Soil-Gradient-Derived Bacterial Synthetic Communities Enhance Drought Tolerance in Quercus pubescens and Sorbus domestica Seedlings DOI Creative Commons
Ivan Aleksieienko, Mariana Fernandes Hertel,

Jérôme Reilhan

и другие.

Plants, Год журнала: 2025, Номер 14(11), С. 1659 - 1659

Опубликована: Май 29, 2025

Climate-change-induced drought threatens forest restoration by limiting seedling establishment. To address this, we developed synthetic bacterial communities (SynComs) tailored to support tolerance in two Mediterranean tree species, Quercus pubescens and Sorbus domestica. Bacteria were isolated from soil exposed long-term drought, sampling across depths root-associated compartments. We selected strains with key plant-beneficial traits, including exopolysaccharide (EPS) production, hormone synthesis (auxin, ABA), siderophore release, osmotic tolerance. SynComs assembled based on functional complementarity ecological origin. Biofilm assays showed that even weak individual producers could enhance community-level performance. After initial screening Arabidopsis thaliana, the most least effective tested Q. S. domestica seedlings. Compared controls, best-performing reduced proportion of drought-symptomatic seedlings 47% 71% domestica, outperforming single-strain inoculants. Notably, EPS-rich SynCom B aligned conservative root traits pubescens, while hormone-rich F matched acquisitive strategy Predictive modeling identified identity symptom timing as predictors resilience. Our results highlight value matching microbial plant strategies context for climate-smart restoration.

Язык: Английский

Targeted manipulation of food webs in the plant rhizosphere DOI
Lu Luan, Francisco Dini‐Andreote, Shungui Zhou

и другие.

Trends in Plant Science, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Microbiome Migration from Soil to Leaves in Maize and Rice DOI Creative Commons

Jiejia Ma,

Qianze Peng,

Silu Chen

и другие.

Microorganisms, Год журнала: 2025, Номер 13(4), С. 947 - 947

Опубликована: Апрель 20, 2025

The interactions between plants and microbes are essential for enhancing crop productivity. However, the mechanisms underlying host-specific microbiome migration functional assembly remain poorly understood. In this study, from soil to leaves in rice (Oryza sativa) maize (Zea mays) was analyzed through 16S rRNA sequencing phenotypic assessments. When we used same source grow maize, microbiota traits were specifically enriched by its phyllosphere rhizosphere. This indicated that can selectively assemble microbiomes a shared source. Therefore, 22 strains isolated phyllospheres of construct synthetic microbial community (SynCom). growth inoculated with SynCom, belonging Bacillus compared phyllosphere. Additionally, strain Rhizobium rhizosphere These results suggest plant species influence within their respective compartments. Compared mock inoculation, SynCom inoculation significantly enhanced growth. microbiomes, Achromobacter, which assembled both played role Our findings underscore importance dynamics leveraging plant–microbe sustainable agriculture.

Язык: Английский

Процитировано

0
Assembly and application of a synthetic bacterial community for enhancing barley tolerance to drought DOI Creative Commons

Linda Rigerte,

Anna Heintz‐Buschart, Thomas Reitz

и другие.

Frontiers in Bacteriology, Год журнала: 2025, Номер 4

Опубликована: Апрель 25, 2025

Under climate change scenarios for temperate regions in Europe, prolonged droughts pose a major threat to barley production, but few studies have been conducted on stress mitigation strategies using plant-beneficial rhizobacteria. With this mind, we isolated and screened culture collection of drought-tolerant bacteria from the rhizosphere. From collection, assembled 16-member consortium based their relative abundances rhizosphere after drought vitro osmotic tolerance (Drought Tolerant Synthetic microbial Community/”DT-SynCom”). Members DT-SynCom range Proteobacteria Firmicutes Actinobacteria. We used Oxford Nanopore Illumina technologies assemble complete genomes. Whole genome annotation revealed presence number genes associated with plant growth promoting traits such as IAA biosynthesis, ACC deaminase activity siderophore production. In assays confirmed auxin activity, inorganic P solubilization, cellulase chitinase activities by selected bacterial strains. The members were not antagonistic each other, either neutral or beneficial shoot root when applied individually . To clarify effect designed tolerance, pot experiment was under conditions. reduced wilting leaves had positive drought. results research suggest that result improved stress.

Язык: Английский

Процитировано

0
Rhizosphere bacterial community confers drought tolerance to Astragalus mongholicus DOI Creative Commons
Shuyan Li, Pei‐Rong Li, Hao Ding

и другие.

Agricultural Water Management, Год журнала: 2025, Номер 315, С. 109543 - 109543

Опубликована: Май 10, 2025

Язык: Английский

Процитировано

0
Soil-Gradient-Derived Bacterial Synthetic Communities Enhance Drought Tolerance in Quercus pubescens and Sorbus domestica Seedlings DOI Creative Commons
Ivan Aleksieienko, Mariana Fernandes Hertel,

Jérôme Reilhan

и другие.

Plants, Год журнала: 2025, Номер 14(11), С. 1659 - 1659

Опубликована: Май 29, 2025

Climate-change-induced drought threatens forest restoration by limiting seedling establishment. To address this, we developed synthetic bacterial communities (SynComs) tailored to support tolerance in two Mediterranean tree species, Quercus pubescens and Sorbus domestica. Bacteria were isolated from soil exposed long-term drought, sampling across depths root-associated compartments. We selected strains with key plant-beneficial traits, including exopolysaccharide (EPS) production, hormone synthesis (auxin, ABA), siderophore release, osmotic tolerance. SynComs assembled based on functional complementarity ecological origin. Biofilm assays showed that even weak individual producers could enhance community-level performance. After initial screening Arabidopsis thaliana, the most least effective tested Q. S. domestica seedlings. Compared controls, best-performing reduced proportion of drought-symptomatic seedlings 47% 71% domestica, outperforming single-strain inoculants. Notably, EPS-rich SynCom B aligned conservative root traits pubescens, while hormone-rich F matched acquisitive strategy Predictive modeling identified identity symptom timing as predictors resilience. Our results highlight value matching microbial plant strategies context for climate-smart restoration.

Язык: Английский

Процитировано

0