Human Genetics,
Год журнала:
2016,
Номер
136(1), С. 1 - 12
Опубликована: Ноя. 2, 2016
The
next-generation
sequencing
identifies
a
growing
number
of
candidate
genes
associated
with
human
genetic
diseases,
which
inevitably
requires
efficient
methods
to
validate
the
causal
links
between
genotype
and
phenotype.
Recently,
zebrafish,
sufficiently
high-throughput
capabilities,
has
become
favored
option
study
pathogenesis.
In
addition,
CRISPR/Cas9-based
approaches
have
radically
reduced
efforts
introduce
targeted
genome
engineering
in
various
organisms.
Here,
we
systemically
review
basic
considerations
design
gene
editing
zebrafish
CRISPR/Cas9,
explore
potential
combination
these
two
support
functional
analysis
variants.
Chemical Research in Toxicology,
Год журнала:
2019,
Номер
33(1), С. 95 - 118
Опубликована: Окт. 18, 2019
Unpredicted
human
safety
events
in
clinical
trials
for
new
drugs
are
costly
terms
of
health
and
money.
The
drug
discovery
industry
attempts
to
minimize
those
with
diligent
preclinical
testing.
Current
standard
practices
good
at
preventing
toxic
compounds
from
being
tested
the
clinic;
however,
false
negative
toxicity
results
still
a
reality.
Continual
improvement
must
be
pursued
realm.
Higher-quality
therapies
can
brought
forward
more
information
about
potential
toxicities
associated
mechanisms.
zebrafish
model
is
bridge
between
vitro
assays
mammalian
vivo
studies.
This
powerful
its
breadth
application
tractability
research.
In
past
two
decades,
our
understanding
disease
biology
has
grown
significantly
owing
thousands
studies
on
this
tiny
vertebrate.
Review
summarizes
challenges
strengths
model,
discusses
3Rs
value
that
it
deliver,
highlights
translatable
untranslatable
biology,
brings
together
reports
recent
focusing
toxicology.
Biomedicines,
Год журнала:
2019,
Номер
7(1), С. 23 - 23
Опубликована: Март 26, 2019
Zebrafish
larvae
show
a
clear
and
distinct
pattern
of
swimming
in
response
to
light
dark
conditions,
following
the
development
swim
bladder
at
4
days
post
fertilization.
This
behavior
is
increasingly
employed
screening
neuroactive
drugs.
The
recent
emergence
high-throughput
techniques
for
automatic
tracking
zebrafish
has
further
allowed
an
objective
efficient
way
finding
subtle
behavioral
changes
that
could
go
unnoticed
during
manual
observations.
review
highlights
use
as
model
compounds.
We
describe,
brief,
repertoire
larvae.
Then,
we
focus
on
utilization
light-dark
locomotion
test
identifying
Zebrafish,
Год журнала:
2017,
Номер
14(3), С. 197 - 208
Опубликована: Май 1, 2017
Modeling
of
stress
and
anxiety
in
adult
zebrafish
(Danio
rerio)
is
increasingly
utilized
neuroscience
research
central
nervous
system
(CNS)
drug
discovery.
Representing
the
most
commonly
used
models,
novel
tank
test
(NTT)
focuses
on
diving
response
to
potentially
threatening
stimuli,
whereas
light-dark
(LDT)
based
fish
scototaxis
(innate
preference
for
dark
vs.
bright
areas).
Here,
we
systematically
evaluate
utility
these
two
tests,
combining
meta-analyses
published
literature
with
comparative
vivo
behavioral
whole-body
endocrine
(cortisol)
testing.
Overall,
NTT
LDT
behaviors
demonstrate
a
generally
good
cross-test
correlation
vivo,
show
that
both
tests
have
similar
sensitivity
anxiety-like
states.
Finally,
evokes
higher
levels
cortisol,
likely
representing
more
stressful
procedure
than
LDT.
Collectively,
our
study
reappraises
studying
states
zebrafish,
emphasizes
their
developing
neurobehavioral
research.
