"Microfluidic vessels-on-chips for vascular disease modelling" DOI
Heleen Middelkamp

Опубликована: Июнь 22, 2023

Chapter 1General introduction endothelial cell damage 27,29,47,57 .When modelling or inflammatory responses, standard read-outs are fluorescent staining of markers.However, this does not show the functional differences in blood vessel such as monocyte adhesion and thrombus formation which will make them more physiologically relevant can assist assessing patient specific responses.Furthermore, demand for standardized quantifiable is rising 58 .Some BVoC models with perfusion assays already existence [59][60][61][62][63][64] , however standardization, quantification read-outs, systematically setting up co-cultures these needs to be optimized, making measurements translatable comparable 58,[65][66][67] .Current often use fluorescently labelled markers an end-point microscopic measurement 62,68,69 .Thrombus development BVoCs difficult observe real time using assays, furthermore, images give a 2D view thrombus.Therefore non-invasive method measuring clot over time, multiple dimensions, addition understanding clotting mechanisms 70,71 .Many challenges remain creating models, vascular disease modelling, include types possibility read-outs.This thesis aims contribute field by presenting new chip designs, methods co-culturing cells, quantifyable read-outs. Thesis outlineThis presents research towards modelling.Using different designs types, we ability create several vessel-on-chip (VoC) and/or organs read-outs.Chapter 2 consists two parts.The first part describes importance early involvement stakeholders OoCs.It shows stakeholder opinions, overcome process.The next summarizes VoC currently used.Next that discuss possibilities vivo vitro where VoCs still lacking.

Язык: Английский

The Product Science of Electrically Heated Tobacco Products: An Updated Narrative Review of the Scientific Literature DOI Open Access

Sarah F Cordery,

Keith Thompson, Matthew Stevenson

и другие.

Cureus, Год журнала: 2024, Номер unknown

Опубликована: Май 28, 2024

Heated tobacco products represent a novel category of in which consumable is heated to temperature that releases nicotine from the leaf but not sufficient cause combustion. may therefore have potential be less harmful alternative for adult smokers who would otherwise continue smoke cigarettes, as their use should result exposure substantially fewer and lower levels toxicants. This update represents two-year extension our previous narrative review, covered peer-reviewed journal articles published up August 31, 2021. The scientific evidence between 2021 2023 continues indicate aerosols produced contain potentially constituents these observed reductions consistently translate reduced biological effects both vitro vivo toxicological studies. Biomarker clinical data studies product controlled within setting suggest changes biomarkers exposure, harm, endpoints indicating harm with switching exclusive smokers. Overall, available offer promise such, conclusions original review remain valid.

Язык: Английский

Процитировано

9
Innovative Atherosclerosis Models: Advancing Pathophysiology and Translational Research DOI Creative Commons

Huiting Jiang,

Yukun Liao,

Mengliang Zhu

и другие.

Research, Год журнала: 2025, Номер 8

Опубликована: Янв. 1, 2025

Atherosclerosis (AS) is a chronic inflammatory condition influenced by glucose and lipid disorders, oxidative stress, thrombosis, reflecting the complexity of its pathological process. The development accurate experimental models that simulate human AS essential for understanding initiation progression. This review summarizes current research analyzes their specific application scenarios. We discuss tissue-engineered blood vessels (TEBVs) vessels-on-a-chip (VoCs), which leverage tissue engineering precise microenvironmental control to construct in vitro closely resemble structure function AS. Isolated vessel segments from live animals provide valuable tool investigating due physiological similarity, controllability, reproducibility. further outlines construction animal through high-fat diets gene-editing techniques, highlighting how immune-inflammatory responses, mechanical arterial injury, hemodynamic changes accelerate model development. comprehensive analysis highlights potential revolutionize theranostic applications clinical translational research, paving way more personalized effective treatments near future.

Язык: Английский

Процитировано

1
In vitro toxicological assessment of aerosols from indirectly heated tobacco products using a solvent-free extraction method DOI Creative Commons
Tsuneo Hashizume, Haruna Yamamoto, Hiroshi Itô

и другие.

Food and Chemical Toxicology, Год журнала: 2025, Номер unknown, С. 115333 - 115333

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Optimisation of an in vitro human cardiovascular model on-a-chip for toxicological assessment of nicotine delivery products DOI Creative Commons
Fiona Chapman, Luuk de Haan,

Linda Gijzen

и другие.

Frontiers in Toxicology, Год журнала: 2024, Номер 6

Опубликована: Июнь 13, 2024

Background Smoking cigarettes is a cause of serious diseases in smokers, including cardiovascular disease. Through pathway endothelial dysfunction, lipid infiltration, macrophage recruitment and vascular remodeling, atherosclerosis fundamental the development most diseases. There an increasing number next-generation products (NGP) which provide potentially reduced harm forms nicotine delivery to adult smokers. This study aimed optimise vitro model assess such products. Human Coronary Artery Endothelial Cells (HCAECs) were cultured on OrganoPlate ® 2-lane chip (Mimetas BV) combined with THP-1 monocytes under flow conditions. Methods An aqueous aerosol extract from 1R6F reference cigarette was compared two categories NGP, (a heated tobacco product (HTP) electronic system (ENDS)), relative effects select atherogenic endpoints (oxidative stress, monocyte adhesion, ICAM-1 expression, inflammatory markers). Following exposure extracts, resulting conditioned medium then added HCAEC vessels. Results consistently potent test article, eliciting observed responses at 4x lower concentrations than applied for both HTP ENDS. The more ENDS across all endpoints, however, articles increased adhesion. did not appear be main driver this could due replicate variability. Upon comparison extract-only control exposure, THP-1-medium pre-conditioning important mediator observed. Conclusion In conclusion, data suggests that NGP containing primary chemical constituents exhibit marked reduction biological activity early key events associated atherogenesis when cigarette, adding weight evidence potential

Язык: Английский

Процитировано

1
RNA sequencing analysis of early-stage atherosclerosis in vascular-on-a-chip and its application for comparing combustible cigarettes with heated tobacco products DOI Creative Commons
Kazuhiro �Ohashi,

Ayaka Hayashida,

Atsuko Nozawa

и другие.

