alterations
acid-evolved
lineages
(EL)
serovar
Enteritidis
after
70
days
stress
exposure
were
analyzed.
Materials
methods
stability
changes
observed
acetic
was
explored
removal
using
newly
developed
evolutionary
lineage
EL5.
Additionally,
impact
short-term
on
previously
adapted
EL4
also
examined.
Results
results
indicate
elevated
antibiotic
minimum
inhibitory
concentration
(MIC)
to
for
lost
when
removed.
phenomenon
against
human
antibiotics
such
as
meropenem,
ciprofloxacin,
gentamicin,
streptomycin.
MIC
meropenem
day
0.094
mM,
which
dropped
0.032
mM
removed
from
70.
However,
reintroduction,
swiftly
elevated,
within
4
days,
it
returned
mM.
After
20
more
adaptation
acid,
increased
0.125
other
tested
exhibited
similar
trend.
be
35
remained
constant
even
Readaptation
caused
increase
37
Bacterial
whole
genome
sequencing
EL5
revealed
base
substitutions
several
genes
involved
pathogenesis,
phoQ
wzc
genes.
Transcriptomic
analysis
upregulation
virulence,
drug
resistance,
toxin-antitoxin,
iron
metabolism
Unstable
S
almonella
small
colony
variants
(SSCV)
.
compared
wild-type
unevolved
Enteritidis.
Discussion
presents
comprehensive
understanding
phenotypic,
genomic,
due
prolonged
through
ALE.