These
findings
can
help
optimize
screening
procedures
by
choosing
appropriate
models
testing
anxiolytic
or
anxiogenic
drugs.
Environmental Sciences Europe,
Год журнала:
2018,
Номер
30(1)
Опубликована: Дек. 1, 2018
The
numbers
of
potential
neurotoxicants
in
the
environment
are
raising
and
pose
a
great
risk
for
humans
environment.
Currently
neurotoxicity
assessment
is
mostly
performed
to
predict
prevent
harm
human
populations.
Despite
all
efforts
invested
last
years
developing
novel
vitro
or
silico
test
systems,
vivo
tests
with
rodents
still
only
accepted
Europe.
an
increasing
number
reports
species
showing
altered
behaviour,
not
required
therefore
performed.
Considering
environmental
contaminants
neurotoxic
potential,
eco-neurotoxicity
should
be
also
considered
assessment.
In
order
do
so
systems
needed
that
can
cope
differences
within
ecosystems.
field,
online-biomonitoring
using
behavioural
information
could
used
detect
effects
effect-directed
analyses
applied
identify
causing
effect.
Additionally,
toxic
pressure
calculations
combination
mixture
modelling
use
chemical
monitoring
data
adverse
prioritize
pollutants
laboratory
testing.
Cheminformatics
based
on
computational
toxicological
from
studies
help
neurotoxicants.
An
array
assays
covering
different
modes
action
screen
compounds
neurotoxicity.
selection
guided
by
AOPs
relevant
eco-neurotoxicity.
able
perform
eco-neurotoxicity,
methods
need
focus
most
sensitive
ecosystem.
A
battery
trophic
levels
might
best
approach.
To
implement
into
European
assessment,
cheminformatics
screening
as
first
approach
eco-neurotoxic
pollutants.
second
step,
small
assess
risks
Heliyon,
Год журнала:
2023,
Номер
9(3), С. e14557 - e14557
Опубликована: Март 1, 2023
Understanding
the
detailed
mechanism
behind
every
human
disease,
disorder,
defect,
and
deficiency
is
a
daunting
task
concerning
clinical
diagnostic
tools
for
patients.
Hence,
closely
resembling
living
or
simulated
model
of
paramount
interest
development
testing
probable
novel
drug
rectifying
conditions
pertaining
to
various
ailments.
The
animal
that
can
be
easily
genetically
manipulated
suit
study
therapeutic
motive
an
indispensable
asset
within
last
few
decades,
zebrafish
models
have
proven
their
effectiveness
by
becoming
such
potent
disease
with
use
being
extended
avenues
research
understand
underlying
mechanisms
diseases.
As
are
explored
as
animals
in
understanding
molecular
basis
genetics
many
diseases
owing
70%
genetic
homology
between
genes;
new
fascinating
facts
about
surfaced,
establishing
it
very
powerful
tool
upcoming
research.
These
prospective
areas
near
future
using
model.
In
this
review,
against
several
osteoporosis,
atrial
fibrillation,
Noonan
syndrome,
leukemia,
autism
spectrum
disorders,
etc.
has
been
discussed.
British Journal of Pharmacology,
Год журнала:
2017,
Номер
174(13), С. 1925 - 1944
Опубликована: Фев. 20, 2017
Despite
the
high
prevalence
of
neuropsychiatric
disorders,
their
aetiology
and
molecular
mechanisms
remain
poorly
understood.
The
zebrafish
(
Danio
rerio
)
is
increasingly
utilized
as
a
powerful
animal
model
in
neuropharmacology
research
vivo
drug
screening.
Collectively,
this
makes
useful
tool
for
discovery
identification
disordered
pathways.
Here,
we
discuss
models
selected
human
disorders
drug‐induced
phenotypes.
As
well
covering
broad
range
brain
(from
anxiety
psychoses
to
neurodegeneration),
also
summarize
recent
developments
genetics
small
molecule
screening,
which
markedly
enhance
disease
modelling
novel
targets.