Current Research in Toxicology, Год журнала: 2024, Номер 6, С. 100163 - 100163

Опубликована: Янв. 1, 2024

Our previous study showed promising results in replicating early-stage atherosclerosis when vascular endothelial cells (VECs) were exposed to cigarette smoke (CS) extract via M0 macrophages. We used an organ-on-a-chip system as alternative animal testing model atherosclerosis, which is a complex disease involving and immune cell communications. By incorporating macrophages into the vascular-on-a-chip system, we aimed mimic indirect effects of inhalable substances, such CS, on VECs. In current study, further examined suitability our

Язык: Английский

Процитировано

0
Applying new approach methodologies to assess next-generation tobacco and nicotine products DOI Creative Commons
David Thorne, Damian McHugh, Liam Simms

и другие.

Frontiers in Toxicology, Год журнала: 2024, Номер 6

Опубликована: Июнь 13, 2024

In vitro toxicology research has accelerated with the use of in silico , computational approaches and human tissue systems, facilitating major improvements evaluating safety health risks novel consumer products. Innovation molecular cellular biology shifted testing paradigms, less reliance on low-throughput animal data greater medium- high-throughput screening approaches. These new approach methodologies (NAMs) are being implemented other industry sectors for chemical testing, candidate drugs prototype products, driven by need reliable, human-relevant Routine toxicological methods largely unchanged since development over 50 years ago, using high-doses often employing vivo testing. Several disadvantages encountered conducting or extrapolating from studies due to differences metabolism exposure. The last decade saw considerable advancement tools capabilities, challenges next will be integrating these platforms into applied product acceptance regulatory bodies. Governmental validation agencies have launched frameworks “roadmaps” support agile NAMs. Next-generation tobacco nicotine products (NGPs) potential offer reduced smokers compared cigarettes. include heated (HTPs) that heat but do not burn tobacco; vapor also termed electronic delivery systems (ENDS), an e-liquid produce inhalable aerosol; oral smokeless (e.g., Swedish-style snus) tobacco-free pouches. With increased availability NGPs requirement scientific approval, NAMs can supplement assessment NGPs. This review explores how assess NGPs, highlighting key considerations, including appropriate model deploying hazard identification, importance test article characterization. opportunity fit-for-purpose method standardization discussed, value cross-industry collaborations. Supporting accepted bodies could lead implementation NGP

Язык: Английский

Процитировано

0
Multidisciplinary approaches in electronic nicotine delivery systems pulmonary toxicology: emergence of living and non-living bioinspired engineered systems DOI Creative Commons
Kambez H. Benam

Communications Engineering, Год журнала: 2024, Номер 3(1)

Опубликована: Сен. 3, 2024

Abstract Technology-based platforms offer crucial support for regulatory agencies in overseeing tobacco products to enhance public health protection. The use of electronic nicotine delivery systems (ENDS), such as cigarettes, has surged exponentially over the past decade. However, understanding impact ENDS on lung remains incomplete due scarcity physiologically relevant technologies evaluating their toxicity. This review examines societal and impacts ENDS, prevalent preclinical approaches pulmonary space, application emerging Organ-on-Chip bioinspired robotics assessing respiratory It highlights challenges inhalation toxicology value multidisciplinary bioengineering generating reliable, human-relevant data at an accelerated pace.

Язык: Английский

Процитировано

0
Opportunities for Microphysiological Systems from the View of Japanese Industries DOI Creative Commons

Hitoshi Naraoka,

Tomoko Iguchi,

Kosuke Harada

и другие.

Drug Metabolism and Pharmacokinetics, Год журнала: 2024, Номер 60, С. 101034 - 101034

Опубликована: Ноя. 9, 2024

Язык: Английский

Процитировано

0
"Microfluidic vessels-on-chips for vascular disease modelling" DOI
Heleen Middelkamp

Опубликована: Июнь 22, 2023

Chapter 1General introduction endothelial cell damage 27,29,47,57 .When modelling or inflammatory responses, standard read-outs are fluorescent staining of markers.However, this does not show the functional differences in blood vessel such as monocyte adhesion and thrombus formation which will make them more physiologically relevant can assist assessing patient specific responses.Furthermore, demand for standardized quantifiable is rising 58 .Some BVoC models with perfusion assays already existence [59][60][61][62][63][64] , however standardization, quantification read-outs, systematically setting up co-cultures these needs to be optimized, making measurements translatable comparable 58,[65][66][67] .Current often use fluorescently labelled markers an end-point microscopic measurement 62,68,69 .Thrombus development BVoCs difficult observe real time using assays, furthermore, images give a 2D view thrombus.Therefore non-invasive method measuring clot over time, multiple dimensions, addition understanding clotting mechanisms 70,71 .Many challenges remain creating models, vascular disease modelling, include types possibility read-outs.This thesis aims contribute field by presenting new chip designs, methods co-culturing cells, quantifyable read-outs. Thesis outlineThis presents research towards modelling.Using different designs types, we ability create several vessel-on-chip (VoC) and/or organs read-outs.Chapter 2 consists two parts.The first part describes importance early involvement stakeholders OoCs.It shows stakeholder opinions, overcome process.The next summarizes VoC currently used.Next that discuss possibilities vivo vitro where VoCs still lacking.

Язык: Английский

Процитировано

0