Bone marrow microenvironment-targeted yolk-shell nanoparticle enhances acute myeloid leukemia chemotherapy by preventing endothelial cell/ECM-mediated adhesion

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 164084 - 164084

Опубликована: Май 1, 2025

Язык: Английский

---

Network-based analysis and experimental validation of identified natural compounds from Yinchen Wuling San for acute myeloid leukemia

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Май 30, 2025

Objective Traditional Chinese medicine (TCM) has garnered attention for its potential in cancer therapy. Yinchen Wuling San (YWLS), a classical herbal formula, been traditionally used liver-related conditions, but bioactive components and molecular mechanisms relevant to hematologic malignancies such as acute myeloid leukemia (AML) remain unclear. This study aims identify the active compounds targets of context AML through network pharmacology analysis, experimentally validate effects selected candidate models. Methods Active ingredients from six YWLS herbs were screened via TCMSP database using oral bioavailability ≥30% DL ≥0.18 thresholds. Targets predicted SwissTargetPrediction, AML-related genes obtained DisGeNET GeneCards. Key overlapping analyzed STRING PPI networks GO/KEGG enrichment. Molecular docking was performed between three core (genkwanin, isorhamnetin, quercetin) hub proteins (e.g., SRC) Sybyl-X. ADME profiles SwissADME, dynamics simulations (GROMACS) assessed complex stability. These further evaluated vitro (viability, apoptosis, cell cycle, RT-qPCR, flow cytometry) vivo an xenograft mouse model. Results Of 621 targets, 113 overlapped with 1,247 genes. analysis identified genes, including AKT1, SRC, EGFR. Enrichment highlighted PI3K-AKT, MAPK, JAK-STAT pathways. Genkwanin, quercetin target strong affinities. suggested favorable pharmacokinetics, confirmed structural In , these exhibited dose-dependent cytotoxicity, induced modulated downregulated SRC expression. Notably, Genkwanin promoted CD8 + T proliferation inhibited growth, improving survival Conclusion compounds, particularly exhibit significant anti-leukemic activity apoptosis induction, cycle modulation, promote cells proliferation. emerges promising therapeutic AML, warranting clinical investigation.

Язык: Английский

---

Synthetic and biological nanoparticles for cancer immunotherapy

Biomaterials Science, Год журнала: 2024, Номер 12(23), С. 5933 - 5960

Опубликована: Янв. 1, 2024

Cancer is becoming the main public health problem globally. Conventional chemotherapy approaches are slowly being replaced or complemented by new therapies that avoid loss of healthy tissue, limit off-targets, and eradicate cancer cells. Immunotherapy nowadays an important strategy for treatment, uses host's anti-tumor response activating immune system increasing effector cell number, while, minimizing cancer's immune-suppressor mechanisms. Its efficacy still limited poor therapeutic targeting, low immunogenicity, antigen presentation deficiency, impaired T-cell trafficking infiltration, heterogeneous microenvironment, multiple checkpoints unwanted side effects, which could benefit from improved delivery systems, able to release immunotherapeutic agents tumor microenvironment Nanoparticles (NPs) immunotherapy (Nano-IT), have a huge potential solve these limitations. Natural and/or synthetic, targeted stimuli-responsive nanoparticles can be used deliver in their native conformations site interest enhance antitumor activity. They also as co-adjuvants activity IT These engineered natural context cell-derived extracellular vesicles (EVs) exosomes fully synthetic. In this review, detailed SWOT analysis done through comparison engineered-synthetic naturaly-derived terms current future use immunotherapy.

Язык: Английский

---

Prediction of acute myeloid leukemia prognosis based on autophagy features and characterization of its immune microenvironment

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 22, 2024

Background Autophagy promotes the survival of acute myeloid leukemia (AML) cells by removing damaged organelles and proteins protecting them from stress-induced apoptosis. Although many studies have identified candidate autophagy genes associated with AML prognosis, there are still great challenges in predicting prognosis patients. Therefore, it is necessary to identify more novel gene markers improve utilizing information at molecular level. Methods In this study, Random Forest, SVM XGBoost algorithms were utilized linked respectively. Subsequently, six (TSC2, CALCOCO2, BAG3, UBQLN4, ULK1 DAPK1) that significantly patients’ overall (OS) using Lasso-Cox regression analysis. A prediction model incorporating these was then developed. addition, immunological microenvironment analysis performed study. Results The experimental results showed predictive had good ability. After adjusting for clinicopathologic parameters, feature proved an independent prognostic predictor validated external sample set. Analysis differentially expressed patients high-risk low-risk groups enriched immune-related pathways such as humoral immune response, T cell differentiation thymus lymphocyte differentiation. Then infiltration cellular abundance CD4+ memory activated, NK activated group lower than group. Conclusion This study systematically analyzed autophagy-related (ARGs) developed predictors related OS AML, thus accurately assessing not only helps assessment therapeutic outcome patients, but may also provide new help future research clinical applications.

Язык: Английский

---

Epigenetic alterations in AML: Deregulated functions leading to new therapeutic options

International review of cell and molecular biology, Год журнала: 2024, Номер unknown, С. 27 - 75

Опубликована: Янв. 1, 2024

Язык: Английский

---

From oxidative stress to metabolic dysfunction: The role of TRPM2

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 284, С. 138081 - 138081

Опубликована: Ноя. 26, 2024

Язык: Английский

---