Transcriptome analysis of non-coding RNAs and mRNAs in the dorsal root ganglion of peripheral nerve injury-induced neuropathic pain
Abstract
Background:
Maladaptive
changes
in
gene
expression
at
transcriptional
level
dorsal
root
ganglia
(DRGs)
after
nerve
injury
are
critical
for
neuropathic
pain
genesis.
Emerging
evidence
reveals
the
important
role
of
non-coding
RNAs
(ncRNAs)
regulating
transcription.
Recent
studies
also
have
showed
contribution
ncRNAs
to
pain.
However,
profile
DRGs
and
potential
regulatory
mechanism
peripheral
injury-induced
not
fully
clear.
Methods:
We
used
bCCI
model
induced
by
chronic
constriction
bilateral
sciatic
nerves
study
functional
micro
(miRNAs),
long
(lncRNAs),
circular
(circRNAs)
messenger
RNA
(mRNA)
sequencing
bioinformatics
analysis.
Results:
A
total
47
miRNAs,
337
lncRNAs,
32
circRNAs,
2269
mRNAs,
815
genes
were
differentially
expressed
(DE)
CCI
mice
14
days
surgery.
KEGG
analysis
demonstrated
nociception
related
signaling
pathways
significantly
enriched
DEncRNAs,
including
Rap1,
Ras,
Hippo
pathway.
GO
neuron
biological
process,
membrane
cell
components,
binding
molecular
functions
enriched.
The
competing
endogenous
(ceRNA)
network
DEmiRNA-DEmRNA,
DElncRNA-DEmRNA,
DEcircRNA-DEmiRNA
existed
with
injury.
In
addition,
81
pain-related
DE
had
protein-protein
interactions
(PPI)
each
other.
Conclusion:
Our
findings
indicated
that
involved
development
DEncRNAs
may
provide
us
a
new
perspective
research
become
target
treatment.
Research Square (Research Square), Год журнала: 2024, Номер unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
