Early Biomarkers for Detecting Subclinical Exposure to Fumonisin B1, Deoxynivalenol, and Zearalenone in Broiler Chickens
Toxins,
Год журнала:
2024,
Номер
17(1), С. 1 - 1
Опубликована: Дек. 24, 2024
Identifying
biomarkers
of
mycotoxin
effects
in
chickens
will
provide
an
opportunity
for
early
intervention
to
reduce
the
impact
mycotoxicosis.
This
study
aimed
identify
whether
serum
enzyme
concentrations,
gut
integrity,
and
liver
miRNAs
can
be
potential
fumonisin
B1
(FB1),
deoxynivalenol
(DON),
zearalenone
(ZEA)
toxicity
broiler
birds
as
14
days
after
exposure.
A
total
720
male
chicks
were
distributed
six
treatment
groups:
T1:
control
group
(basal
diet),
T2
(2
FB1
+
2.5
DON
0.9
ZEA),
T3
(5
0.4
0.1
T4
(9
3.5
0.7
T5
(17
1.0
0.2
T6
(21
3.0
all
mg/kg
diet.
On
d14,
there
no
significant
differences
body
weight
gain
(BWG)
groups
when
compared
(p
>
0.05),
whereas
on
d21,
showed
significantly
reduced
BWG
<
0.05).
upregulation
miRNAs,
gga-let-7a-5p
(14.17-fold),
gga-miR-9-5p
(7.05-fold),
gga-miR-217-5p
(16.87-fold),
gga-miR-133a-3p
(7.41-fold),
gga-miR-215-5p
(6.93-fold)
0.05)
elevated
fluorescein
isothiocyanate-dextran
(FITC-d)
aspartate
aminotransferase
(AST),
creatine
kinase
(CK)
levels
exhibited
phosphorus,
glucose,
potassium,
while
protein,
FITC-d,
AST,
CK
increased
These
findings
suggest
that
CK,
could
serve
detecting
exposure
chickens.
Язык: Английский
Effects of the Maximum Recommended Levels of Fumonisins in the EU on Oxylipin Profiles in the Liver and Brain of Chickens
Antioxidants,
Год журнала:
2024,
Номер
14(1), С. 19 - 19
Опубликована: Дек. 27, 2024
This
study
aimed
to
assess
the
effects
of
a
diet
containing
20.8
mg
FB1
+
FB2/kg
over
four
and
nine
days
on
oxylipin
(OL)
profiles
in
liver
brain
chickens.
A
total
96
OLs,
derived
from
seven
polyunsaturated
fatty
acids
(PUFAs)
via
cyclooxygenase
(COX),
lipoxygenase
(LOX),
cytochrome
P450
(P450),
non-enzymatic
pathways,
were
measured
using
HPLC-MS/MS.
In
liver,
significant
increase
epoxide
P450-derived
OLs
was
detected
by
day
4,
with
smaller
but
notable
increases
COX-
LOX-derived
9.
These
alterations
independent
whether
parent
PUFA
ω6
or
ω3.
However,
18-carbon
(C18)
PUFAs,
such
as
linoleic
acid
alpha-linolenic
acid,
showed
greater
compared
those
C20
C22
PUFAs.
The
diol/epoxide
ratios
decreased
at
days,
suggesting
that
fumonisins
did
not
induce
an
inflammatory
response.
brain,
most
discriminative
ω3-PUFAs,
including
docosahexaenoic
docosapentaenoic
LOX
pathway.
By
several
arachidonic
spanning
all
enzymatic
became
discriminative.
general,
4
then
returned
initial
levels.
Taken
together,
these
results
show
strong
are
both
specific
distinct.
Язык: Английский