Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 29, 2024
Abstract
CD11c-positive
(CD11c
+
)
microglia
have
attracted
considerable
attention
because
of
their
potential
implications
in
central
nervous
system
(CNS)
development,
homeostasis,
and
disease.
However,
the
spatiotemporal
dynamics
proportion
CD11c
individual
CNS
regions
are
poorly
understood.
Here,
we
investigated
six
(forebrain,
olfactory
bulb,
diencephalon/midbrain,
cerebellum,
pons/medulla,
spinal
cord)
from
developmental
to
adult
stages
by
flow
cytometry
immunohistochemical
analyses
using
a
reporter
transgenic
mouse
line,
Itgax-Venus
.
We
found
that
total
varied
between
during
postnatal
development.
Specifically,
was
high
bulb
cerebellum
at
day
P(4)
P7,
respectively,
approximately
half
were
The
declined
sharply
all
P14,
low
percentage
persisted
over
P56.
In
cord,
also
P4
but
increased
again
P21
thereafter.
Interestingly,
distribution
pattern
cord
markedly
changed
gray
matter
white
P21.
Collectively,
our
findings
reveal
differences
among
early
development
normal
mice.
These
improve
understanding
nature
microglial
heterogeneity
its
CNS.
Journal of Pharmacological Sciences,
Год журнала:
2024,
Номер
154(4), С. 312 - 315
Опубликована: Фев. 27, 2024
We
previously
identified
a
spinal
astrocyte
population
that
expresses
hairy
and
enhancer
of
split
5
(Hes5)
is
selectively
present
in
superficial
laminae
mice.
However,
it
was
unclear
whether
such
heterogeneity
commonly
observed
across
species.
Using
adeno-associated
viral
(AAV)
vectors
incorporating
rat
Hes5
promotor
(AAV-Hes5P),
we
found
AAV-Hes5P-captured
astrocytes
were
located
the
rats.
Furthermore,
activation
AAV-Hes5P+
elicited
allodynia-like
behavior
increased
c-FOS+
cells
laminae.
Thus,
laminar-selective
Hes5+
are
conserved
beyond
species
have
capability
to
convert
tactile
information
nociceptive.
Journal of Pharmacology and Experimental Therapeutics,
Год журнала:
2024,
Номер
390(2), С. 213 - 221
Опубликована: Май 22, 2024
Following
colonic
inflammation,
the
uninjured
bladder
afferent
neurons
are
also
activated.
The
mechanisms
and
pathways
underlying
this
sensory
neuron
cross-activation
(from
injured
to
neurons)
not
fully
understood.
Colonic
reside
in
same
spinal
segments
separated
by
satellite
glial
cells
(SGCs)
extracellular
matrix
dorsal
root
ganglia
(DRG).
SGCs
communicate
with
a
bidirectional
fashion.
This
review
summarizes
differentially
regulated
genes/proteins
DRG
explores
role
of
regulation
crosstalk
visceral
cross-organ
sensitization.
highlights
paracrine
mediating
neuron-SGC
SGC-neuron
coupling
an
emphasis
on
neurotrophins
purinergic
systems.
Finally,
I
discuss
results
from
recent
RNAseq
profiling
reveal
useful
molecular
markers
for
characterization,
functional
study,
therapeutic
targets
SGCs.
SIGNIFICANCE
STATEMENT:
Satellite
largest
subtypes
play
critical
crosstalk,
mechanism
colon-bladder
cross-sensitization.
Identification
novel
unique
can
advance
discovery
treatment
chronic
pain
including
comorbidity.
Inflammation Research,
Год журнала:
2024,
Номер
73(10), С. 1711 - 1726
Опубликована: Авг. 2, 2024
Neuropathic
pain
is
a
chronic
condition
characterized
by
aberrant
signaling
within
the
somatosensory
system,
affecting
millions
of
people
worldwide
with
limited
treatment
options.
Herein,
we
aim
at
investigating
potential
sigma-1
receptor
(σ1R)
antagonist
in
managing
neuropathic
pain.
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 29, 2024
Abstract
CD11c-positive
(CD11c
+
)
microglia
have
attracted
considerable
attention
because
of
their
potential
implications
in
central
nervous
system
(CNS)
development,
homeostasis,
and
disease.
However,
the
spatiotemporal
dynamics
proportion
CD11c
individual
CNS
regions
are
poorly
understood.
Here,
we
investigated
six
(forebrain,
olfactory
bulb,
diencephalon/midbrain,
cerebellum,
pons/medulla,
spinal
cord)
from
developmental
to
adult
stages
by
flow
cytometry
immunohistochemical
analyses
using
a
reporter
transgenic
mouse
line,
Itgax-Venus
.
We
found
that
total
varied
between
during
postnatal
development.
Specifically,
was
high
bulb
cerebellum
at
day
P(4)
P7,
respectively,
approximately
half
were
The
declined
sharply
all
P14,
low
percentage
persisted
over
P56.
In
cord,
also
P4
but
increased
again
P21
thereafter.
Interestingly,
distribution
pattern
cord
markedly
changed
gray
matter
white
P21.
Collectively,
our
findings
reveal
differences
among
early
development
normal
mice.
These
improve
understanding
nature
microglial
heterogeneity
its
CNS.
