Alteration of the Antifungal Action Mechanism Due to Structural Changes in the Antimicrobial Peptide, HnMc DOI Creative Commons

K Cho,

Jae Ho Lee, Min Young Lee

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(3), С. 1307 - 1307

Опубликована: Янв. 27, 2025

The rapid induction of drug resistance is considered a fatal drawback conventional antibiotics and requires the continuous development new antibiotics. Accordingly, antibacterial peptides (AMPs) have attracted interest as next-generation many studies been conducted. However, much remains unknown regarding mechanism AMPs effects amino acid sequence changes. We compared structures antifungal HnMc-W (F1W substitution, straight alpha-helical structure), HnMc-WP1 (S9P bending HnMc-WP2 (addition PXXP motif, helix-to-helix structure) to those parent hybrid AMP (HnMc) their action. most active was HnMc-WP2, which exhibited an effect via membranolytic action on fungal cell membrane. others inhibited growth by inducing apoptosis through reactive oxygen species production caused mitochondrial damage. This study proposes addition ‘PXXP’ motif in design acting membranes.

Язык: Английский

Alteration of the Antifungal Action Mechanism Due to Structural Changes in the Antimicrobial Peptide, HnMc DOI Creative Commons

K Cho,

Jae Ho Lee, Min Young Lee

и другие.

Applied Sciences, Год журнала: 2025, Номер 15(3), С. 1307 - 1307

Опубликована: Янв. 27, 2025

The rapid induction of drug resistance is considered a fatal drawback conventional antibiotics and requires the continuous development new antibiotics. Accordingly, antibacterial peptides (AMPs) have attracted interest as next-generation many studies been conducted. However, much remains unknown regarding mechanism AMPs effects amino acid sequence changes. We compared structures antifungal HnMc-W (F1W substitution, straight alpha-helical structure), HnMc-WP1 (S9P bending HnMc-WP2 (addition PXXP motif, helix-to-helix structure) to those parent hybrid AMP (HnMc) their action. most active was HnMc-WP2, which exhibited an effect via membranolytic action on fungal cell membrane. others inhibited growth by inducing apoptosis through reactive oxygen species production caused mitochondrial damage. This study proposes addition ‘PXXP’ motif in design acting membranes.

Язык: Английский

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