Fluorescence lifetime enables high-resolution analysis of neuromodulator dynamics across time and animals

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Сен. 30, 2022

ABSTRACT The dynamics of neuromodulators are essential for their functions. Optical sensors have transformed the study because they capture neuromodulator with high spatial and temporal resolution. However, fluorescence intensity-based restricted to measure acute changes within one animal over a short period intensity varies sensor expression level excitation light fluctuation. In contrast, lifetime is impervious or power, allowing comparison between individuals across long periods. Here, we discover response in multiple sensors. Using acetylcholine GRAB ACh3.0 investigate power measurement, find that correlates behavior states accurately despite varying weeks animals. Thus, enables at resolution animals chronic time scales.

Язык: Английский

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65 years of research on dopamine's role in classical fear conditioning and extinction: A systematic review

European Journal of Neuroscience, Год журнала: 2023, Номер 59(6), С. 1099 - 1140

Опубликована: Окт. 17, 2023

Abstract Dopamine, a catecholamine neurotransmitter, has historically been associated with the encoding of reward, whereas its role in aversion received less attention. Here, we systematically gathered vast evidence dopamine simplest forms aversive learning: classical fear conditioning and extinction. In past, crude methods were used to augment or inhibit study relationship More advanced techniques such as conditional genetic, chemogenic optogenetic approaches now provide causal for dopamine's these learning processes. Dopamine neurons encode conditioned stimuli during extinction convey signal via activation D 1–4 receptor sites particularly amygdala, prefrontal cortex striatum. The coordinated receptors allows continuous formation, consolidation, retrieval updating memory dynamic reciprocal manner. Based on reviewed literature, conclude that is crucial contributes way comparable reward.

Язык: Английский

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Dopamine neurotransmitter determination using graphite sheet–graphene nano-sensor

Deleted Journal, Год журнала: 2024, Номер 9(1-2), С. 125 - 135

Опубликована: Март 25, 2024

In this study, graphene oxide was electrochemically deposited and reduced on a graphite sheet. The electrode surface morphology studied by scanning electron microscopy. performance of the modified in detecting dopamine investigated. results indicate that electrodeposition onto increases oxidation current, decreases overpotential, reduces charge transfer resistance. efficiency detection evaluated using cyclic voltammetry, differential pulse electrochemical impedance spectroscopy. effects electrolyte pH, potential scan rate, reproducibility, selectivity were Furthermore, for stability test, chronoamperometry technique performed, demonstrated has excellent stability. This provides low limit 68 nM wide linear range from 0.1 to 1000 µM. For real sample analysis, measurements successfully conducted ampule as cardiovascular drug. nanosensor takes advantage simple rapid fabrication process at cost.

Язык: Английский

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Striatal Dopamine Contributions to Skilled Motor Learning

Journal of Neuroscience, Год журнала: 2024, Номер 44(26), С. e0240242024 - e0240242024

Опубликована: Май 28, 2024

Coordinated multijoint limb and digit movements-"manual dexterity"-underlie both specialized skills (e.g., playing the piano) more mundane tasks tying shoelaces). Impairments in dexterous skill cause significant disability, as occurs with motor cortical injury, Parkinson's disease, a range of other pathologies. Clinical observations, well basic investigations, suggest that corticostriatal circuits play critical role learning performing skills. Furthermore, dopaminergic signaling these regions is implicated synaptic plasticity learning. Nonetheless, striatal dopamine skilled remains poorly understood. Here, we use fiber photometry paired genetically encoded sensor to investigate release male female mice they learn perform reaching task. Dopamine rapidly increases during reach peaks near pellet consumption. In dorsolateral striatum, dynamics are faster than dorsomedial ventral striatum. Across training, performance improves, shifts from consumption cues predict availability, particularly medial areas prediction errors present across reduced after an unsuccessful reach. These findings show behaviors change differentially regulated subregions.

Язык: Английский

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Dopamine transmission at D1 and D2 receptors in the nucleus accumbens contributes to the expression of incubation of cocaine craving

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 30, 2024

Relapse represents a consistent clinical problem for individuals with substance use disorder. In the incubation of craving model persistent and relapse, cue-induced drug seeking progressively intensifies or 'incubates' during first weeks abstinence from self-administration then remains high months. Previously, we others have demonstrated that expression incubated cocaine requires strengthening excitatory synaptic transmission in nucleus accumbens core (NAcc). However, despite importance dopaminergic signaling NAcc motivated behavior, little is known about role dopamine (DA) plays craving. Here used fiber photometry to measure DA transients male female rats tests conducted early self-administration, prior incubation, late abstinence, after has plateaued. We observed time-locked responding but their magnitude did not differ significantly when measured versus tests. Next, tested functional these by injecting receptor antagonists into just before test. Blockade either D1 D2 receptors reduced incubation. found no main effect sex our experiments. These results suggest contributes emergence this reflects changes postsynaptic responsiveness rather than presynaptic alterations.

Язык: Английский

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