Transcriptomic Characterization of Maturing Neurons from Human Neural Stem Cells Across Developmental Time Points DOI Creative Commons
Kimia Hosseini, Gaëtan Philippot,

Sara B. Salomonsson

и другие.

IBRO Neuroscience Reports, Год журнала: 2025, Номер 18, С. 679 - 689

Опубликована: Апрель 17, 2025

Neurodevelopmental studies employing animal models encounter challenges due to interspecies differences and ethical concerns. Maturing neurons of human origin, undergoing several developmental stages, present a powerful alternative. In this study, embryonic stem cell (H9 line) was differentiated into neural cells subsequently matured over 30 days. Ion AmpliSeq™ used for transcriptomic characterization cell-derived at multiple time points. Data analysis revealed progressive increase markers associated with neuronal development astrocyte markers, indicating the establishment co-culture accommodating both glial neurons. Transcriptomic pathway enrichment also more pronounced GABAergic phenotype in neurons, signifying their specialization toward type. The findings confirm robustness these across different passages demonstrate detailed progression through stages development. model is intended neurodevelopmental applications can be adapted investigate how genetic modifications or exposure chemicals, pharmaceuticals, other environmental factors influence maturation.

Язык: Английский

Developmental Neurotoxicity as a Case Example for a Six-Step Framework for the Sustainable Regulatory Implementation of NAMs DOI
Jonathan Blum, Kristina Bartmann,

Joyce de Paula Souza

и другие.

Current Opinion in Toxicology, Год журнала: 2025, Номер unknown, С. 100528 - 100528

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Transcriptomic Characterization of Maturing Neurons from Human Neural Stem Cells Across Developmental Time Points DOI Creative Commons
Kimia Hosseini, Gaëtan Philippot,

Sara B. Salomonsson

и другие.

IBRO Neuroscience Reports, Год журнала: 2025, Номер 18, С. 679 - 689

Опубликована: Апрель 17, 2025

Neurodevelopmental studies employing animal models encounter challenges due to interspecies differences and ethical concerns. Maturing neurons of human origin, undergoing several developmental stages, present a powerful alternative. In this study, embryonic stem cell (H9 line) was differentiated into neural cells subsequently matured over 30 days. Ion AmpliSeq™ used for transcriptomic characterization cell-derived at multiple time points. Data analysis revealed progressive increase markers associated with neuronal development astrocyte markers, indicating the establishment co-culture accommodating both glial neurons. Transcriptomic pathway enrichment also more pronounced GABAergic phenotype in neurons, signifying their specialization toward type. The findings confirm robustness these across different passages demonstrate detailed progression through stages development. model is intended neurodevelopmental applications can be adapted investigate how genetic modifications or exposure chemicals, pharmaceuticals, other environmental factors influence maturation.

Язык: Английский

Процитировано

0