Antibodies to the RBD of SARS-CoV-2 spike mediate productive infection of primary human macrophages DOI Creative Commons
Suzanne Pickering, Harry Wilson, Elena Bravo

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 30, 2024

The role of myeloid cells in the pathogenesis SARS-CoV-2 is well established, particular as drivers cytokine production and systemic inflammation characteristic severe COVID-19. However, potential for to act bona fide targets productive infection, specifics entry, remain unclear. Using a panel anti-SARS-CoV-2 monoclonal antibodies (mAbs) we performed detailed assessment antibody-mediated infection monocytes/macrophages. mAbs with most consistent mediate were those targeting conserved region receptor binding domain (RBD; group 1/class 4). Infection was closely related neutralising concentration mAbs, peak occurring below IC50, while pre-treating remdesivir or FcγRI-blocking inhibited infection. Studies primary macrophages demonstrated high-level infected appearing multinucleated syncytial. not seen absence antibody same quantity virus. Addition ruxolitinib significantly increased indicating restraint through innate immune mechanisms rather than entry. High-level pro-inflammatory cytokines directly correlated macrophage levels. We hypothesise that via antibody-FcR interactions could contribute virus spread persistent

Язык: Английский

Longitudinal humoral immunity against SARS-CoV-2 Spike following infection in individuals from Cameroon DOI
Mehdi Benlarbi,

Dell-Dylan Kenfack,

Katrina Dionne

и другие.

Virology, Год журнала: 2025, Номер 605, С. 110467 - 110467

Опубликована: Фев. 25, 2025

Язык: Английский

Процитировано

1
mRNA vaccines with RBD mutations have broad-spectrum activity against SARS-CoV-2 variants in mice DOI Creative Commons
Xiaoming Liang,

Yuxia Yuan,

Junbin Wang

и другие.

npj Vaccines, Год журнала: 2025, Номер 10(1)

Опубликована: Янв. 13, 2025

The emergence of SARS-CoV-2 variants with defined mutations that enhance pathogenicity or facilitate immune evasion has resulted in a continual decline the protective efficacy existing vaccines. Therefore, there is pressing need for vaccine capable combating future variants. In this study, we designed new mRNA vaccines, BSCoV05 and BSCoV06, generated point receptor-binding domain (RBD) original Wuhan strain to increase their broad-spectrum antiviral activity. Additionally, used BA.1 RBD as control. Both vaccines elicited robust response BALB/c K18-hACE2 mice, generating high levels specific binding antibodies against BA.2 RBD. Moreover, all three induced neutralizing prototype viral relevant variants, including Alpha Beta strains Omicron BA.1, BA.2, BA.5, XBB.1.5, XBB.1.16, EG.5.1, EG.5.1.1, BSCoV06 demonstrating broader antibody also cellular response. After challenge, both BSCOV06 provided protection EG.5.1 mouse strains. these two merit further evaluation nonhuman primates, design strategy should be explored its potential application offering valuable insights into development.

Язык: Английский

Процитировано

0
Recurrent waning of anti-SARS-CoV-2 neutralizing antibodies despite multiple antigen encounters DOI Creative Commons
Edwards Pradenas, Víctor Urrea, Sílvia Marfil

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Abstract Background: SARS-CoV-2 neutralizing antibodies may protect against symptomatic infection in immunized individuals. However, vaccine-induced antibody levels wane over time, reducing vaccine efficacy. The definition of the waning kinetics SARS-CoV-2 responses and potential impact sequential antigen encounters are still poorly defined. Methods: Plasma activity was determined longitudinally collected samples from infected, primo-vaccinated boosted Neutralizing decay were modeled time using Log-Log biexponential models. Results:Neutralizing after an initial peak all groups vaccinated individuals with half-life ranging 29 to 60 days. Exponential models showed a subsequent stabilization titers plateau. Both response plateau values depended on type, status severity infection. Booster immunization by either vaccines or breakthrough infections did not modify peak, rate values. Conclusions:Our results indicate that recurrent even several encounters. Repeated immunizations would be required maintain high vulnerable

Язык: Английский

Процитировано

0
Mucosal adenovirus vaccine Ad5-XBB.1.5 boosting elicits nasal IgA and transiently prevents JN.1 wave infection for less than 6 months in real-world settings DOI Creative Commons

Yanqun Wang,

Peilan Wei,

Jun Zhang

и другие.

hLife, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Kinetics of naturally induced binding and neutralising anti-SARS-CoV-2 antibody levels and potencies among SARS-CoV-2 infected Kenyans with diverse grades of COVID-19 severity: an observational study DOI Creative Commons
John Kimotho, Yiakon Sein, Shahin Sayed

и другие.

Wellcome Open Research, Год журнала: 2024, Номер 8, С. 350 - 350

Опубликована: Дек. 2, 2024

Background Given the low levels of coronavirus disease 2019 (COVID-19) vaccine coverage in sub-Saharan Africa (sSA), despite high natural severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) exposures, strategies for extending breadth and longevity naturally acquired immunity are warranted. Designing such will require a good understanding immunity. Methods We measured whole-spike immunoglobulin G (IgG) spike-receptor binding domain (RBD) total immunoglobulins (Igs) on 585 plasma samples collected longitudinally over five successive time points within six months COVID-19 diagnosis 309 patients. antibody-neutralising potency against wild-type (Wuhan) SARS-CoV-2 pseudovirus subset 51 patients three points. Binding neutralising antibody potencies were then tested correlations with severities. Results Rates seroconversion increased from day 0 (day PCR testing) to 180 (six months) (63.6% 100 %) (69.3 % 97%) anti-spike-IgG anti-spike-RBD Igs, respectively. Levels these antibodies peaked at 28 (p<0.01) subsequently maintained without significant decay (p>0.99). Similarly, but declined by three-fold, after (p<0.01). highly correlated all analysed (r>0.60, p<0.01). severity. Conclusions Most generated specific that remained stable first infection. However, respective decayed three-fold month-six suggesting they short-lived, consistent what has been observed elsewhere world. Thus, regular vaccination boosters required sustain anti-SARS-CoV-2 our population.

Язык: Английский

Процитировано

0
Coronaviruses: Pandemic SARS-CoV-2 DOI
Benjamin J. Cowling, Sook‐San Wong

Springer eBooks, Год журнала: 2024, Номер unknown, С. 1 - 79

Опубликована: Дек. 18, 2024

Язык: Английский

Процитировано

0
Antibodies to the RBD of SARS-CoV-2 spike mediate productive infection of primary human macrophages DOI Creative Commons
Suzanne Pickering, Harry Wilson, Elena Bravo

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 30, 2024

The role of myeloid cells in the pathogenesis SARS-CoV-2 is well established, particular as drivers cytokine production and systemic inflammation characteristic severe COVID-19. However, potential for to act bona fide targets productive infection, specifics entry, remain unclear. Using a panel anti-SARS-CoV-2 monoclonal antibodies (mAbs) we performed detailed assessment antibody-mediated infection monocytes/macrophages. mAbs with most consistent mediate were those targeting conserved region receptor binding domain (RBD; group 1/class 4). Infection was closely related neutralising concentration mAbs, peak occurring below IC50, while pre-treating remdesivir or FcγRI-blocking inhibited infection. Studies primary macrophages demonstrated high-level infected appearing multinucleated syncytial. not seen absence antibody same quantity virus. Addition ruxolitinib significantly increased indicating restraint through innate immune mechanisms rather than entry. High-level pro-inflammatory cytokines directly correlated macrophage levels. We hypothesise that via antibody-FcR interactions could contribute virus spread persistent

Язык: Английский

Процитировано

0